Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 293 (5535): 1651-1653

Copyright © 2001 by the American Association for the Advancement of Science

Regulation of Transcriptional Activation Domain Function by Ubiquitin

Simone E. Salghetti,1 Amy A. Caudy,12 Joshua G. Chenoweth,3 William P. Tansey1*

The ability of transcriptional activation domains (TADs) to signal ubiquitin-mediated proteolysis suggests an involvement of the ubiquitin-proteasome pathway in transcription. To probe this involvement, we asked how ubiquitylation regulates the activity of a transcription factor containing the VP16 TAD. We show that the VP16 TAD signals ubiquitylation through the Met30 ubiquitin-ligase and that Met30 is also required for the VP16 TAD to activate transcription. The requirement for Met30 in transcription is circumvented by fusion of ubiquitin to the VP16 activator, demonstrating that activator ubiquitylation is essential for transcriptional activation. We propose that ubiquitylation regulates TAD function by serving as a dual signal for activation and activator destruction.

1 Cold Spring Harbor Laboratory, 1 Bungtown Road, Post Office Box 100, Cold Spring Harbor, NY 11724, USA.
2 Watson School of Biological Sciences, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.
3 Graduate Program in Molecular and Cellular Biology, State University of New York at Stony Brook, Stony Brook, NY 11794, USA.
*   To whom correspondence should be addressed. E-mail: tansey{at}cshl.org

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mediator Recruitment to Heat Shock Genes Requires Dual Hsf1 Activation Domains and Mediator Tail Subunits Med15 and Med16.
S. Kim and D. S. Gross (2013)
J. Biol. Chem. 288, 12197-12213
   Abstract »    Full Text »    PDF »
Ubiquitination of Retinoblastoma Family Protein 1 Potentiates Gene-specific Repression Function.
N. Raj, L. Zhang, Y. Wei, D. N. Arnosti, and R. W. Henry (2012)
J. Biol. Chem. 287, 41835-41843
   Abstract »    Full Text »    PDF »
CLOCK deubiquitylation by USP8 inhibits CLK/CYC transcription in Drosophila.
W. Luo, Y. Li, C.-H. A. Tang, K. C. Abruzzi, J. Rodriguez, S. Pescatore, and M. Rosbash (2012)
Genes & Dev. 26, 2536-2549
   Abstract »    Full Text »    PDF »
Proteasome-Mediated Turnover of Arabidopsis MED25 Is Coupled to the Activation of FLOWERING LOCUS T Transcription.
S. Inigo, A. N. Giraldez, J. Chory, and P. D. Cerdan (2012)
Plant Physiology 160, 1662-1673
   Abstract »    Full Text »    PDF »
The Ubiquitin-Proteasome System of Saccharomyces cerevisiae.
D. Finley, H. D. Ulrich, T. Sommer, and P. Kaiser (2012)
Genetics 192, 319-360
   Abstract »    Full Text »    PDF »
ER{alpha} Phosphorylation at Y537 by Src Triggers E6-AP-ER{alpha} Binding, ER{alpha} Ubiquitylation, Promoter Occupancy, and Target Gene Expression.
J. Sun, W. Zhou, K. Kaliappan, Z. Nawaz, and J. M. Slingerland (2012)
Mol. Endocrinol. 26, 1567-1577
   Abstract »    Full Text »    PDF »
De Novo Assembly of the Manila Clam Ruditapes philippinarum Transcriptome Provides New Insights into Expression Bias, Mitochondrial Doubly Uniparental Inheritance and Sex Determination.
F. Ghiselli, L. Milani, P. L. Chang, D. Hedgecock, J. P. Davis, S. V. Nuzhdin, and M. Passamonti (2012)
Mol. Biol. Evol. 29, 771-786
   Abstract »    Full Text »    PDF »
Mannose receptor polyubiquitination regulates endosomal recruitment of p97 and cytosolic antigen translocation for cross-presentation.
