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Science 293 (5535): 1629-1633

Copyright © 2001 by the American Association for the Advancement of Science

Patterns of Gene Expression During Drosophila Mesoderm Development

Eileen E. M. Furlong,1 Erik C. Andersen,1* Brian Null,1 Kevin P. White,2dagger Matthew P. Scott1ddagger

The transcription factor Twist initiates Drosophila mesoderm development, resulting in the formation of heart, somatic muscle, and other cell types. Using a Drosophila embryo sorter, we isolated enough homozygous twist mutant embryos to perform DNA microarray experiments. Transcription profiles of twist loss-of-function embryos, embryos with ubiquitous twist expression, and wild-type embryos were compared at different developmental stages. The results implicate hundreds of genes, many with vertebrate homologs, in stage-specific processes in mesoderm development. One such gene, gleeful, related to the vertebrate Gli genes, is essential for somatic muscle development and sufficient to cause neural cells to express a muscle marker.

1 Departments of Developmental Biology and Genetics, Howard Hughes Medical Institute, Beckman Center B300,
2 Department of Biochemistry, 279 Campus Drive, Stanford University School of Medicine, Stanford, CA 94305-5329, USA.
*   Present address: Mail Stop 68-425 Department of Biology, Massachusetts Avenue, Cambridge, MA 02139, USA

dagger    Present address: Department of Genetics, Sterling Hall of Medicine, 333 Cedar Street, Yale University School of Medicine, New Haven, CT 06520, USA.

ddagger    To whom correspondence should be addressed. E-mail: scott{at}cmgm.stanford.edu

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