Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Logo for

Science 295 (5553): 321-324

Copyright © 2002 by the American Association for the Advancement of Science

A Combined Experimental and Computational Strategy to Define Protein Interaction Networks for Peptide Recognition Modules

Amy Hin Yan Tong,1* Becky Drees,2* Giuliano Nardelli,3* Gary D. Bader,4* Barbara Brannetti,3 Luisa Castagnoli,3 Marie Evangelista,5 Silvia Ferracuti,3 Bryce Nelson,5 Serena Paoluzi,3 Michele Quondam,3 Adriana Zucconi,3 Christopher W. V. Hogue,4 Stanley Fields,26dagger Charles Boone,15dagger Gianni Cesareni3dagger

Peptide recognition modules mediate many protein-protein interactions critical for the assembly of macromolecular complexes. Complete genome sequences have revealed thousands of these domains, requiring improved methods for identifying their physiologically relevant binding partners. We have developed a strategy combining computational prediction of interactions from phage-display ligand consensus sequences with large-scale two-hybrid physical interaction tests. Application to yeast SH3 domains generated a phage-display network containing 394 interactions among 206 proteins and a two-hybrid network containing 233 interactions among 145 proteins. Graph theoretic analysis identified 59 highly likely interactions common to both networks. Las17 (Bee1), a member of the Wiskott-Aldrich Syndrome protein (WASP) family of actin-assembly proteins, showed multiple SH3 interactions, many of which were confirmed in vivo by coimmunoprecipitation.

1 Banting and Best Department of Medical Research and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada M5G 1L6.
2 Department of Genome Sciences and Department of Medicine, University of Washington, Box 357730, Seattle, WA 98195, USA.
3 Department of Biology, University of Rome Tor Vergata, Via Della Ricerca Scientifica, 00133 Rome, Italy.
4 Samuel Lunenfeld Research Institute, Mount Sinai Hospital, 600 University Avenue, Toronto, Ontario, Canada M5S 1A8 and Department of Biochemistry, University of Toronto, Ontario, Canada M5G 1L6.
5 Department of Biology, Queens University, Kingston, Ontario, Canada K7L 3N6.
6 Howard Hughes Medical Institute, University of Washington, Box 353360, Seattle, WA 98195, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: fields{at}, charlie.boone{at}, giovanni.cesareni{at}