M. Zehner, A. I. Chasan, V. Schuette, M. Embgenbroich, T. Quast, W. Kolanus, and S. Burgdorf (2011)
PNAS 108, 9933-9938
   Abstract »    Full Text »    PDF »
Ligand-Dependent Degradation of SRC-1 Is Pivotal for Progesterone Receptor Transcriptional Activity.
L. Amazit, A. Roseau, J. A. Khan, A. Chauchereau, R. K. Tyagi, H. Loosfelt, P. Leclerc, M. Lombes, and A. Guiochon-Mantel (2011)
Mol. Endocrinol. 25, 394-408
   Abstract »    Full Text »    PDF »
Paradoxical Instability-Activity Relationship Defines a Novel Regulatory Pathway for Retinoblastoma Proteins.
P. Acharya, N. Raj, M. S. Buckley, L. Zhang, S. Duperon, G. Williams, R. W. Henry, and D. N. Arnosti (2010)
Mol. Biol. Cell 21, 3890-3901
   Abstract »    Full Text »    PDF »
Epstein-Barr Virus LF2 Protein Regulates Viral Replication by Altering Rta Subcellular Localization.
A. M. F. Heilmann, M. A. Calderwood, and E. Johannsen (2010)
J. Virol. 84, 9920-9931
   Abstract »    Full Text »    PDF »
Female-specific flightless phenotype for mosquito control.
G. Fu, R. S. Lees, D. Nimmo, D. Aw, L. Jin, P. Gray, T. U. Berendonk, H. White-Cooper, S. Scaife, H. Kim Phuc, et al. (2010)
PNAS 107, 4550-4554
   Abstract »    Full Text »    PDF »
Noise Can Induce Bimodality in Positive Transcriptional Feedback Loops Without Bistability.
T.-L. To and N. Maheshri (2010)
Science 327, 1142-1145
   Abstract »    Full Text »    PDF »
No Splicing, No Dicing: Non-proteolytic Roles of the Ubiquitin-Proteasome System in Transcription.
T. Kodadek (2010)
J. Biol. Chem. 285, 2221-2226
   Abstract »    Full Text »    PDF »
Non-proteolytic Regulation of p53-mediated Transcription through Destabilization of the Activator{middle dot}Promoter Complex by the Proteasomal ATPases.
Y.-C. Kim, S.-Y. Wu, H.-S. Lim, C.-M. Chiang, and T. Kodadek (2009)
J. Biol. Chem. 284, 34522-34530
   Abstract »    Full Text »    PDF »
Cellular and Regional Distributions of Ubiquitin-Proteasome and Endocytotic Pathway Components in the Epithelium of Rat Efferent Ductules and Initial Segment of the Epididymis.
C. A. Oliveira, A. B. Victor-Costa, and R. A. Hess (2009)
30, 590-601
   Abstract »    Full Text »    PDF »
The Mdm2 Ubiquitin Ligase Enhances Transcriptional Activity of Human Papillomavirus E2.
N. Gammoh, D. Gardiol, P. Massimi, and L. Banks (2009)
J. Virol. 83, 1538-1543
   Abstract »    Full Text »    PDF »
Adenoviral E1A Function through Myc.
A. A. Chakraborty and W. P. Tansey (2009)
Cancer Res. 69, 6-9
   Abstract »    Full Text »    PDF »
Modulation of RNA polymerase II subunit composition by ubiquitylation.
A. Daulny, F. Geng, M. Muratani, J. M. Geisinger, S. E. Salghetti, and W. P. Tansey (2008)
PNAS 105, 19649-19654
   Abstract »    Full Text »    PDF »
PML Activates Transcription by Protecting HIPK2 and p300 from SCFFbx3-Mediated Degradation.
Y. Shima, T. Shima, T. Chiba, T. Irimura, P. P. Pandolfi, and I. Kitabayashi (2008)
Mol. Cell. Biol. 28, 7126-7138
   Abstract »    Full Text »    PDF »
Patterning of Inflorescences and Flowers by the F-Box Protein DOUBLE TOP and the LEAFY Homolog ABERRANT LEAF AND FLOWER of Petunia.
