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Science 292 (5522): 1725-1728

Copyright © 2001 by the American Association for the Advancement of Science

Structure of Complement Receptor 2 in Complex with Its C3d Ligand

Gerda Szakonyi,1* Joel M. Guthridge,2* Dawei Li,1 Kendra Young,2 V. Michael Holers,2 Xiaojiang S. Chen1dagger

Complement receptor 2 (CR2/CD21) is an important receptor that amplifies B lymphocyte activation by bridging the innate and adaptive immune systems. CR2 ligands include complement C3d and Epstein-Barr virus glycoprotein 350/220. We describe the x-ray structure of this CR2 domain in complex with C3d at 2.0 angstroms. The structure reveals extensive main chain interactions between C3d and only one short consensus repeat (SCR) of CR2 and substantial SCR side-side packing. These results provide a detailed understanding of receptor-ligand interactions in this protein family and reveal potential target sites for molecular drug design.

1 Department of Biochemistry and Molecular Genetics,
2 Departments of Medicine and Immunology, University of Colorado Health Science Center, School of Medicine, Denver, CO 80262, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: Xiaojiang.Chen{at}uchsc.edu


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