Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 294 (5543): 864-867

Copyright © 2001 by the American Association for the Advancement of Science

Single-Molecule Analysis of Chemotactic Signaling in Dictyostelium Cells

Masahiro Ueda,16* Yasushi Sako,26 Toshiki Tanaka,4dagger Peter Devreotes,5 Toshio Yanagida36

Single-molecule imaging techniques were used to reveal the binding of individual cyclic adenosine 3',5'-monophosphate molecules to heterotrimeric guanine nucleotide-binding protein coupled receptors on the surface of living Dictyostelium discoideum cells. The binding sites were uniformly distributed and diffused rapidly in the plane of the membrane. The probabilities of individual association and dissociation events were greater for receptors at the anterior end of the cell. Agonist-induced receptor phosphorylation had little effect on any of the monitored properties, whereas G protein coupling influenced the binding kinetics. These observations illustrate the dynamic properties of receptors involved in gradient sensing and suggest that these may be polarized in chemotactic cells.

1 Recognition and Formation,
2 Time's Arrow and Biosignaling, Precursory Research for Embryonic Science and Technology (PRESTO), Japan Science and Technology Corporation (JST).
3 Single Molecule Processes Project, International Cooperative Research Project (ICORP), JST, Mino, Osaka 562-0035, Japan.
4 Biomolecular Engineering Research Institute, Suita, Osaka 565-0874, Japan.
5 Department of Cell Biology, Johns Hopkins Medical Institutions, Baltimore, MD 21205, USA.
6 Department of Physiology and Biosignaling, Graduate School of Medicine, Osaka University, Suita, Osaka 565-0871, Japan.
*   To whom correspondence should be addressed. E-mail: ueda{at}

dagger    Present address: Department of Applied Chemistry, Nagoya Institute of Technology, Showa-ku, Nagoya 466-8555, Japan.