E. Souer, A. B. Rebocho, M. Bliek, E. Kusters, R. A.M. de Bruin, and R. Koes (2008)
PLANT CELL 20, 2033-2048
   Abstract »    Full Text »    PDF »
Activation Domain-dependent Monoubiquitylation of Gal4 Protein Is Essential for Promoter Binding in Vivo.
C. T. Archer, A. Delahodde, F. Gonzalez, S. A. Johnston, and T. Kodadek (2008)
J. Biol. Chem. 283, 12614-12623
   Abstract »    Full Text »    PDF »
CRM1-mediated Nuclear Export Is Required for 26 S Proteasome-dependent Degradation of the TRIP-Br2 Proto-oncoprotein.
J. K. Cheong, L. Gunaratnam, and S. I-H. Hsu (2008)
J. Biol. Chem. 283, 11661-11676
   Abstract »    Full Text »    PDF »
SCFCdc4 acts antagonistically to the PGC-1{alpha} transcriptional coactivator by targeting it for ubiquitin-mediated proteolysis.
B. L. Olson, M. B. Hock, S. Ekholm-Reed, J. A. Wohlschlegel, K. K. Dev, A. Kralli, and S. I. Reed (2008)
Genes & Dev. 22, 252-264
   Abstract »    Full Text »    PDF »
Dynamics of the Hypoxia-inducible Factor-1-Vascular Endothelial Growth Factor Promoter Complex.
P. Yu and T. Kodadek (2007)
J. Biol. Chem. 282, 35035-35045
   Abstract »    Full Text »    PDF »
Methylation-Acetylation Interplay Activates p53 in Response to DNA Damage.
G. S. Ivanov, T. Ivanova, J. Kurash, A. Ivanov, S. Chuikov, F. Gizatullin, E. M. Herrera-Medina, F. Rauscher III, D. Reinberg, and N. A. Barlev (2007)
Mol. Cell. Biol. 27, 6756-6769
   Abstract »    Full Text »    PDF »
Intramolecular Control of Protein Stability, Subnuclear Compartmentalization, and Coactivator Function of Peroxisome Proliferator-activated Receptor {gamma} Coactivator 1{alpha}.
M. Sano, S. Tokudome, N. Shimizu, N. Yoshikawa, C. Ogawa, K. Shirakawa, J. Endo, T. Katayama, S. Yuasa, M. Ieda, et al. (2007)
J. Biol. Chem. 282, 25970-25980
   Abstract »    Full Text »    PDF »
Ubiquitin, Hormones and Biotic Stress in Plants.
K. Dreher and J. Callis (2007)
Ann. Bot. 99, 787-822
   Abstract »    Full Text »    PDF »
Altered Promoter Recycling Rates Contribute to Dominant-Negative Activity of Human Peroxisome Proliferator-Activated Receptor-{gamma} Mutations Associated with Diabetes.
G. Li and T. Leff (2007)
Mol. Endocrinol. 21, 857-864
   Abstract »    Full Text »    PDF »
The role of the proteasomal ATPases and activator monoubiquitylation in regulating Gal4 binding to promoters.
A. Ferdous, D. Sikder, T. Gillette, K. Nalley, T. Kodadek, and S. A. Johnston (2007)
Genes & Dev. 21, 112-123
   Abstract »    Full Text »    PDF »
Sequential Modifications in Class II Transactivator Isoform 1 Induced by Lipopolysaccharide Stimulate Major Histocompatibility Complex Class II Transcription in Macrophages.
G. Drozina, J. Kohoutek, T. Nishiya, and B. M. Peterlin (2006)
J. Biol. Chem. 281, 39963-39970
   Abstract »    Full Text »    PDF »
Progesterone Receptors (PR)-B and -A Regulate Transcription by Different Mechanisms: AF-3 Exerts Regulatory Control over Coactivator Binding to PR-B.
