Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 292 (5520): 1379-1382

Copyright © 2001 by the American Association for the Advancement of Science

Interactions of the COP9 Signalosome with the E3 Ubiquitin Ligase SCFTIR1 in Mediating Auxin Response

Claus Schwechheimer,1* Giovanna Serino,1* Judy Callis,2 William L. Crosby,3 Svetlana Lyapina,4 Raymond J. Deshaies,4 William M. Gray,5 Mark Estelle,5 Xing-Wang Deng1dagger

The COP9 signalosome is an evolutionary conserved multiprotein complex of unknown function that acts as a negative regulator of photomorphogenic seedling development in Arabidopsis. Here, we show that plants with reduced COP9 signalosome levels had decreased auxin response similar to loss-of-function mutants of the E3 ubiquitin ligase SCFTIR1. Furthermore, we found that the COP9 signalosome and SCFTIR1 interacted in vivo and that the COP9 signalosome was required for efficient degradation of PSIAA6, a candidate substrate of SCFTIR1. Thus, the COP9 signalosome may play an important role in mediating E3 ubiquitin ligase-mediated responses.

1 Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520-8104, USA.
2 Section of Molecular and Cellular Biology, University of California, Davis, CA 95616, USA.
3 Plant Biotechnology Institute, National Research Council, Saskatoon SK S7N OW9, Canada.
4 Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
5 Institute for Molecular and Cellular Biology, Section of Molecular, Cellular, and Developmental Biology, University of Texas, Austin, TX 78712, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: xingwang.deng{at}yale.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Organization of a CSN5-containing Subcomplex of the COP9 Signalosome.
G. G. Kotiguda, D. Weinberg, M. Dessau, C. Salvi, G. Serino, D. A. Chamovitz, and J. A. Hirsch (2012)
J. Biol. Chem. 287, 42031-42041
   Abstract »    Full Text »    PDF »
The COP9 Signalosome: Its Regulation of Cullin-Based E3 Ubiquitin Ligases and Role in Photomorphogenesis.
C. D. Nezames and X. W. Deng (2012)
Plant Physiology 160, 38-46
   Full Text »    PDF »
SMALL ACIDIC PROTEIN1 Acts with RUB Modification Components, the COP9 Signalosome, and AXR1 to Regulate Growth and Development of Arabidopsis.
A. Nakasone, M. Fujiwara, Y. Fukao, K. K. Biswas, A. Rahman, M. Kawai-Yamada, I. Narumi, H. Uchimiya, and Y. Oono (2012)
Plant Physiology 160, 93-105
   Abstract »    Full Text »    PDF »
Deconjugation of Nedd8 from Cul1 Is Directly Regulated by Skp1-F-box and Substrate, and the COP9 Signalosome Inhibits Deneddylated SCF by a Noncatalytic Mechanism.
E. D. Emberley, R. Mosadeghi, and R. J. Deshaies (2012)
J. Biol. Chem. 287, 29679-29689
   Abstract »    Full Text »    PDF »
MLN4924 Is an Efficient Inhibitor of NEDD8 Conjugation in Plants.
J. P. Hakenjos, R. Richter, E. M. N. Dohmann, A. Katsiarimpa, E. Isono, and C. Schwechheimer (2011)
Plant Physiology 156, 527-536
   Abstract »    Full Text »    PDF »
Geminiviruses Subvert Ubiquitination by Altering CSN-Mediated Derubylation of SCF E3 Ligase Complexes and Inhibit Jasmonate Signaling in Arabidopsis thaliana.
R. Lozano-Duran, T. Rosas-Diaz, G. Gusmaroli, A. P. Luna, L. Taconnat, X. W. Deng, and E. R. Bejarano (2011)
PLANT CELL 23, 1014-1032
   Abstract »    Full Text »    PDF »
Do Trees Grow on Money? Auxin as the Currency of the Cellular Economy.
J. L. Stewart and J. L. Nemhauser (2010)
Cold Spring Harb Perspect Biol 2, a001420
   Abstract »    Full Text »    PDF »
Interactions between Auxin and Strigolactone in Shoot Branching Control.
