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Science 294 (5550): 2364-2368

Copyright © 2001 by the American Association for the Advancement of Science

Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants

Amy Hin Yan Tong,12 Marie Evangelista,3 Ainslie B. Parsons,12 Hong Xu,12 Gary D. Bader,45 Nicholas Pagé,6 Mark Robinson,1 Sasan Raghibizadeh,7 Christopher W. V. Hogue,45 Howard Bussey,6 Brenda Andrews,2* Mike Tyers,25* Charles Boone123*

In Saccharomyces cerevisiae, more than 80% of the ~6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ~4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization (BNI1, ARP2, ARC40, BIM1), DNA synthesis and repair (SGS1, RAD27), or uncharacterized functions (BBC1, NBP2) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.

1 Banting and Best Department of Medical Research, University of Toronto, Toronto ON, Canada M5G 1L6.
2 Department of Medical Genetics and Microbiology, University of Toronto, Toronto ON, Canada M5S 1A8.
3 Biology Department, Queens University, Kingston ON, Canada K7L 3N6.
4 Department of Biochemistry, University of Toronto, Toronto ON, Canada M5S 1A8.
5 Program in Molecular Biology and Cancer, Samuel Lunenfeld Research Institute, Mt. Sinai Hospital, Toronto ON, Canada M5G 1X5.
6 Department of Biology, McGill University, Montreal PQ, Canada H3A 1B1.
7 Virtek Engineering Sciences, Inc. (VESI), 1 Bedford Road, Toronto ON, Canada M5R 2J7.
*   To whom correspondence should be addressed. E-mail: brenda.andrews{at}, tyers{at}, and charlie.boone{at}

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M. R. Sorenson and S. W. Stevens (2014)
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   Abstract »    Full Text »    PDF »
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M. Spitzer, E. Griffiths, K. M. Blakely, J. Wildenhain, L. Ejim, L. Rossi, G. De Pascale, J. Curak, E. Brown, M. Tyers, et al. (2014)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. Zheng, J. J. Benschop, M. Shales, P. Kemmeren, J. Greenblatt, G. Cagney, F. Holstege, H. Li, and N. J. Krogan (2014)
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   Abstract »    Full Text »    PDF »
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B. VanderSluis, J. Bellay, G. Musso, M. Costanzo, B. Papp, F. J. Vizeacoumar, A. Baryshnikova, B. Andrews, C. Boone, and C. L. Myers (2014)
Mol Syst Biol 6, 429
   Abstract »    Full Text »    PDF »
Predicting synthetic rescues in metabolic networks.
A. E. Motter, N. Gulbahce, E. Almaas, and A.-L. Barabasi (2014)
Mol Syst Biol 4, 168
   Abstract »    Full Text »    PDF »
Metabolic networks in motion: 13C-based flux analysis.
U. Sauer (2014)
Mol Syst Biol 2, 62
   Abstract »    Full Text »    PDF »
Networks from drug-drug surfaces.
P. Yeh and R. Kishony (2014)
Mol Syst Biol 3, 85
   Full Text »    PDF »
Backup without redundancy: genetic interactions reveal the cost of duplicate gene loss.
J. Ihmels, S. R. Collins, M. Schuldiner, N. J. Krogan, and J. S. Weissman (2014)
Mol Syst Biol 3, 86
   Abstract »    Full Text »    PDF »
Pathway redundancy and protein essentiality revealed in the Saccharomyces cerevisiae interaction networks.
I. Ulitsky and R. Shamir (2014)
Mol Syst Biol 3, 104
   Abstract »    Full Text »    PDF »
Genome-wide transcriptional plasticity underlies cellular adaptation to novel challenge.
S. Stern, T. Dror, E. Stolovicki, N. Brenner, and E. Braun (2014)
Mol Syst Biol 3, 106
   Abstract »    Full Text »    PDF »
PhosphoPep--a phosphoproteome resource for systems biology research in Drosophila Kc167 cells.
