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Science 294 (5544): 1108-1111

Copyright © 2001 by the American Association for the Advancement of Science

Interaction of the Response Regulator ARR4 with Phytochrome B in Modulating Red Light Signaling

Uta Sweere,1 Klaus Eichenberg,1 Jens Lohrmann,1 Virtudes Mira-Rodado,1 Isabel Bäurle,1 Jörg Kudla,2 Ferenc Nagy,3 Eberhard Schäfer,1 Klaus Harter1*

The Arabidopsis thaliana response regulator 4, expressed in response to phytochrome B action, specifically interacts with the extreme amino-terminus of the photoreceptor. The response regulator 4 stabilizes the active Pfr form of phytochrome B in yeast and in planta, thus elevates the level of the active photoreceptor in vivo. Accordingly, transgenic Arabidopsis plants overexpressing the response regulator 4 display hypersensitivity to red light but not to light of other wavelengths. We propose that the response regulator 4 acts as an output element of a two-component system that modulates red light signaling on the level of the phytochrome B photoreceptor.

1 Institut für Biologie II / Botanik, Universität Freiburg, Schänzlestrasse 1, 79104 Freiburg, Germany.
2 Molekulare Botanik, Universität Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany.
3 Institute of Plant Biology, Biological Research Center, P.O. Box 521, 6701 Szeged, Hungary.
*   To whom correspondence should be addressed. E-mail: harterkl{at}

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