L. Tung, H. Abdel-Hafiz, T. Shen, D. M. E. Harvell, L. K. Nitao, J. K. Richer, C. A. Sartorius, G. S. Takimoto, and K. B. Horwitz (2006)
Mol. Endocrinol. 20, 2656-2670
   Abstract »    Full Text »    PDF »
BMAL1 Shuttling Controls Transactivation and Degradation of the CLOCK/BMAL1 Heterodimer.
I. Kwon, J. Lee, S. H. Chang, N. C. Jung, B. J. Lee, G. H. Son, K. Kim, and K. H. Lee (2006)
Mol. Cell. Biol. 26, 7318-7330
   Abstract »    Full Text »    PDF »
Lives and Times of Nuclear Receptors.
E. T. Alarid (2006)
Mol. Endocrinol. 20, 1972-1981
   Abstract »    Full Text »    PDF »
Phosphorylation and Ubiquitination of the Transcription Factor Sterol Regulatory Element-binding Protein-1 in Response to DNA Binding.
T. Punga, M. T. Bengoechea-Alonso, and J. Ericsson (2006)
J. Biol. Chem. 281, 25278-25286
   Abstract »    Full Text »    PDF »
Proteasome-dependent down-regulation of activated Stat5A in the nucleus.
Y. Chen, X. Dai, A. L. Haas, R. Wen, and D. Wang (2006)
Blood 108, 566-574
   Abstract »    Full Text »    PDF »
Determinants of the Ubiquitin-mediated Degradation of the Met4 Transcription Factor.
A. Menant, P. Baudouin-Cornu, C. Peyraud, M. Tyers, and D. Thomas (2006)
J. Biol. Chem. 281, 11744-11754
   Abstract »    Full Text »    PDF »
The ETS Protein MEF Is Regulated by Phosphorylation-Dependent Proteolysis via the Protein-Ubiquitin Ligase SCFSkp2.
Y. Liu, C. V. Hedvat, S. Mao, X.-H. Zhu, J. Yao, H. Nguyen, A. Koff, and S. D. Nimer (2006)
Mol. Cell. Biol. 26, 3114-3123
   Abstract »    Full Text »    PDF »
Histone sumoylation is a negative regulator in Saccharomyces cerevisiae and shows dynamic interplay with positive-acting histone modifications.
D. Nathan, K. Ingvarsdottir, D. E. Sterner, G. R. Bylebyl, M. Dokmanovic, J. A. Dorsey, K. A. Whelan, M. Krsmanovic, W. S. Lane, P. B. Meluh, et al. (2006)
Genes & Dev. 20, 966-976
   Abstract »    Full Text »    PDF »
NXP-2 association with SUMO-2 depends on lysines required for transcriptional repression.
A. Rosendorff, S. Sakakibara, S. Lu, E. Kieff, Y. Xuan, A. DiBacco, Y. Shi, Y. Shi, and G. Gill (2006)
PNAS 103, 5308-5313
   Abstract »    Full Text »    PDF »
The proteasomal ATPase complex is required for stress-induced transcription in yeast.
R. Sulahian, S. A. Johnston, and T. Kodadek (2006)
Nucleic Acids Res. 34, 1351-1357
   Abstract »    Full Text »    PDF »
Sumoylation Inhibits Cleavage of Sp1 N-terminal Negative Regulatory Domain and Inhibits Sp1-dependent Transcription.
M. L. Spengler and M. G. Brattain (2006)
J. Biol. Chem. 281, 5567-5574
   Abstract »    Full Text »    PDF »
Molecular Linkage Between the Kinase ATM and NF-{kappa}B Signaling in Response to Genotoxic Stimuli..
Z.-H. Wu, Y. Shi, R. S. Tibbetts, and S. Miyamoto (2006)
Science 311, 1141-1146
   Abstract »    Full Text »    PDF »
Overexpression of the Coactivator Bridge-1 Results in Insulin Deficiency and Diabetes.
J. L. Volinic, J. H. Lee, K. Eto, V. Kaur, and M. K. Thomas (2006)
Mol. Endocrinol. 20, 167-182
   Abstract »    Full Text »    PDF »
IL-2 production in developing Th1 cells is regulated by heterodimerization of RelA and T-bet and requires T-bet serine residue 508.