B. Bodenmiller, J. Malmstrom, B. Gerrits, D. Campbell, H. Lam, A. Schmidt, O. Rinner, L. N. Mueller, P. T. Shannon, P. G. Pedrioli, et al. (2014)
Mol Syst Biol 3, 139
   Abstract »    Full Text »    PDF »
A global view of pleiotropy and phenotypically derived gene function in yeast.
A. M. Dudley, D. M. Janse, A. Tanay, R. Shamir, and G. M. Church (2014)
Mol Syst Biol 1, 2005.0001
   Abstract »    Full Text »    PDF »
Gene function prediction from congruent synthetic lethal interactions in yeast.
P. Ye, B. D. Peyser, X. Pan, J. D. Boeke, F. A. Spencer, and J. S. Bader (2014)
Mol Syst Biol 1, 2005.0026
   Abstract »    Full Text »    PDF »
The Ddc1-Mec3-Rad17 Sliding Clamp Regulates Histone-Histone Chaperone Interactions and DNA Replication-coupled Nucleosome Assembly in Budding Yeast.
R. J. Burgess, J. Han, and Z. Zhang (2014)
J. Biol. Chem. 289, 10518-10529
   Abstract »    Full Text »    PDF »
Differential network biology.
T. Ideker and N. J. Krogan (2014)
Mol Syst Biol 8, 565
   Abstract »    Full Text »    PDF »
Structure-function analysis of the Yhc1 subunit of yeast U1 snRNP and genetic interactions of Yhc1 with Mud2, Nam8, Mud1, Tgs1, U1 snRNA, SmD3 and Prp28.
B. Schwer and S. Shuman (2014)
Nucleic Acids Res. 42, 4697-4711
   Abstract »    Full Text »    PDF »
A defect in the RNA-processing protein HNRPDL causes limb-girdle muscular dystrophy 1G (LGMD1G).
N. M. Vieira, M. S. Naslavsky, L. Licinio, F. Kok, D. Schlesinger, M. Vainzof, N. Sanchez, J. P. Kitajima, L. Gal, N. Cavacana, et al. (2014)
Hum. Mol. Genet.
   Abstract »    Full Text »    PDF »
Dissecting the energy metabolism in Mycoplasma pneumoniae through genome-scale metabolic modeling.
J. A. H. Wodke, J. Puchałka, M. Lluch-Senar, J. Marcos, E. Yus, M. Godinho, R. Gutierrez-Gallego, V. A. P. M. dos Santos, L. Serrano, E. Klipp, et al. (2014)
Mol Syst Biol 9, 653
   Abstract »    Full Text »    PDF »
Nucleotide degradation and ribose salvage in yeast.
Y.-F. Xu, F. Letisse, F. Absalan, W. Lu, E. Kuznetsova, G. Brown, A. A. Caudy, A. F. Yakunin, J. R. Broach, and J. D. Rabinowitz (2014)
Mol Syst Biol 9, 665
   Abstract »    Full Text »    PDF »
A pharmaco-epistasis strategy reveals a new cell size controlling pathway in yeast.
F. Moretto, I. Sagot, B. Daignan-Fornier, and B. Pinson (2014)
Mol Syst Biol 9, 707
   Abstract »    Full Text »    PDF »
The Yeast Ess1 Prolyl Isomerase Controls Swi6 and Whi5 Nuclear Localization.
D. Atencio, C. Barnes, T. M. Duncan, I. M. Willis, and S. D. Hanes (2014)
g3 4, 523-537
   Abstract »    Full Text »    PDF »
gitter: A Robust and Accurate Method for Quantification of Colony Sizes From Plate Images.
O. Wagih and L. Parts (2014)
g3 4, 547-552
   Abstract »    Full Text »    PDF »
A future of the model organism model.
J. Rine (2014)
Mol. Biol. Cell 25, 549-553
   Abstract »    Full Text »    PDF »
An Evaluation of High-Throughput Approaches to QTL Mapping in Saccharomyces cerevisiae.