A. Hayward, P. Stirnberg, C. Beveridge, and O. Leyser (2009)
Plant Physiology 151, 400-412
   Abstract »    Full Text »    PDF »
Using RNA Interference to Identify Specific Modifiers of a Temperature-Sensitive, Embryonic-Lethal Mutation in the Caenorhabditis elegans Ubiquitin-Like Nedd8 Protein Modification Pathway E1-Activating Gene rfl-1.
M. Dorfman, J.-E. Gomes, S. O'Rourke, and B. Bowerman (2009)
Genetics 182, 1035-1049
   Abstract »    Full Text »    PDF »
An interaction network of the mammalian COP9 signalosome identifies Dda1 as a core subunit of multiple Cul4-based E3 ligases.
M. H. Olma, M. Roy, T. Le Bihan, I. Sumara, S. Maerki, B. Larsen, M. Quadroni, M. Peter, M. Tyers, and L. Pintard (2009)
J. Cell Sci. 122, 1035-1044
   Abstract »    Full Text »    PDF »
COP9 Signalosome- and 26S Proteasome-dependent Regulation of SCFTIR1 Accumulation in Arabidopsis.
J. Stuttmann, E. Lechner, R. Guerois, J. E. Parker, L. Nussaume, P. Genschik, and L. D. Noel (2009)
J. Biol. Chem. 284, 7920-7930
   Abstract »    Full Text »    PDF »
Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 That Disrupts SCF Function at the C Terminus of CUL1 in Arabidopsis thaliana.
J. Gilkerson, J. Hu, J. Brown, A. Jones, T.-p. Sun, and J. Callis (2009)
Genetics 181, 945-963
   Abstract »    Full Text »    PDF »
The Arabidopsis COP9 Signalosome Subunit 7 Is a Model PCI Domain Protein with Subdomains Involved in COP9 Signalosome Assembly.
M. Dessau, Y. Halimi, T. Erez, O. Chomsky-Hecht, D. A. Chamovitz, and J. A. Hirsch (2008)
PLANT CELL 20, 2815-2834
   Abstract »    Full Text »    PDF »
Auxin Responses in Mutants of the Arabidopsis CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome.
E. M. N. Dohmann, M. P. Levesque, E. Isono, M. Schmid, and C. Schwechheimer (2008)
Plant Physiology 147, 1369-1379
   Abstract »    Full Text »    PDF »
The COP9/Signalosome Increases the Efficiency of von Hippel-Lindau Protein Ubiquitin Ligase-mediated Hypoxia-inducible Factor-{alpha} Ubiquitination.
Y. Miyauchi, M. Kato, F. Tokunaga, and K. Iwai (2008)
J. Biol. Chem. 283, 16622-16631
   Abstract »    Full Text »    PDF »
The Arabidopsis COP9 signalosome is essential for G2 phase progression and genomic stability.
E. M. N. Dohmann, M. P. Levesque, L. De Veylder, I. Reichardt, G. Jurgens, M. Schmid, and C. Schwechheimer (2008)
Development 135, 2013-2022
   Abstract »    Full Text »    PDF »
GLH-1, the C. elegans P granule protein, is controlled by the JNK KGB-1 and by the COP9 subunit CSN-5.
A. M. Orsborn, W. Li, T. J. McEwen, T. Mizuno, E. Kuzmin, K. Matsumoto, and K. L. Bennett (2007)
Development 134, 3383-3392
   Abstract »    Full Text »    PDF »
CIF-1, a Shared Subunit of the COP9/Signalosome and Eukaryotic Initiation Factor 3 Complexes, Regulates MEL-26 Levels in the Caenorhabditis elegans Embryo.
S. Luke-Glaser, M. Roy, B. Larsen, T. Le Bihan, P. Metalnikov, M. Tyers, M. Peter, and L. Pintard (2007)
Mol. Cell. Biol. 27, 4526-4540
   Abstract »    Full Text »    PDF »
Mutation of E1-CONJUGATING ENZYME-RELATED1 Decreases RELATED TO UBIQUITIN Conjugation and Alters Auxin Response and Development.
A. W. Woodward, S. E. Ratzel, E. E. Woodward, Y. Shamoo, and B. Bartel (2007)
Plant Physiology 144, 976-987
   Abstract »    Full Text »    PDF »
Ubiquitin, Hormones and Biotic Stress in Plants.