E. S. Hwang, J.-H. Hong, and L. H. Glimcher (2005)
J. Exp. Med. 202, 1289-1300
   Abstract »    Full Text »    PDF »
Redefining the Role of the Endogenous XAP2 and C-terminal hsp70-interacting Protein on the Endogenous Ah Receptors Expressed in Mouse and Rat Cell Lines.
R. S. Pollenz and E. J. Dougherty (2005)
J. Biol. Chem. 280, 33346-33356
   Abstract »    Full Text »    PDF »
Dimerization of Doublesex Is Mediated by a Cryptic Ubiquitin-associated Domain Fold: IMPLICATIONS FOR SEX-SPECIFIC GENE REGULATION.
J. R. Bayrer, W. Zhang, and M. A. Weiss (2005)
J. Biol. Chem. 280, 32989-32996
   Abstract »    Full Text »    PDF »
Ubiquitylation of Cdk9 by Skp2 Facilitates Optimal Tat Transactivation.
M. Barboric, F. Zhang, M. Besenicar, A. Plemenitas, and B. M. Peterlin (2005)
J. Virol. 79, 11135-11141
   Abstract »    Full Text »    PDF »
Cross Talk in Hormonally Regulated Gene Transcription through Induction of Estrogen Receptor Ubiquitylation.
M. Luo, M. Koh, J. Feng, Q. Wu, and P. Melamed (2005)
Mol. Cell. Biol. 25, 7386-7398
   Abstract »    Full Text »    PDF »
Mice Lacking the UBC4-testis Gene Have a Delay in Postnatal Testis Development but Normal Spermatogenesis and Fertility.
N. Bedard, P. Hingamp, Z. Pang, A. Karaplis, C. Morales, J. Trasler, D. Cyr, C. Gagnon, and S. S. Wing (2005)
Mol. Cell. Biol. 25, 6346-6354
   Abstract »    Full Text »    PDF »
DNA-Binding and Transactivation Activities Are Essential for TAp63 Protein Degradation.
H. Ying, D. L. F. Chang, H. Zheng, F. McKeon, and Z.-X. J. Xiao (2005)
Mol. Cell. Biol. 25, 6154-6164
   Abstract »    Full Text »    PDF »
Linking the ubiquitin-proteasome pathway to chromatin remodeling/modification by nuclear receptors.
H K Kinyamu, J Chen, and T K Archer (2005)
J. Mol. Endocrinol. 34, 281-297
   Abstract »    Full Text »    PDF »
B56 Regulatory Subunit of Protein Phosphatase 2A Mediates Valproic Acid-Induced p300 Degradation.
J. Chen, J. R. St-Germain, and Q. Li (2005)
Mol. Cell. Biol. 25, 525-532
   Abstract »    Full Text »    PDF »
Phosphorylation of Progesterone Receptor Serine 400 Mediates Ligand-Independent Transcriptional Activity in Response to Activation of Cyclin-Dependent Protein Kinase 2.
L. K. Pierson-Mullany and C. A. Lange (2004)
Mol. Cell. Biol. 24, 10542-10557
   Abstract »    Full Text »    PDF »
Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import.
S. M. Plafker, K. S. Plafker, A. M. Weissman, and I. G. Macara (2004)
J. Cell Biol. 167, 649-659
   Abstract »    Full Text »    PDF »
Degradation of Promoter-bound p65/RelA Is Essential for the Prompt Termination of the Nuclear Factor {kappa}B Response.
S. Saccani, I. Marazzi, A. A. Beg, and G. Natoli (2004)
J. Exp. Med.
   Abstract »    Full Text »    PDF »
Ubiquitination of Human T-Cell Leukemia Virus Type 1 Tax Modulates Its Activity.
J.-M. Peloponese Jr., H. Iha, V. R. K. Yedavalli, A. Miyazato, Y. Li, K. Haller, M. Benkirane, and K.-T. Jeang (2004)
J. Virol. 78, 11686-11695
   Abstract »    Full Text »    PDF »
Inhibiting Proteasomal Proteolysis Sustains Estrogen Receptor-{alpha} Activation.