S. Wilkening, G. Lin, E. S. Fritsch, M. M. Tekkedil, S. Anders, R. Kuehn, M. Nguyen, R. S. Aiyar, M. Proctor, N. A. Sakhanenko, et al. (2014)
Genetics 196, 853-865
   Abstract »    Full Text »    PDF »
The RAVE complex is an isoform-specific V-ATPase assembly factor in yeast.
A. M. Smardon, H. I. Diab, M. Tarsio, T. T. Diakov, N. D. Nasab, R. W. West, and P. M. Kane (2014)
Mol. Biol. Cell 25, 356-367
   Abstract »    Full Text »    PDF »
Design and Analysis of Bar-seq Experiments.
D. G. Robinson, W. Chen, J. D. Storey, and D. Gresham (2014)
g3 4, 11-18
   Abstract »    Full Text »    PDF »
Resection Activity of the Sgs1 Helicase Alters the Affinity of DNA Ends for Homologous Recombination Proteins in Saccharomyces cerevisiae.
K. A. Bernstein, E. P. Mimitou, M. J. Mihalevic, H. Chen, I. Sunjaveric, L. S. Symington, and R. Rothstein (2013)
Genetics 195, 1241-1251
   Abstract »    Full Text »    PDF »
Thymol antifungal mode of action involves telomerase inhibition.
E. Darvishi, M. Omidi, A. A. Bushehri, A. Golshani, and M. L. Smith (2013)
Med Mycol 51, 826-834
   Abstract »    Full Text »    PDF »
Synthetic Lethal Targeting of Superoxide Dismutase 1 Selectively Kills RAD54B-Deficient Colorectal Cancer Cells.
B. V. Sajesh, M. Bailey, Z. Lichtensztejn, P. Hieter, and K. J. McManus (2013)
Genetics 195, 757-767
   Abstract »    Full Text »    PDF »
The million mutation project: A new approach to genetics in Caenorhabditis elegans.
O. Thompson, M. Edgley, P. Strasbourger, S. Flibotte, B. Ewing, R. Adair, V. Au, I. Chaudhry, L. Fernando, H. Hutter, et al. (2013)
Genome Res. 23, 1749-1762
   Abstract »    Full Text »    PDF »
Functional genomics in the study of yeast cell polarity: moving in the right direction.
E. Styles, J.-Y. Youn, M. Mattiazzi Usaj, and B. Andrews (2013)
Phil Trans R Soc B 368, 20130118
   Abstract »    Full Text »    PDF »
Structures of RNA polymerase II complexes with Bye1, a chromatin-binding PHF3/DIDO homologue.
K. Kinkelin, G. G. Wozniak, S. B. Rothbart, M. Lidschreiber, B. D. Strahl, and P. Cramer (2013)
PNAS 110, 15277-15282
   Abstract »    Full Text »    PDF »
Structure-function analysis of the 5' end of yeast U1 snRNA highlights genetic interactions with the Msl5*Mud2 branchpoint-binding complex and other spliceosome assembly factors.
B. Schwer, J. Chang, and S. Shuman (2013)
Nucleic Acids Res. 41, 7485-7500
   Abstract »    Full Text »    PDF »
Integrative approaches for predicting protein function and prioritizing genes for complex phenotypes using protein interaction networks.
X. Ma, T. Chen, and F. Sun (2013)
Brief Bioinform
   Abstract »    Full Text »    PDF »
Integrated platform for genome-wide screening and construction of high-density genetic interaction maps in mammalian cells.
M. Kampmann, M. C. Bassik, and J. S. Weissman (2013)
PNAS 110, E2317-E2326
   Abstract »    Full Text »    PDF »
The Modest Beginnings of One Genome Project.
D. B. Kaback (2013)
Genetics 194, 291-299
   Abstract »    Full Text »    PDF »
Ligand-induced activation of a formin-NPF pair leads to collaborative actin nucleation.
B. R. Graziano, E. M. Jonasson, J. G. Pullen, C. J. Gould, and B. L. Goode (2013)
J. Cell Biol. 201, 595-611
   Abstract »    Full Text »    PDF »
Genomic rearrangements and the evolution of clusters of locally adaptive loci.