K. Dreher and J. Callis (2007)
Ann. Bot. 99, 787-822
   Abstract »    Full Text »    PDF »
Characterization of the VIER F-BOX PROTEINE Genes from Arabidopsis Reveals Their Importance for Plant Growth and Development.
K. M. Schwager, L. I. A. Calderon-Villalobos, E. M.N. Dohmann, B. C. Willige, S. Knierer, C. Nill, and C. Schwechheimer (2007)
PLANT CELL 19, 1163-1178
   Abstract »    Full Text »    PDF »
Role of the MPN Subunits in COP9 Signalosome Assembly and Activity, and Their Regulatory Interaction with Arabidopsis Cullin3-Based E3 Ligases.
G. Gusmaroli, P. Figueroa, G. Serino, and X. W. Deng (2007)
PLANT CELL 19, 564-581
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Regulates Skp2 Levels and Proliferation of Human Cells.
S. Denti, M. E. Fernandez-Sanchez, L. Rogge, and E. Bianchi (2006)
J. Biol. Chem. 281, 32188-32196
   Abstract »    Full Text »    PDF »
Arabidopsis CULLIN4 Forms an E3 Ubiquitin Ligase with RBX1 and the CDD Complex in Mediating Light Control of Development.
H. Chen, Y. Shen, X. Tang, L. Yu, J. Wang, L. Guo, Y. Zhang, H. Zhang, S. Feng, E. Strickland, et al. (2006)
PLANT CELL 18, 1991-2004
   Abstract »    Full Text »    PDF »
LucTrap Vectors Are Tools to Generate Luciferase Fusions for the Quantification of Transcript and Protein Abundance in Vivo.
L. I. A. Calderon-Villalobos, C. Kuhnle, H. Li, M. Rosso, B. Weisshaar, and C. Schwechheimer (2006)
Plant Physiology 141, 3-14
   Abstract »    Full Text »    PDF »
Auxin signaling.
T. Paciorek and J. Friml (2006)
J. Cell Sci. 119, 1199-1202
   Full Text »    PDF »
Degrade to create: developmental requirements for ubiquitin-mediated proteolysis during early C. elegans embryogenesis.
B. Bowerman and T. Kurz (2006)
Development 133, 773-784
   Abstract »    Full Text »    PDF »
Model of the TIR1 Pathway for Auxin-Mediated Gene Expression.
M. Laskowski (2006)
Sci. STKE 2006, tr1
   Abstract »    Full Text »    PDF »
A Two-dimensional Electrophoresis Proteomic Reference Map and Systematic Identification of 1367 Proteins from a Cell Suspension Culture of the Model Legume Medicago truncatula.
Z. Lei, A. M. Elmer, B. S. Watson, R. A. Dixon, P. J. Mendes, and L. W. Sumner (2005)
Mol. Cell. Proteomics 4, 1812-1825
   Abstract »    Full Text »    PDF »
The csnD/csnE Signalosome Genes Are Involved in the Aspergillus nidulans DNA Damage Response.
J. F. Lima, I. Malavazi, M. R. von Zeska Kress Fagundes, M. Savoldi, M. H. S. Goldman, E. Schwier, G. H. Braus, and G. H. Goldman (2005)
Genetics 171, 1003-1015
   Abstract »    Full Text »    PDF »
A Mutation in the Anticodon of a Single tRNAala Is Sufficient to Confer Auxin Resistance in Arabidopsis.
J. Perry, X. Dai, and Y. Zhao (2005)
Plant Physiology 139, 1284-1290
   Abstract »    Full Text »    PDF »
RAC GTPases in Tobacco and Arabidopsis Mediate Auxin-Induced Formation of Proteolytically Active Nuclear Protein Bodies That Contain AUX/IAA Proteins.
L.-z. Tao, A. Y. Cheung, C. Nibau, and H.-m. Wu (2005)
PLANT CELL 17, 2369-2383
   Abstract »    Full Text »    PDF »
Loss of the CONSTITUTIVE PHOTOMORPHOGENIC9 Signalosome Subunit 5 Is Sufficient to Cause the cop/det/fus Mutant Phenotype in Arabidopsis.