M. Fan, H. Nakshatri, and K. P. Nephew (2004)
Mol. Endocrinol. 18, 2603-2615
   Abstract »    Full Text »    PDF »
Ubiquitin-mediated fluorescence complementation reveals that Jun ubiquitinated by Itch/AIP4 is localized to lysosomes.
D. Fang and T. K. Kerppola (2004)
PNAS 101, 14782-14787
   Abstract »    Full Text »    PDF »
Jun Blockade of Erythropoiesis: Role for Repression of GATA-1 by HERP2.
K. E. Elagib, M. Xiao, I. M. Hussaini, L. L. Delehanty, L. A. Palmer, F. K. Racke, M. J. Birrer, G. Shanmugasundaram, M. A. McDevitt, and A. N. Goldfarb (2004)
Mol. Cell. Biol. 24, 7779-7794
   Abstract »    Full Text »    PDF »
SUMO and ubiquitin in the nucleus: different functions, similar mechanisms?.
G. Gill (2004)
Genes & Dev. 18, 2046-2059
   Abstract »    Full Text »    PDF »
Ubiquitin-Proteasome-mediated Degradation of Id1 Is Modulated by MyoD.
J. S. Trausch-Azar, J. Lingbeck, A. Ciechanover, and A. L. Schwartz (2004)
J. Biol. Chem. 279, 32614-32619
   Abstract »    Full Text »    PDF »
From The Cover: The Fbw7 tumor suppressor regulates glycogen synthase kinase 3 phosphorylation-dependent c-Myc protein degradation.
M. Welcker, A. Orian, J. Jin, J. E. Grim, J. W. Harper, R. N. Eisenman, and B. E. Clurman (2004)
PNAS 101, 9085-9090
   Abstract »    Full Text »    PDF »
Wwp2, an E3 Ubiquitin Ligase That Targets Transcription Factor Oct-4 for Ubiquitination.
H. M. Xu, B. Liao, Q. J. Zhang, B. B. Wang, H. Li, X. M. Zhong, H. Z. Sheng, Y. X. Zhao, Y. M. Zhao, and Y. Jin (2004)
J. Biol. Chem. 279, 23495-23503
   Abstract »    Full Text »    PDF »
TSG101 Interacts with Apoptosis-antagonizing Transcription Factor and Enhances Androgen Receptor-mediated Transcription by Promoting Its Monoubiquitination.
S. Burgdorf, P. Leister, and K. H. Scheidtmann (2004)
J. Biol. Chem. 279, 17524-17534
   Abstract »    Full Text »    PDF »
Physical and functional association of RNA polymerase II and the proteasome.
T. G. Gillette, F. Gonzalez, A. Delahodde, S. A. Johnston, and T. Kodadek (2004)
PNAS 101, 5904-5909
   Abstract »    Full Text »    PDF »
Rapid Glucocorticoid Receptor Exchange at a Promoter Is Coupled to Transcription and Regulated by Chaperones and Proteasomes.
D. A. Stavreva, W. G. Muller, G. L. Hager, C. L. Smith, and J. G. McNally (2004)
Mol. Cell. Biol. 24, 2682-2697
   Abstract »    Full Text »    PDF »
Identification, Mutational Analysis, and Coactivator Requirements of Two Distinct Transcriptional Activation Domains of the Saccharomyces cerevisiae Hap4 Protein.
J. L. Stebbins and S. J. Triezenberg (2004)
Eukaryot. Cell 3, 339-347
   Abstract »    Full Text »    PDF »
Evidence That C Promoter-binding Factor 1 Binding Is Required for Notch-1-mediated Repression of Activator Protein-1.
J. Chu and E. H. Bresnick (2004)
J. Biol. Chem. 279, 12337-12345
   Abstract »    Full Text »    PDF »
Making Sense of Cross-Talk between Steroid Hormone Receptors and Intracellular Signaling Pathways: Who Will Have the Last Word?.