S. Yeaman (2013)
PNAS 110, E1743-E1751
   Abstract »    Full Text »    PDF »
Loss of Vacuolar H+-ATPase (V-ATPase) Activity in Yeast Generates an Iron Deprivation Signal That Is Moderated by Induction of the Peroxiredoxin TSA2.
H. I. Diab and P. M. Kane (2013)
J. Biol. Chem. 288, 11366-11377
   Abstract »    Full Text »    PDF »
A Whole Genome Screen for Minisatellite Stability Genes in Stationary-Phase Yeast Cells.
B. Alver, P. A. Jauert, L. Brosnan, M. O'Hehir, B. VanderSluis, C. L. Myers, and D. T. Kirkpatrick (2013)
g3 3, 741-756
   Abstract »    Full Text »    PDF »
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M. Breker, M. Gymrek, and M. Schuldiner (2013)
J. Cell Biol. 200, 839-850
   Abstract »    Full Text »    PDF »
Sexual selection hinders adaptation in experimental populations of yeast.
L. P. Reding, J. P. Swaddle, and H. A. Murphy (2013)
Biol Lett 9, 20121202
   Abstract »    Full Text »    PDF »
Dissecting Protein Function: An Efficient Protocol for Identifying Separation-of-Function Mutations That Encode Structurally Stable Proteins.
J. W. Lubin, T. Rao, E. K. Mandell, D. S. Wuttke, and V. Lundblad (2013)
Genetics 193, 715-725
   Abstract »    Full Text »    PDF »
The Contribution of Systematic Approaches to Characterizing the Proteins and Functions of the Endoplasmic Reticulum.
M. Schuldiner and J. S. Weissman (2013)
Cold Spring Harb Perspect Biol 5, a013284
   Abstract »    Full Text »    PDF »
Cell-Cycle Perturbations Suppress the Slow-Growth Defect of spt10{Delta} Mutants in Saccharomyces cerevisiae.
J. S. Chang and F. Winston (2013)
g3 3, 573-583
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Genetics Predicts Candidate Therapeutic Genetic Interactions at the Mammalian Replication Fork.
D. M. van Pel, P. C. Stirling, S. W. Minaker, P. Sipahimalani, and P. Hieter (2013)
g3 3, 273-282
   Abstract »    Full Text »    PDF »
Secretory Protein Biogenesis and Traffic in the Early Secretory Pathway.
C. K. Barlowe and E. A. Miller (2013)
Genetics 193, 383-410
   Abstract »    Full Text »    PDF »
Novel Checkpoint Pathway Organization Promotes Genome Stability in Stationary-Phase Yeast Cells.
B. Alver, M. K. Kelly, and D. T. Kirkpatrick (2013)
Mol. Cell. Biol. 33, 457-472
   Abstract »    Full Text »    PDF »
Genetic Analysis of Mps3 SUN Domain Mutants in Saccharomyces cerevisiae Reveals an Interaction with the SUN-Like Protein Slp1.
J. M. Friederichs, J. M. Gardner, C. J. Smoyer, C. R. Whetstine, M. Gogol, B. D. Slaughter, and S. L. Jaspersen (2012)
g3 2, 1703-1718
   Abstract »    Full Text »    PDF »
A fine scale phenotype-genotype virulence map of a bacterial pathogen.
T. van Opijnen and A. Camilli (2012)
Genome Res. 22, 2541-2551
   Abstract »    Full Text »    PDF »
Interactions of subunit CCT3 in the yeast chaperonin CCT/TRiC with Q/N-rich proteins revealed by high-throughput microscopy analysis.
M. Nadler-Holly, M. Breker, R. Gruber, A. Azia, M. Gymrek, M. Eisenstein, K. R. Willison, M. Schuldiner, and A. Horovitz (2012)
PNAS 109, 18833-18838
   Abstract »    Full Text »    PDF »
Biological network analysis: insights into structure and functions.