E. M.N. Dohmann, C. Kuhnle, and C. Schwechheimer (2005)
PLANT CELL 17, 1967-1978
   Abstract »    Full Text »    PDF »
The COP9 signalosome regulates the Neurospora circadian clock by controlling the stability of the SCFFWD-1 complex.
Q. He, P. Cheng, Q. He, and Y. Liu (2005)
Genes & Dev. 19, 1518-1531
   Abstract »    Full Text »    PDF »
Two Homologous ATP-Binding Cassette Transporter Proteins, AtMDR1 and AtPGP1, Regulate Arabidopsis Photomorphogenesis and Root Development by Mediating Polar Auxin Transport.
R. Lin and H. Wang (2005)
Plant Physiology 138, 949-964
   Abstract »    Full Text »    PDF »
Auxin: Regulation, Action, and Interaction.
A. W. WOODWARD and B. BARTEL (2005)
Ann. Bot. 95, 707-735
   Abstract »    Full Text »    PDF »
Arabidopsis Has Two Redundant Cullin3 Proteins That Are Essential for Embryo Development and That Interact with RBX1 and BTB Proteins to Form Multisubunit E3 Ubiquitin Ligase Complexes in Vivo.
P. Figueroa, G. Gusmaroli, G. Serino, J. Habashi, L. Ma, Y. Shen, S. Feng, M. Bostick, J. Callis, H. Hellmann, et al. (2005)
PLANT CELL 17, 1180-1195
   Abstract »    Full Text »    PDF »
Light Regulates COP1-Mediated Degradation of HFR1, a Transcription Factor Essential for Light Signaling in Arabidopsis.
J. Yang, R. Lin, J. Sullivan, U. Hoecker, B. Liu, L. Xu, X. W. Deng, and H. Wang (2005)
PLANT CELL 17, 804-821
   Abstract »    Full Text »    PDF »
Identification of Genes that are Up- or Down-regulated in the Apical or Basal Cell of Maize Two-celled Embryos and Monitoring their Expression During Zygote Development by a Cell Manipulation- and PCR-based Approach.
T. Okamoto, S. Scholten, H. Lorz, and E. Kranz (2005)
Plant Cell Physiol. 46, 332-338
   Abstract »    Full Text »    PDF »
Arabidopsis AtCUL3a and AtCUL3b Form Complexes with Members of the BTB/POZ-MATH Protein Family.
H. Weber, A. Bernhardt, M. Dieterle, P. Hano, A. Mutlu, M. Estelle, P. Genschik, and H. Hellmann (2005)
Plant Physiology 137, 83-93
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome Pathway and Plant Development.
J. Moon, G. Parry, and M. Estelle (2004)
PLANT CELL 16, 3181-3195
   Full Text »    PDF »
Translational Regulation via 5' mRNA Leader Sequences Revealed by Mutational Analysis of the Arabidopsis Translation Initiation Factor Subunit eIF3h.
T.-H. Kim, B.-H. Kim, A. Yahalom, D. A. Chamovitz, and A. G. von Arnim (2004)
PLANT CELL 16, 3341-3356
   Abstract »    Full Text »    PDF »
The Arabidopsis CSN5A and CSN5B Subunits Are Present in Distinct COP9 Signalosome Complexes, and Mutations in Their JAMM Domains Exhibit Differential Dominant Negative Effects on Development.
G. Gusmaroli, S. Feng, and X. W. Deng (2004)
PLANT CELL 16, 2984-3001
   Abstract »    Full Text »    PDF »
Identification of inhibitors of auxin transcriptional activation by means of chemical genetics in Arabidopsis.
J. I. Armstrong, S. Yuan, J. M. Dale, V. N. Tanner, and A. Theologis (2004)
PNAS 101, 14978-14983
   Abstract »    Full Text »    PDF »
Multiple Functions of Jab1 Are Required for Early Embryonic Development and Growth Potential in Mice.
K. Tomoda, N. Yoneda-Kato, A. Fukumoto, S. Yamanaka, and J.-y. Kato (2004)
J. Biol. Chem. 279, 43013-43018
   Abstract »    Full Text »    PDF »
Arabidopsis COP10 forms a complex with DDB1 and DET1 in vivo and enhances the activity of ubiquitin conjugating enzymes.