C. A. Lange (2004)
Mol. Endocrinol. 18, 269-278
   Abstract »    Full Text »    PDF »
The Co-repressor Hairless Protects ROR{alpha} Orphan Nuclear Receptor from Proteasome-mediated Degradation.
A. N. Moraitis and V. Giguere (2003)
J. Biol. Chem. 278, 52511-52518
   Abstract »    Full Text »    PDF »
Transcription-dependent degradation controls the stability of the SREBP family of transcription factors.
A. Sundqvist and J. Ericsson (2003)
PNAS 100, 13833-13838
   Abstract »    Full Text »    PDF »
Histone sumoylation is associated with transcriptional repression.
Y. Shiio and R. N. Eisenman (2003)
PNAS 100, 13225-13230
   Abstract »    Full Text »    PDF »
Down-Regulation of Histone H2B by DNA-Dependent Protein Kinase in Response to DNA Damage through Modulation of Octamer Transcription Factor 1.
C. Schild-Poulter, A. Shih, N. C. Yarymowich, and R. J. G. Hache (2003)
Cancer Res. 63, 7197-7205
   Abstract »    Full Text »    PDF »
Transcriptional activation via sequential histone H2B ubiquitylation and deubiquitylation, mediated by SAGA-associated Ubp8.
K. W. Henry, A. Wyce, W.-S. Lo, L. J. Duggan, N.C. T. Emre, C.-F. Kao, L. Pillus, A. Shilatifard, M. A. Osley, and S. L. Berger (2003)
Genes & Dev. 17, 2648-2663
   Abstract »    Full Text »    PDF »
Changes in developmental state: demolish the old to construct the new.
W. P. Voth and D. J. Stillman (2003)
Genes & Dev. 17, 2201-2204
   Full Text »    PDF »
The intracellular domain of teneurin-2 has a nuclear function and represses zic-1-mediated transcription.
C. Bagutti, G. Forro, J. Ferralli, B. Rubin, and R. Chiquet-Ehrismann (2003)
J. Cell Sci. 116, 2957-2966
   Abstract »    Full Text »    PDF »
Nuclear Factor of Activated T Cells 2 Transactivation in Mast Cells: A NOVEL ISOFORM-SPECIFIC TRANSACTIVATION DOMAIN CONFERS UNIQUE Fc{epsilon}RI RESPONSIVENESS.
M. B. Hock and M. A. Brown (2003)
J. Biol. Chem. 278, 26695-26703
   Abstract »    Full Text »    PDF »
RING Finger Protein AO7 Supports NF-{kappa}B-mediated Transcription by Interacting with the Transactivation Domain of the p65 Subunit.
K. Asamitsu, T. Tetsuka, S. Kanazawa, and T. Okamoto (2003)
J. Biol. Chem. 278, 26879-26887
   Abstract »    Full Text »    PDF »
Phosphorylation of Kruppel-like factor 5 (KLF5/IKLF) at the CBP interaction region enhances its transactivation function.
Z. Zhang and C. T. Teng (2003)
Nucleic Acids Res. 31, 2196-2208
   Abstract »    Full Text »    PDF »
Mitogen-Activated Protein Kinase Regulates Nuclear Association of Human Progesterone Receptors.
M. Qiu, A. Olsen, E. Faivre, K. B. Horwitz, and C. A. Lange (2003)
Mol. Endocrinol. 17, 628-642
   Abstract »    Full Text »    PDF »
The ubiquitin ligase Hyperplastic discs negatively regulates hedgehog and decapentaplegic expression by independent mechanisms.
J. D. Lee, K. Amanai, A. Shearn, and J. E. Treisman (2003)
Development 129, 5697-5706
   Abstract »    Full Text »    PDF »
New perspectives on the regulation of intermembrane glycerophospholipid traffic.
D. R. Voelker (2003)
J. Lipid Res. 44, 441-449
   Abstract »    Full Text »    PDF »
Phosphatidylserine Transport to the Mitochondria Is Regulated by Ubiquitination.