X. Ma and L. Gao (2012)
Briefings in Functional Genomics 11, 434-442
   Abstract »    Full Text »    PDF »
Regulation of the formin Bnr1 by septins anda MARK/Par1-family septin-associated kinase.
S. M. Buttery, K. Kono, E. Stokasimov, and D. Pellman (2012)
Mol. Biol. Cell 23, 4041-4053
   Abstract »    Full Text »    PDF »
Ergosterol content specifies targeting of tail-anchored proteins to mitochondrial outer membranes.
K. Krumpe, I. Frumkin, Y. Herzig, N. Rimon, C. Ozbalci, B. Brugger, D. Rapaport, and M. Schuldiner (2012)
Mol. Biol. Cell 23, 3927-3935
   Abstract »    Full Text »    PDF »
Functional Analysis With a Barcoder Yeast Gene Overexpression System.
A. C. Douglas, A. M. Smith, S. Sharifpoor, Z. Yan, T. Durbic, L. E. Heisler, A. Y. Lee, O. Ryan, H. Gottert, A. Surendra, et al. (2012)
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   Abstract »    Full Text »    PDF »
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S. Dumcke, M. Seizl, S. Etzold, N. Pirkl, D. E. Martin, P. Cramer, and A. Tresch (2012)
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   Abstract »    Full Text »    PDF »
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O. Ryan, R. S. Shapiro, C. F. Kurat, D. Mayhew, A. Baryshnikova, B. Chin, Z.-Y. Lin, M. J. Cox, F. Vizeacoumar, D. Cheung, et al. (2012)
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   Abstract »    Full Text »    PDF »
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A. M. Hegnauer, N. Hustedt, K. Shimada, B. L. Pike, M. Vogel, P. Amsler, S. M. Rubin, F. van Leeuwen, A. Guenole, H. van Attikum, et al. (2012)
EMBO J. 31, 3768-3783
   Abstract »    Full Text »    PDF »
Local control of phosphatidylinositol 4-phosphate signaling in the Golgi apparatus by Vps74 and Sac1 phosphoinositide phosphatase.
C. S. Wood, C.-S. Hung, Y.-S. Huoh, C. J. Mousley, C. J. Stefan, V. Bankaitis, K. M. Ferguson, and C. G. Burd (2012)
Mol. Biol. Cell 23, 2527-2536
   Abstract »    Full Text »    PDF »
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K. Stanger, M. Gorelik, and A. R. Davidson (2012)
J. Biol. Chem. 287, 22133-22141
   Abstract »    Full Text »    PDF »
Suppression of {alpha}-synuclein toxicity and vesicle trafficking defects by phosphorylation at S129 in yeast depends on genetic context.
V. Sancenon, S.-A. Lee, C. Patrick, J. Griffith, A. Paulino, T. F. Outeiro, F. Reggiori, E. Masliah, and P. J. Muchowski (2012)
Hum. Mol. Genet. 21, 2432-2449
   Abstract »    Full Text »    PDF »
Functional specialisation of yeast Rho1 GTP exchange factors.
S. A. Krause, M. J. Cundell, P. P. Poon, J. McGhie, G. C. Johnston, C. Price, and J. V. Gray (2012)
J. Cell Sci. 125, 2721-2731
   Abstract »    Full Text »    PDF »
Structure-function analysis and genetic interactions of the yeast branchpoint binding protein Msl5.
J. Chang, B. Schwer, and S. Shuman (2012)
Nucleic Acids Res. 40, 4539-4552
   Abstract »    Full Text »    PDF »
Evolutionary rate covariation reveals shared functionality and coexpression of genes.
N. L. Clark, E. Alani, and C. F. Aquadro (2012)
Genome Res. 22, 714-720
   Abstract »    Full Text »    PDF »
Functional wiring of the yeast kinome revealed by global analysis of genetic network motifs.