How selection affects phenotypic fluctuation.
Y. Ito, H. Toyota, K. Kaneko, and T. Yomo (2014)
Mol Syst Biol 5, 264
   Abstract »    Full Text »    PDF »
Imaging single molecules in living cells for systems biology.
Y. Sako (2014)
Mol Syst Biol 2, 56
   Abstract »    Full Text »    PDF »
Actin dynamics rapidly reset chemoattractant receptor sensitivity following adaptation in neutrophils.
S. N. Dandekar, J. S. Park, G. E. Peng, J. J. Onuffer, W. A. Lim, and O. D. Weiner (2013)
Phil Trans R Soc B 368, 20130008
   Abstract »    Full Text »    PDF »
High fidelity information processing in folic acid chemotaxis of Dictyostelium amoebae.
I. Segota, S. Mong, E. Neidich, A. Rachakonda, C. J. Lussenhop, and C. Franck (2013)
J R Soc Interface 10, 20130606
   Abstract »    Full Text »    PDF »
Molecular assembly and dynamics of fluorescent protein-tagged single KCa1.1 channel in expression system and vascular smooth muscle cells.
H. Yamamura, C. Ikeda, Y. Suzuki, S. Ohya, and Y. Imaizumi (2012)
Am J Physiol Cell Physiol 302, C1257-C1268
   Abstract »    Full Text »    PDF »
Single-Molecule Imaging of Stochastic Signaling Events in Living Cells.
S. Matsuoka, Y. Miyanaga, T. Yanagida, and M. Ueda (2012)
Cold Spring Harb Protoc 2012, pdb.top068189
   Abstract »    Full Text »    PDF »
Chemical Visualization of an Attractant Peptide, LURE.
H. Goto, S. Okuda, A. Mizukami, H. Mori, N. Sasaki, D. Kurihara, and T. Higashiyama (2011)
Plant Cell Physiol. 52, 49-58
   Abstract »    Full Text »    PDF »
Coupling Mechanism of a GPCR and a Heterotrimeric G Protein During Chemoattractant Gradient Sensing in Dictyostelium.
X. Xu, T. Meckel, J. A. Brzostowski, J. Yan, M. Meier-Schellersheim, and T. Jin (2010)
Science Signaling 3, ra71
   Abstract »    Full Text »    PDF »
Mobility of G proteins is heterogeneous and polarized during chemotaxis.
F. van Hemert, M. D. Lazova, B. E. Snaar-Jagaska, and T. Schmidt (2010)
J. Cell Sci. 123, 2922-2930
   Abstract »    Full Text »    PDF »
Self-organization of the phosphatidylinositol lipids signaling system for random cell migration.
Y. Arai, T. Shibata, S. Matsuoka, M. J. Sato, T. Yanagida, and M. Ueda (2010)
PNAS 107, 12399-12404
   Abstract »    Full Text »    PDF »
External and internal constraints on eukaryotic chemotaxis.
D. Fuller, W. Chen, M. Adler, A. Groisman, H. Levine, W.-J. Rappel, and W. F. Loomis (2010)
PNAS 107, 9656-9659
   Abstract »    Full Text »    PDF »
Direct detection of guidance receptor activity during border cell migration.
K. Janssens, H.-H. Sung, and P. Rorth (2010)
PNAS 107, 7323-7328
   Abstract »    Full Text »    PDF »
The Signaling Mechanisms Underlying Cell Polarity and Chemotaxis.
F. Wang (2009)
Cold Spring Harb Perspect Biol 1, a002980
   Abstract »    Full Text »    PDF »
Dynamic localization of G proteins in Dictyostelium discoideum.
C. A. Elzie, J. Colby, M. A. Sammons, and C. Janetopoulos (2009)
J. Cell Sci. 122, 2597-2603
   Abstract »    Full Text »    PDF »
Receptor noise limitations on chemotactic sensing.
W.-J. Rappel and H. Levine (2008)
PNAS 105, 19270-19275
   Abstract »    Full Text »    PDF »
Activation of G{alpha}i3 triggers cell migration via regulation of GIV.
P. Ghosh, M. Garcia-Marcos, S. J. Bornheimer, and M. G. Farquhar (2008)
J. Cell Biol. 182, 381-393
   Abstract »    Full Text »    PDF »
Directional control of cell motility through focal adhesion positioning and spatial control of Rac activation.
N. Xia, C. K. Thodeti, T. P. Hunt, Q. Xu, M. Ho, G. M. Whitesides, R. Westervelt, and D. E. Ingber (2008)
FASEB J 22, 1649-1659
   Abstract »    Full Text »    PDF »
A spatially restricted increase in receptor mobility is involved in directional sensing during Dictyostelium discoideum chemotaxis.
S. de Keijzer, A. Serge, F. van Hemert, P. H. M. Lommerse, G. E. M. Lamers, H. P. Spaink, T. Schmidt, and B. E. Snaar-Jagalska (2008)
J. Cell Sci. 121, 1750-1757
   Abstract »    Full Text »    PDF »
Multiple-state reactions between the epidermal growth factor receptor and Grb2 as observed by using single-molecule analysis.
M. Morimatsu, H. Takagi, K. G. Ota, R. Iwamoto, T. Yanagida, and Y. Sako (2007)
PNAS 104, 18013-18018
   Abstract »    Full Text »    PDF »
Locally controlled inhibitory mechanisms are involved in eukaryotic GPCR-mediated chemosensing.
X. Xu, M. Meier-Schellersheim, J. Yan, and T. Jin (2007)
J. Cell Biol. 178, 141-153
   Abstract »    Full Text »    PDF »
Possible roles of the endocytic cycle in cell motility.