M. M. Schumacher, J.-Y. Choi, and D. R. Voelker (2002)
J. Biol. Chem. 277, 51033-51042
   Abstract »    Full Text »    PDF »
Involvement of Proteasome in the Dynamic Assembly of the Androgen Receptor Transcription Complex.
Z. Kang, A. Pirskanen, O. A. Janne, and J. J. Palvimo (2002)
J. Biol. Chem. 277, 48366-48371
   Abstract »    Full Text »    PDF »
Attenuation of Glucocorticoid Signaling through Targeted Degradation of p300 via the 26S Proteasome Pathway.
Q. Li, A. Su, J. Chen, Y. A. Lefebvre, and R. J. G. Hache (2002)
Mol. Endocrinol. 16, 2819-2827
   Abstract »    Full Text »    PDF »
Histone deacetylase 6 binds polyubiquitin through its zinc finger (PAZ domain) and copurifies with deubiquitinating enzymes.
S. S. Hook, A. Orian, S. M. Cowley, and R. N. Eisenman (2002)
PNAS 99, 13425-13430
   Abstract »    Full Text »    PDF »
EDD, the Human Hyperplastic Discs Protein, Has a Role in Progesterone Receptor Coactivation and Potential Involvement in DNA Damage Response.
M. J. Henderson, A. J. Russell, S. Hird, M. Munoz, J. L. Clancy, G. M. Lehrbach, S. T. Calanni, D. A. Jans, R. L. Sutherland, and C. K. W. Watts (2002)
J. Biol. Chem. 277, 26468-26478
   Abstract »    Full Text »    PDF »
Proteomics of the Eukaryotic Transcription Machinery: Identification of Proteins Associated with Components of Yeast TFIID by Multidimensional Mass Spectrometry.
S. L. Sanders, J. Jennings, A. Canutescu, A. J. Link, and P. A. Weil (2002)
Mol. Cell. Biol. 22, 4723-4738
   Abstract »    Full Text »    PDF »
The E2 Ubiquitin Conjugase Rad6 Is Required for the ArgR/Mcm1 Repression of ARG1 Transcription.
S. D. Turner, A. R. Ricci, H. Petropoulos, J. Genereaux, I. S. Skerjanc, and C. J. Brandl (2002)
Mol. Cell. Biol. 22, 4011-4019
   Abstract »    Full Text »    PDF »
Proteasomal Inhibition Enhances Glucocorticoid Receptor Transactivation and Alters Its Subnuclear Trafficking.
B. J. Deroo, C. Rentsch, S. Sampath, J. Young, D. B. DeFranco, and T. K. Archer (2002)
Mol. Cell. Biol. 22, 4113-4123
   Abstract »    Full Text »    PDF »
Mastermind mediates chromatin-specific transcription and turnover of the Notch enhancer complex.
C. J. Fryer, E. Lamar, I. Turbachova, C. Kintner, and K. A. Jones (2002)
Genes & Dev. 16, 1397-1411
   Abstract »    Full Text »    PDF »
Coordination of ATF6-mediated Transcription and ATF6 Degradation by a Domain That Is Shared with the Viral Transcription Factor, VP16.
D. J. Thuerauf, L. E. Morrison, H. Hoover, and C. C. Glembotski (2002)
J. Biol. Chem. 277, 20734-20739
   Abstract »    Full Text »    PDF »
Emerging Roles of Ubiquitin in Transcription Regulation.
R. C. Conaway, C. S. Brower, and J. W. Conaway (2002)
Science 296, 1254-1258
   Abstract »    Full Text »    PDF »
Activation of the Murine Type II Transforming Growth Factor-beta Receptor Gene. UP-REGULATION AND FUNCTION OF THE TRANSCRIPTION FACTOR Elf-3/Ert/Esx/Ese-1.
J.-H. Kim, P. J. Wilder, J. Hou, T. Nowling, and A. Rizzino (2002)
J. Biol. Chem. 277, 17520-17530
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882