S. Sharifpoor, D. van Dyk, M. Costanzo, A. Baryshnikova, H. Friesen, A. C. Douglas, J.-Y. Youn, B. VanderSluis, C. L. Myers, B. Papp, et al. (2012)
Genome Res. 22, 791-801
   Abstract »    Full Text »    PDF »
ER Cargo Properties Specify a Requirement for COPII Coat Rigidity Mediated by Sec13p.
A. Copic, C. F. Latham, M. A. Horlbeck, J. G. D'Arcangelo, and E. A. Miller (2012)
Science 335, 1359-1362
   Abstract »    Full Text »    PDF »
A conserved role for COMA/CENP-H/I/N kinetochore proteins in the spindle checkpoint.
D. R. Matson, P. B. Demirel, P. T. Stukenberg, and D. J. Burke (2012)
Genes & Dev. 26, 542-547
   Abstract »    Full Text »    PDF »
Negative feedback confers mutational robustness in yeast transcription factor regulation.
C. M. Denby, J. H. Im, R. C. Yu, C. G. Pesce, and R. B. Brem (2012)
PNAS 109, 3874-3878
   Abstract »    Full Text »    PDF »
Gene Overexpression: Uses, Mechanisms, and Interpretation.
G. Prelich (2012)
Genetics 190, 841-854
   Abstract »    Full Text »    PDF »
Vacuolar H+-ATPase Works in Parallel with the HOG Pathway To Adapt Saccharomyces cerevisiae Cells to Osmotic Stress.
S. C. Li, T. T. Diakov, J. M. Rizzo, and P. M. Kane (2012)
Eukaryot. Cell 11, 282-291
   Abstract »    Full Text »    PDF »
Endogenous DNA replication stress results in expansion of dNTP pools and a mutator phenotype.
M. B. Davidson, Y. Katou, A. Keszthelyi, T. L. Sing, T. Xia, J. Ou, J. A. Vaisica, N. Thevakumaran, L. Marjavaara, C. L. Myers, et al. (2012)
EMBO J. 31, 895-907
   Abstract »    Full Text »    PDF »
Iron-responsive Transcription Factor Aft1 Interacts with Kinetochore Protein Iml3 and Promotes Pericentromeric Cohesin.
A. Hamza and K. Baetz (2012)
J. Biol. Chem. 287, 4139-4147
   Abstract »    Full Text »    PDF »
Yeast formin Bni1p has multiple localization regions that function in polarized growth and spindle orientation.
W. Liu, F. H. Santiago-Tirado, and A. Bretscher (2012)
Mol. Biol. Cell 23, 412-422
   Abstract »    Full Text »    PDF »
RNA Polymerase II Carboxyl-terminal Domain Phosphorylation Regulates Protein Stability of the Set2 Methyltransferase and Histone H3 Di- and Trimethylation at Lysine 36.
S. M. Fuchs, K. O. Kizer, H. Braberg, N. J. Krogan, and B. D. Strahl (2012)
J. Biol. Chem. 287, 3249-3256
   Abstract »    Full Text »    PDF »
Studying synthetic lethal interactions in the zebrafish system: insight into disease genes and mechanisms.
V. A. Hajeri and J. F. Amatruda (2012)
Dis. Model. Mech. 5, 33-37
   Abstract »    Full Text »    PDF »
Desumoylation of the Endoplasmic Reticulum Membrane VAP Family Protein Scs2 by Ulp1 and SUMO Regulation of the Inositol Synthesis Pathway.
R. Felberbaum, N. R. Wilson, D. Cheng, J. Peng, and M. Hochstrasser (2012)
Mol. Cell. Biol. 32, 64-75
   Abstract »    Full Text »    PDF »
The Regulation of Filamentous Growth in Yeast.
P. J. Cullen and G. F. Sprague Jr. (2012)
Genetics 190, 23-49
   Abstract »    Full Text »    PDF »
PhenoM: a database of morphological phenotypes caused by mutation of essential genes in Saccharomyces cerevisiae.