D. Traynor and R. R. Kay (2007)
J. Cell Sci. 120, 2318-2327
   Abstract »    Full Text »    PDF »
Asymmetric redistribution of GABA receptors during GABA gradient sensing by nerve growth cones analyzed by single quantum dot imaging.
C. Bouzigues, M. Morel, A. Triller, and M. Dahan (2007)
PNAS 104, 11251-11256
   Abstract »    Full Text »    PDF »
Parsing the Motion of Single Molecules: A Novel Algorithm for Deconvoluting the Dynamics of Individual Receptors at the Cell Surface.
I. Ghosh and M. J. Wirth (2007)
Sci. STKE 2007, pe28
   Abstract »    Full Text »    PDF »
2006 Winner: Satomi Matsuoka.
F. Watt (2007)
J. Cell Sci. 120, 1503
   Full Text »    PDF »
Single-molecule analysis of epidermal growth factor binding on the surface of living cells.
Y. Teramura, J. Ichinose, H. Takagi, K. Nishida, T. Yanagida, and Y. Sako (2006)
EMBO J. 25, 4215-4222
   Abstract »    Full Text »    PDF »
Imaging of cell migration.
D. Dormann and C. J. Weijer (2006)
EMBO J. 25, 3480-3493
   Abstract »    Full Text »    PDF »
Living cells as test tubes..
X. S. Xie, J. Yu, and W. Y. Yang (2006)
Science 312, 228-230
   Abstract »    Full Text »    PDF »
Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein.
S. Matsuoka, M. Iijima, T. M. Watanabe, H. Kuwayama, T. Yanagida, P. N. Devreotes, and M. Ueda (2006)
J. Cell Sci. 119, 1071-1079
   Abstract »    Full Text »    PDF »
Tumor suppressor PTEN acts through dynamic interaction with the plasma membrane..
F. Vazquez, S. Matsuoka, W. R. Sellers, T. Yanagida, M. Ueda, and P. N. Devreotes (2006)
PNAS 103, 3633-3638
   Abstract »    Full Text »    PDF »
Trafficking of a Ligand-Receptor Complex on the Growth Cones as an Essential Step for the Uptake of Nerve Growth Factor at the Distal End of the Axon: A Single-Molecule Analysis.
T. Tani, Y. Miyamoto, K. E. Fujimori, T. Taguchi, T. Yanagida, Y. Sako, and Y. Harada (2005)
J. Neurosci. 25, 2181-2191
   Abstract »    Full Text »    PDF »
Quantitative Imaging of Single Live Cells Reveals Spatiotemporal Dynamics of Multistep Signaling Events of Chemoattractant Gradient Sensing in Dictyostelium.
X. Xu, M. Meier-Schellersheim, X. Jiao, L. E. Nelson, and T. Jin (2005)
Mol. Biol. Cell 16, 676-688
   Abstract »    Full Text »    PDF »
Noisy signal amplification in ultrasensitive signal transduction.
T. Shibata and K. Fujimoto (2005)
PNAS 102, 331-336
   Abstract »    Full Text »    PDF »
Single-Cell Microbiology: Tools, Technologies, and Applications.
B. F. Brehm-Stecher and E. A. Johnson (2004)
Microbiol. Mol. Biol. Rev. 68, 538-559
   Abstract »    Full Text »    PDF »
Localized chemical release from an artificial synapse chip.
M. C. Peterman, J. Noolandi, M. S. Blumenkranz, and H. A. Fishman (2004)
PNAS 101, 9951-9954
   Abstract »    Full Text »    PDF »
Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches.
M. Postma, J. Roelofs, J. Goedhart, H. M. Loovers, A. J. W. G. Visser, and P. J. M. Van Haastert (2004)
J. Cell Sci. 117, 2925-2935
   Abstract »    Full Text »    PDF »
Chemotaxis: signalling modules join hands at front and tail.
M. Postma, L. Bosgraaf, H. M. Loovers, and P. J. M. Van Haastert (2004)
EMBO Rep. 5, 35-40
   Abstract »    Full Text »    PDF »
On the relation between fluctuation and response in biological systems.
K. Sato, Y. Ito, T. Yomo, and K. Kaneko (2003)
PNAS 100, 14086-14090
   Abstract »    Full Text »    PDF »
Diffusion Dynamics of Glycine Receptors Revealed by Single-Quantum Dot Tracking.
M. Dahan, S. Levi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller (2003)
Science 302, 442-445
   Abstract »    Full Text »    PDF »
Leading the way: directional sensing through phosphatidylinositol 3-kinase and other signaling pathways.
S. Merlot and R. A. Firtel (2003)
J. Cell Sci. 116, 3471-3478
   Abstract »    Full Text »    PDF »
Eukaryotic Chemotaxis: Distinctions between Directional Sensing and Polarization.
P. Devreotes and C. Janetopoulos (2003)
J. Biol. Chem. 278, 20445-20448
   Abstract »    Full Text »    PDF »
Receptor-mediated Regulation of PI3Ks Confines PI(3,4,5)P3 to the Leading Edge of Chemotaxing Cells.
Y. E. Huang, M. Iijima, C. A. Parent, S. Funamoto, R. A. Firtel, and P. Devreotes (2003)
Mol. Biol. Cell 14, 1913-1922
   Abstract »    Full Text »    PDF »
Calpain regulates neutrophil chemotaxis.
M. A. Lokuta, P. A. Nuzzi, and A. Huttenlocher (2003)
PNAS 100, 4006-4011
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882