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Science 292 (5520): 1379-1382

Copyright © 2001 by the American Association for the Advancement of Science

Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCFTIR1 in Mediating Auxin Response

Claus Schwechheimer,1* Giovanna Serino,1* Judy Callis,2 William L. Crosby,3 Svetlana Lyapina,4 Raymond J. Deshaies,4 William M. Gray,5 Mark Estelle,5 Xing-Wang Deng1dagger

The COP9 signalosome is an evolutionary conserved multiprotein complex of unknown function that acts as a negative regulator of photomorphogenic seedling development in Arabidopsis. Here, we show that plants with reduced COP9 signalosome levels had decreased auxin response similar to loss-of-function mutants of the E3 ubiquitin ligase SCFTIR1. Furthermore, we found that the COP9 signalosome and SCFTIR1 interacted in vivo and that the COP9 signalosome was required for efficient degradation of PSIAA6, a candidate substrate of SCFTIR1. Thus, the COP9 signalosome may play an important role in mediating E3 ubiquitin ligase-mediated responses.

1 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
2 Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
3 Plant Biotechnology Institute, National Research Council, Saskatoon SK S7N OW9, Canada.
4 Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
5 Institute for Molecular and Cellular Biology, Section of Molecular, Cellular, and Developmental Biology, University of Texas, Austin, TX 78712, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: xingwang.deng{at}

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PLANT CELL 14, 2137-2144
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Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants.
J. R. Peart, R. Lu, A. Sadanandom, I. Malcuit, P. Moffett, D. C. Brice, L. Schauser, D. A. W. Jaggard, S. Xiao, M. J. Coleman, et al. (2002)
PNAS 99, 10865-10869
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The SCFCOI1 Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis.
L. Xu, F. Liu, E. Lechner, P. Genschik, W. L. Crosby, H. Ma, W. Peng, D. Huang, and D. Xie (2002)
PLANT CELL 14, 1919-1935
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Role of SCF Ubiquitin-Ligase and the COP9 Signalosome in the N Gene-Mediated Resistance Response to Tobacco mosaic virus.
Y. Liu, M. Schiff, G. Serino, X.-W. Deng, and S. P. Dinesh-Kumar (2002)
PLANT CELL 14, 1483-1496
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The NEDD8 Pathway Is Essential for SCFbeta -TrCP-mediated Ubiquitination and Processing of the NF-kappa B Precursor p105.
R. E. Amir, K. Iwai, and A. Ciechanover (2002)
J. Biol. Chem. 277, 23253-23259
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Null Mutation of AtCUL1 Causes Arrest in Early Embryogenesis in Arabidopsis.
W.-H. Shen, Y. Parmentier, H. Hellmann, E. Lechner, A. Dong, J. Masson, F. Granier, L. Lepiniec, M. Estelle, and P. Genschik (2002)
Mol. Biol. Cell 13, 1916-1928
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Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis.
M. Holm, L.-G. Ma, L.-J. Qu, and X.-W. Deng (2002)
Genes & Dev. 16, 1247-1259
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A Breakdown in Defense Signaling.
P. N. Dodds and C. Schwechheimer (2002)
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Ubiquitination and Auxin Signaling: A Degrading Story.
S. Kepinski and O. Leyser (2002)
PLANT CELL 14, S81-S95
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The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction.
M. H. Glickman and A. Ciechanover (2002)
Physiol Rev 82, 373-428
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PLANT BIOLOGY: Enhanced: Resisting Attack.
M. Nishimura and S. Somerville (2002)
Science 295, 2032-2033
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The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance.
C. Azevedo, A. Sadanandom, K. Kitagawa, A. Freialdenhoven, K. Shirasu, and P. Schulze-Lefert (2002)
Science 295, 2073-2076
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Arabidopsis COP10 is a ubiquitin-conjugating enzyme variant that acts together with COP1 and the COP9 signalosome in repressing photomorphogenesis.
G. Suzuki, Y. Yanagawa, S. F. Kwok, M. Matsui, and X.-W. Deng (2002)
Genes & Dev. 16, 554-559
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