K. Jin, J. Li, F. S. Vizeacoumar, Z. Li, R. Min, L. Zamparo, F. J. Vizeacoumar, A. Datti, B. Andrews, C. Boone, et al. (2012)
Nucleic Acids Res. 40, D687-D694
   Abstract »    Full Text »    PDF »
Drug-Sensitive DNA Polymerase {delta} Reveals a Role for Mismatch Repair in Checkpoint Activation in Yeast.
L. J. Reha-Krantz, M. S. P. Siddique, K. Murphy, A. Tam, M. O'Carroll, S. Lou, A. Schultz, and C. Boone (2011)
Genetics 189, 1211-1224
   Abstract »    Full Text »    PDF »
Yeast: An Experimental Organism for 21st Century Biology.
D. Botstein and G. R. Fink (2011)
Genetics 189, 695-704
   Abstract »    Full Text »    PDF »
Coordinated regulation of sulfur and phospholipid metabolism reflects the importance of methylation in the growth of yeast.
M. J. Hickman, A. A. Petti, O. Ho-Shing, S. J. Silverman, R. S. McIsaac, T. A. Lee, and D. Botstein (2011)
Mol. Biol. Cell 22, 4192-4204
   Abstract »    Full Text »    PDF »
Mechanism and cellular function of Bud6 as an actin nucleation-promoting factor.
B. R. Graziano, A. G. DuPage, A. Michelot, D. Breitsprecher, J. B. Moseley, I. Sagot, L. Blanchoin, and B. L. Goode (2011)
Mol. Biol. Cell 22, 4016-4028
   Abstract »    Full Text »    PDF »
A mitochondrial-focused genetic interaction map reveals a scaffold-like complex required for inner membrane organization in mitochondria.
S. Hoppins, S. R. Collins, A. Cassidy-Stone, E. Hummel, R. M. DeVay, L. L. Lackner, B. Westermann, M. Schuldiner, J. S. Weissman, and J. Nunnari (2011)
J. Cell Biol. 195, 323-340
   Abstract »    Full Text »    PDF »
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J. Usher, V. Balderas-Hernandez, P. Quon, N. D. Gold, V. J. J. Martin, R. Mahadevan, K. Baetz, and C. Nislow (2011)
g3 1, 247-258
   Abstract »    Full Text »    PDF »
Fine-tuning of the Msn2/4-mediated yeast stress responses as revealed by systematic deletion of Msn2/4 partners.
A. Sadeh, N. Movshovich, M. Volokh, L. Gheber, and A. Aharoni (2011)
Mol. Biol. Cell 22, 3127-3138
   Abstract »    Full Text »    PDF »
Control of Ubp3 ubiquitin protease activity by the Hog1 SAPK modulates transcription upon osmostress.
C. Sole, M. Nadal-Ribelles, C. Kraft, M. Peter, F. Posas, and E. de Nadal (2011)
EMBO J. 30, 3274-3284
   Abstract »    Full Text »    PDF »
A Global Perspective of the Genetic Basis for Carbonyl Stress Resistance.
S. Hoon, M. Gebbia, M. Costanzo, R. W. Davis, G. Giaever, C. Nislow, and R. Sclafani (2011)
g3 1, 219-231
   Abstract »    Full Text »    PDF »
Drosophila RNAi screening in a postgenomic world.
C. Bakal (2011)
Briefings in Functional Genomics
   Abstract »    Full Text »    PDF »
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A. B. Munkacsi, F. W. Chen, M. A. Brinkman, K. Higaki, G. D. Gutierrez, J. Chaudhari, J. V. Layer, A. Tong, M. Bard, C. Boone, et al. (2011)
J. Biol. Chem. 286, 23842-23851
   Abstract »    Full Text »    PDF »
Discovering the Targets of Drugs Via Computational Systems Biology.
H. N. Chua and F. P. Roth (2011)
J. Biol. Chem. 286, 23653-23658
   Abstract »    Full Text »    PDF »
The endocytic adaptor proteins of pathogenic fungi: charting new and familiar pathways.
P. Wang and G. Shen (2011)
Med Mycol 49, 449-457
   Abstract »    Full Text »    PDF »

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