Y. Yanagawa, J. A. Sullivan, S. Komatsu, G. Gusmaroli, G. Suzuki, J. Yin, T. Ishibashi, Y. Saijo, V. Rubio, S. Kimura, et al. (2004)
Genes & Dev. 18, 2172-2181
   Abstract »    Full Text »    PDF »
Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation.
S. Feng, Y. Shen, J. A. Sullivan, V. Rubio, Y. Xiong, T.-p. Sun, and X. W. Deng (2004)
PLANT CELL 16, 1870-1882
   Abstract »    Full Text »    PDF »
Arabidopsis ETA2, an Apparent Ortholog of the Human Cullin-Interacting Protein CAND1, Is Required for Auxin Responses Mediated by the SCFTIR1 Ubiquitin Ligase.
H.-w. Chuang, W. Zhang, and W. M. Gray (2004)
PLANT CELL 16, 1883-1897
   Abstract »    Full Text »    PDF »
AtCAND1, A HEAT-Repeat Protein That Participates in Auxin Signaling in Arabidopsis.
Y. Cheng, X. Dai, and Y. Zhao (2004)
Plant Physiology 135, 1020-1026
   Abstract »    Full Text »    PDF »
The Red Side of Photomorphogenesis.
B. M. Parks (2003)
Plant Physiology 133, 1437-1444
   Full Text »
Impaired Induction of the Jasmonate Pathway in the Rice Mutant hebiba.
M. Riemann, A. Muller, A. Korte, M. Furuya, E. W. Weiler, and P. Nick (2003)
Plant Physiology 133, 1820-1830
   Abstract »    Full Text »
p-Chlorophenoxyisobutyric Acid Impairs Auxin Response in Arabidopsis Root.
Y. Oono, C. Ooura, A. Rahman, E. T. Aspuria, K.-i. Hayashi, A. Tanaka, and H. Uchimiya (2003)
Plant Physiology 133, 1135-1147
   Abstract »    Full Text »    PDF »
Inositol 1,3,4-Trisphosphate 5/6-Kinase Inhibits Tumor Necrosis Factor-induced Apoptosis.
Y. Sun, Y. Mochizuki, and P. W. Majerus (2003)
J. Biol. Chem. 278, 43645-43653
   Abstract »    Full Text »    PDF »
Disruption of the COP9 Signalosome Csn2 Subunit in Mice Causes Deficient Cell Proliferation, Accumulation of p53 and Cyclin E, and Early Embryonic Death.
K. Lykke-Andersen, L. Schaefer, S. Menon, X.-W. Deng, J. B. Miller, and N. Wei (2003)
Mol. Cell. Biol. 23, 6790-6797
   Abstract »    Full Text »    PDF »
Members of the Arabidopsis-SKP1-like Gene Family Exhibit a Variety of Expression Patterns and May Play Diverse Roles in Arabidopsis.
D. Zhao, W. Ni, B. Feng, T. Han, M. G. Petrasek, and H. Ma (2003)
Plant Physiology 133, 203-217
   Abstract »    Full Text »    PDF »
DEN1 Is a Dual Function Protease Capable of Processing the C Terminus of Nedd8 and Deconjugating Hyper-neddylated CUL1.
K. Wu, K. Yamoah, G. Dolios, T. Gan-Erdene, P. Tan, A. Chen, C.-g. Lee, N. Wei, K. D. Wilkinson, R. Wang, et al. (2003)
J. Biol. Chem. 278, 28882-28891
   Abstract »    Full Text »    PDF »
Transcription Factor IIB (TFIIB)-Related Protein (pBrp), a Plant-Specific Member of the TFIIB-Related Protein Family.
T. Lagrange, M.-A. Hakimi, D. Pontier, F. Courtois, J. P. Alcaraz, D. Grunwald, E. Lam, and S. Lerbs-Mache (2003)
Mol. Cell. Biol. 23, 3274-3286
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Interacts with SCFUFO and Participates in Arabidopsis Flower Development.
