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Science 292 (5525): 2333-2337

Copyright © 2001 by the American Association for the Advancement of Science

Recruitment of HAT Complexes by Direct Activator Interactions with the ATM-Related Tra1 Subunit

Christine E. Brown,12 LeAnn Howe,12 Kyle Sousa,12 Stephen C. Alley,3 Michael J. Carrozza,12 Song Tan,2 Jerry L. Workman1*

Promoter-specific recruitment of histone acetyltransferase activity is often critical for transcriptional activation. We present a detailed study of the interaction between the histone acetyltransferase complexes SAGA and NuA4, and transcription activators. We demonstrate by affinity chromatography and photo-cross-linking label transfer that acidic activators directly interact with Tra1p, a shared subunit of SAGA and NuA4. Mutations within the COOH-terminus of Tra1p disrupted its interaction with activators and resulted in gene-specific transcriptional defects that correlated with lowered promoter-specific histone acetylation. These data demonstrate that the essential Tra1 protein serves as a common target for activators in both SAGA and NuA4 acetyltransferases.

1 Howard Hughes Medical Institute,
2 Department of Biochemistry and Molecular Biology,
3 Department of Chemistry, The Pennsylvania State University, 306 Althouse Laboratory, University Park, PA 16802, USA.
*   To whom correspondence should be addressed: E-mail: jlw10{at}

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Gene activation by interaction of an inhibitor with a cytoplasmic signaling protein.
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A target essential for the activity of a nonacidic yeast transcriptional activator.
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Role of the Ada2 and Ada3 Transcriptional Coactivators in Histone Acetylation.
R. Balasubramanian, M. G. Pray-Grant, W. Selleck, P. A. Grant, and S. Tan (2002)
J. Biol. Chem. 277, 7989-7995
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The SANT Domain of Ada2 Is Required for Normal Acetylation of Histones by the Yeast SAGA Complex.
D. E. Sterner, X. Wang, M. H. Bloom, G. M. Simon, and S. L. Berger (2002)
J. Biol. Chem. 277, 8178-8186
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Role of an ING1 Growth Regulator in Transcriptional Activation and Targeted Histone Acetylation by the NuA4 Complex.
A. Nourani, Y. Doyon, R. T. Utley, S. Allard, W. S. Lane, and J. Cote (2001)
Mol. Cell. Biol. 21, 7629-7640
   Abstract »    Full Text »    PDF »
The S. cerevisiae SAGA complex functions in vivo as a coactivator for transcriptional activation by Gal4.
E. Larschan and F. Winston (2001)
Genes & Dev. 15, 1946-1956
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