X. Wang, S. Feng, N. Nakayama, W. L. Crosby, V. Irish, X. W. Deng, and N. Wei (2003)
PLANT CELL 15, 1071-1082
   Abstract »    Full Text »    PDF »
The COP9 Signalosome Interacts Physically with SCFCOI1 and Modulates Jasmonate Responses.
S. Feng, L. Ma, X. Wang, D. Xie, S. P. Dinesh-Kumar, N. Wei, and X. W. Deng (2003)
PLANT CELL 15, 1083-1094
   Abstract »    Full Text »    PDF »
Isolation of tobacco ubiquitin-conjugating enzyme cDNA in a yeast two-hybrid system with tobacco ERF3 as bait and its characterization of specific interaction.
T. Koyama, T. Okada, S. Kitajima, M. Ohme-Takagi, H. Shinshi, and F. Sato (2003)
J. Exp. Bot. 54, 1175-1181
   Abstract »    Full Text »    PDF »
Analysis of the mutational effects of the COP/DET/FUS loci on genome expression profiles reveals their overlapping yet not identical roles in regulating Arabidopsis seedling development.
L. Ma, H. Zhao, and X. W. Deng (2003)
Development 130, 969-981
   Abstract »    Full Text »    PDF »
The NEDD8 Pathway Is Required for Proteasome-Mediated Degradation of Human Estrogen Receptor (ER)-{alpha} and Essential for the Antiproliferative Activity of ICI 182,780 in ER{alpha}-Positive Breast Cancer Cells.
M. Fan, R. M. Bigsby, and K. P. Nephew (2003)
Mol. Endocrinol. 17, 356-365
   Abstract »    Full Text »    PDF »
Characterization of the Last Subunit of the Arabidopsis COP9 Signalosome.
N. A. Eckardt (2003)
PLANT CELL 15, 580-581
   Full Text »    PDF »
Characterization of the Last Subunit of the Arabidopsis COP9 Signalosome: Implications for the Overall Structure and Origin of the Complex.
G. Serino, H. Su, Z. Peng, T. Tsuge, N. Wei, H. Gu, and X. W. Deng (2003)
PLANT CELL 15, 719-731
   Abstract »    Full Text »    PDF »
Plasma Membrane H+-ATPase Is Involved in Auxin-Mediated Cell Elongation during Wheat Embryo Development.
N. Rober-Kleber, J. T.P. Albrechtova, S. Fleig, N. Huck, W. Michalke, E. Wagner, V. Speth, G. Neuhaus, and C. Fischer-Iglesias (2003)
Plant Physiology 131, 1302-1312
   Abstract »    Full Text »    PDF »
Arabidopsis E2Fc Functions in Cell Division and Is Degraded by the Ubiquitin-SCFAtSKP2 Pathway in Response to Light.
J. C. del Pozo, M. B. Boniotti, and C. Gutierrez (2002)
PLANT CELL 14, 3057-3071
   Abstract »    Full Text »    PDF »
Inositol 1,3,4-Trisphosphate 5/6-Kinase Associates with the COP9 Signalosome by Binding to CSN1.
Y. Sun, M. P. Wilson, and P. W. Majerus (2002)
J. Biol. Chem. 277, 45759-45764
   Abstract »    Full Text »    PDF »
Role of Predicted Metalloprotease Motif of Jab1/Csn5 in Cleavage of Nedd8 from Cul1.
G. A. Cope, G. S. B. Suh, L. Aravind, S. E. Schwarz, S. L. Zipursky, E. V. Koonin, and R. J. Deshaies (2002)
Science 298, 608-611
   Abstract »    Full Text »    PDF »
Multiple Ubiquitin Ligase-Mediated Processes Require COP9 Signalosome and AXR1 Function.
C. Schwechheimer, G. Serino, and X.-W. Deng (2002)
PLANT CELL 14, 2553-2563
   Abstract »    Full Text »    PDF »
Distinct protein degradation mechanisms mediated by Cul1 and Cul3 controlling Ci stability in Drosophila eye development.
C.-Y. Ou, Y.-F. Lin, Y.-J. Chen, and C.-T. Chien (2002)
Genes & Dev. 16, 2403-2414
   Abstract »    Full Text »    PDF »
Dysregulation of protein modification by ISG15 results in brain cell injury.
K. J. Ritchie, M. P. Malakhov, C. J. Hetherington, L. Zhou, M.-T. Little, O. A. Malakhova, J. C. Sipe, S. H. Orkin, and D.-E. Zhang (2002)
Genes & Dev. 16, 2207-2212
   Abstract »    Full Text »    PDF »
Role of the Arabidopsis RING-H2 Protein RBX1 in RUB Modification and SCF Function.
W. M. Gray, H. Hellmann, S. Dharmasiri, and M. Estelle (2002)
PLANT CELL 14, 2137-2144
   Abstract »    Full Text »    PDF »
Ubiquitin ligase-associated protein SGT1 is required for host and nonhost disease resistance in plants.
J. R. Peart, R. Lu, A. Sadanandom, I. Malcuit, P. Moffett, D. C. Brice, L. Schauser, D. A. W. Jaggard, S. Xiao, M. J. Coleman, et al. (2002)
PNAS 99, 10865-10869
   Abstract »    Full Text »    PDF »
The SCFCOI1 Ubiquitin-Ligase Complexes Are Required for Jasmonate Response in Arabidopsis.
L. Xu, F. Liu, E. Lechner, P. Genschik, W. L. Crosby, H. Ma, W. Peng, D. Huang, and D. Xie (2002)
PLANT CELL 14, 1919-1935
   Abstract »    Full Text »    PDF »
Role of SCF Ubiquitin-Ligase and the COP9 Signalosome in the N Gene-Mediated Resistance Response to Tobacco mosaic virus.
Y. Liu, M. Schiff, G. Serino, X.-W. Deng, and S. P. Dinesh-Kumar (2002)
PLANT CELL 14, 1483-1496
   Abstract »    Full Text »    PDF »
The NEDD8 Pathway Is Essential for SCFbeta -TrCP-mediated Ubiquitination and Processing of the NF-kappa B Precursor p105.
R. E. Amir, K. Iwai, and A. Ciechanover (2002)
J. Biol. Chem. 277, 23253-23259
   Abstract »    Full Text »    PDF »
Null Mutation of AtCUL1 Causes Arrest in Early Embryogenesis in Arabidopsis.
W.-H. Shen, Y. Parmentier, H. Hellmann, E. Lechner, A. Dong, J. Masson, F. Granier, L. Lepiniec, M. Estelle, and P. Genschik (2002)
Mol. Biol. Cell 13, 1916-1928
   Abstract »    Full Text »    PDF »
Two interacting bZIP proteins are direct targets of COP1-mediated control of light-dependent gene expression in Arabidopsis.
M. Holm, L.-G. Ma, L.-J. Qu, and X.-W. Deng (2002)
Genes & Dev. 16, 1247-1259
   Abstract »    Full Text »    PDF »
A Breakdown in Defense Signaling.
P. N. Dodds and C. Schwechheimer (2002)
PLANT CELL 14, S5-S8
   Full Text »    PDF »
Ubiquitination and Auxin Signaling: A Degrading Story.
S. Kepinski and O. Leyser (2002)
PLANT CELL 14, S81-S95
   Full Text »    PDF »
The Ubiquitin-Proteasome Proteolytic Pathway: Destruction for the Sake of Construction.
M. H. Glickman and A. Ciechanover (2002)
Physiol Rev 82, 373-428
   Abstract »    Full Text »    PDF »
PLANT BIOLOGY: Enhanced: Resisting Attack.
M. Nishimura and S. Somerville (2002)
Science 295, 2032-2033
   Abstract »    Full Text »    PDF »
The RAR1 Interactor SGT1, an Essential Component of R Gene-Triggered Disease Resistance.
C. Azevedo, A. Sadanandom, K. Kitagawa, A. Freialdenhoven, K. Shirasu, and P. Schulze-Lefert (2002)
Science 295, 2073-2076
   Abstract »    Full Text »    PDF »
Arabidopsis COP10 is a ubiquitin-conjugating enzyme variant that acts together with COP1 and the COP9 signalosome in repressing photomorphogenesis.
G. Suzuki, Y. Yanagawa, S. F. Kwok, M. Matsui, and X.-W. Deng (2002)
Genes & Dev. 16, 554-559
   Abstract »    Full Text »    PDF »
The Activating Enzyme of NEDD8 Inhibits Steroid Receptor Function.
M. Fan, X. Long, J. A. Bailey, C. A. Reed, E. Osborne, E. A. Gize, E. A. Kirk, R. M. Bigsby, and K. P. Nephew (2002)
Mol. Endocrinol. 16, 315-330
   Abstract »    Full Text »    PDF »
The COP9 signalosome: at the interface between signal transduction and ubiquitin-dependent proteolysis.
D. Bech-Otschir, M. Seeger, and W. Dubiel (2002)
J. Cell Sci. 115, 467-473
   Abstract »    Full Text »    PDF »
COP9 signalosome subunits 4 and 5 regulate multiple pleiotropic pathways in Drosophila melanogaster.
E. Oron, M. Mannervik, S. Rencus, O. Harari-Steinberg, S. Neuman-Silberberg, D. Segal, and D. A. Chamovitz (2002)
Development 129, 4399-4409
   Abstract »    Full Text »    PDF »
The Nedd8-conjugated ROC1-CUL1 Core Ubiquitin Ligase Utilizes Nedd8 Charged Surface Residues for Efficient Polyubiquitin Chain Assembly Catalyzed by Cdc34.
K. Wu, A. Chen, P. Tan, and Z.-Q. Pan (2002)
J. Biol. Chem. 277, 516-527
   Abstract »    Full Text »
Cytokinin Growth Responses in Arabidopsis Involve the 26S Proteasome Subunit RPN12.
J. Smalle, J. Kurepa, P. Yang, E. Babiychuk, S. Kushnir, A. Durski, and R. D. Vierstra (2002)
PLANT CELL 14, 17-32
   Abstract »    Full Text »    PDF »
The DELLA motif is essential for gibberellin-induced degradation of RGA.
A. Dill, H.-S. Jung, and T.-p. Sun (2001)
PNAS 98, 14162-14167
   Abstract »    Full Text »    PDF »
Molecular Characterization of Subunit 6 of the COP9 Signalosome and Its Role in Multifaceted Developmental Processes in Arabidopsis.
Z. Peng, G. Serino, and X.-W. Deng (2001)
PLANT CELL 13, 2393-2407
   Abstract »    Full Text »    PDF »
Plant Biology 2001.
N. A. Eckardt, H.-T. Cho, R. M. Perrin, and M. R. Willmann (2001)
PLANT CELL 13, 2165-2173
   Full Text »    PDF »
Rapid Degradation of Auxin/Indoleacetic Acid Proteins Requires Conserved Amino Acids of Domain II and Is Proteasome Dependent.
J. A. Ramos, N. Zenser, O. Leyser, and J. Callis (2001)
PLANT CELL 13, 2349-2360
   Abstract »    Full Text »    PDF »
Auxin modulates the degradation rate of Aux/IAA proteins.
N. Zenser, A. Ellsmore, C. Leasure, and J. Callis (2001)
PNAS 98, 11795-11800
   Abstract »    Full Text »    PDF »
Arabidopsis Research 2001.
N. A. Eckardt, T. Araki, C. Benning, P. Cubas, J. Goodrich, S. E. Jacobsen, P. Masson, E. Nambara, R. Simon, S. Somerville, et al. (2001)
PLANT CELL 13, 1973-1982
   Full Text »    PDF »
Does EID1 Aid the Fine-Tuning of Phytochrome A Signal Transduction in Arabidopsis?.
H. Okamoto, L. Qu, and X.-W. Deng (2001)
PLANT CELL 13, 1983-1986
   Full Text »    PDF »
A Hitchhiker's Guide to the Proteasome.
A. G. von Arnim (2001)
Sci. STKE 2001, pe2
   Abstract »    Full Text »    PDF »
The Cytoplasmic Shuttling and Subsequent Degradation of p27Kip1 Mediated by Jab1/CSN5 and the COP9 Signalosome Complex.
K. Tomoda, Y. Kubota, Y. Arata, S. Mori, M. Maeda, T. Tanaka, M. Yoshida, N. Yoneda-Kato, and J.-y. Kato (2002)
J. Biol. Chem. 277, 2302-2310
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882