The multiple-specificity landscape of modular peptide recognition domains.
D. Gfeller, F. Butty, M. Wierzbicka, E. Verschueren, P. Vanhee, H. Huang, A. Ernst, N. Dar, I. Stagljar, L. Serrano, et al. (2014)
Mol Syst Biol 7, 484
   Abstract »    Full Text »    PDF »
Characterizing WW Domain Interactions of Tumor Suppressor WWOX Reveals Its Association with Multiprotein Networks.
M. Abu-Odeh, T. Bar-Mag, H. Huang, T. Kim, Z. Salah, S. K. Abdeen, M. Sudol, D. Reichmann, S. Sidhu, P. M. Kim, et al. (2014)
J. Biol. Chem. 289, 8865-8880
   Abstract »    Full Text »    PDF »
SH3 interactome conserves general function over specific form.
X. Xin, D. Gfeller, J. Cheng, R. Tonikian, L. Sun, A. Guo, L. Lopez, A. Pavlenco, A. Akintobi, Y. Zhang, et al. (2014)
Mol Syst Biol 9, 652
   Abstract »    Full Text »    PDF »
PAH1-encoded Phosphatidate Phosphatase Plays a Role in the Growth Phase- and Inositol-mediated Regulation of Lipid Synthesis in Saccharomyces cerevisiae.
F. Pascual, A. Soto-Cardalda, and G. M. Carman (2013)
J. Biol. Chem. 288, 35781-35792
   Abstract »    Full Text »    PDF »
Searching for repetitions in biological networks: methods, resources and tools.
S. Panni and S. E. Rombo (2013)
Brief Bioinform
   Abstract »    Full Text »    PDF »
The Role of Djp1 in Import of the Mitochondrial Protein Mim1 Demonstrates Specificity between a Cochaperone and Its Substrate Protein.
D. Papic, Y. Elbaz-Alon, S. N. Koerdt, K. Leopold, D. Worm, M. Jung, M. Schuldiner, and D. Rapaport (2013)
Mol. Cell. Biol. 33, 4083-4094
   Abstract »    Full Text »    PDF »
Characterization of the Yeast Actin Patch Protein App1p Phosphatidate Phosphatase.
M. Chae and G. M. Carman (2013)
J. Biol. Chem. 288, 6427-6437
   Abstract »    Full Text »    PDF »
The Saccharomyces cerevisiae Actin Patch Protein App1p Is a Phosphatidate Phosphatase Enzyme.
M. Chae, G.-S. Han, and G. M. Carman (2012)
J. Biol. Chem. 287, 40186-40196
   Abstract »    Full Text »    PDF »
SLAC, a complex between Sla1 and Las17, regulates actin polymerization during clathrin-mediated endocytosis.
D. Feliciano and S. M. Di Pietro (2012)
Mol. Biol. Cell 23, 4256-4272
   Abstract »    Full Text »    PDF »
Charting the Landscape of Tandem BRCT Domain-Mediated Protein Interactions.
N. T. Woods, R. D. Mesquita, M. Sweet, M. A. Carvalho, X. Li, Y. Liu, H. Nguyen, C. E. Thomas, E. S. Iversen Jr., S. Marsillac, et al. (2012)
Science Signaling 5, rs6
   Abstract »    Full Text »    PDF »
PepSite: prediction of peptide-binding sites from protein surfaces.
L. G. Trabuco, S. Lise, E. Petsalaki, and R. B. Russell (2012)
Nucleic Acids Res. 40, W423-W427
   Abstract »    Full Text »    PDF »
Distinct Peptide Binding Specificities of Src Homology 3 (SH3) Protein Domains Can Be Determined by Modulation of Local Energetics across the Binding Interface.
M. Gorelik and A. R. Davidson (2012)
J. Biol. Chem. 287, 9168-9177
   Abstract »    Full Text »    PDF »
MimoDB 2.0: a mimotope database and beyond.
J. Huang, B. Ru, P. Zhu, F. Nie, J. Yang, X. Wang, P. Dai, H. Lin, F.-B. Guo, and N. Rao (2012)
Nucleic Acids Res. 40, D271-D277
   Abstract »    Full Text »    PDF »
A Conserved Residue in the Yeast Bem1p SH3 Domain Maintains the High Level of Binding Specificity Required for Function.
M. Gorelik, K. Stanger, and A. R. Davidson (2011)
J. Biol. Chem. 286, 19470-19477
   Abstract »    Full Text »    PDF »
A regression framework incorporating quantitative and negative interaction data improves quantitative prediction of PDZ domain-peptide interaction from primary sequence.
X. Shao, C. S. H. Tan, C. Voss, S. S. C. Li, N. Deng, and G. D. Bader (2011)
Bioinformatics 27, 383-390
   Abstract »    Full Text »    PDF »
A constraint network of interactions: protein-protein interaction analysis of the yeast type II phosphatase Ptc1p and its adaptor protein Nbp2p.
A. Hruby, M. Zapatka, S. Heucke, L. Rieger, Y. Wu, U. Nussbaumer, S. Timmermann, A. Dunkler, and N. Johnsson (2011)
J. Cell Sci. 124, 35-46
   Abstract »    Full Text »    PDF »
Calmodulin dissociation regulates Myo5 recruitment and function at endocytic sites.
H. Grotsch, J. P. Giblin, F.-Z. Idrissi, I.-M. Fernandez-Golbano, J. R. Collette, T. M. Newpher, V. Robles, S. K. Lemmon, and M.-I. Geli (2010)
EMBO J. 29, 2899-2914
   Abstract »    Full Text »    PDF »
Building Macromolecular Assemblies by Information-driven Docking: INTRODUCING THE HADDOCK MULTIBODY DOCKING SERVER.
E. Karaca, A. S. J. Melquiond, S. J. de Vries, P. L. Kastritis, and A. M. J. J. Bonvin (2010)
Mol. Cell. Proteomics 9, 1784-1794
   Abstract »    Full Text »    PDF »
DrugScorePPI webserver: fast and accurate in silico alanine scanning for scoring protein-protein interactions.
D. M. Kruger and H. Gohlke (2010)
Nucleic Acids Res. 38, W480-W486
   Abstract »    Full Text »    PDF »
Comparative Transcriptomic and Proteomic Profiling of Industrial Wine Yeast Strains.
D. Rossouw, A. H. van den Dool, D. Jacobson, and F. F. Bauer (2010)
Appl. Envir. Microbiol. 76, 3911-3923
   Abstract »    Full Text »    PDF »
Protein complex prediction based on simultaneous protein interaction network.
S. H. Jung, B. Hyun, W.-H. Jang, H.-Y. Hur, and D.-S. Han (2010)
Bioinformatics 26, 385-391
   Abstract »    Full Text »    PDF »
Structural, Functional, and Bioinformatic Studies Demonstrate the Crucial Role of an Extended Peptide Binding Site for the SH3 Domain of Yeast Abp1p.
E. J. Stollar, B. Garcia, P. A. Chong, A. Rath, H. Lin, J. D. Forman-Kay, and A. R. Davidson (2009)
J. Biol. Chem. 284, 26918-26927
   Abstract »    Full Text »    PDF »
She3p Possesses a Novel Activity Required for ASH1 mRNA Localization in Saccharomyces cerevisiae.
S. M. Landers, M. R. Gallas, J. Little, and R. M. Long (2009)
Eukaryot. Cell 8, 1072-1083
   Abstract »    Full Text »    PDF »
Shifted Transversal Design smart-pooling for high coverage interactome mapping.
X. Xin, J.-F. Rual, T. Hirozane-Kishikawa, D. E. Hill, M. Vidal, C. Boone, and N. Thierry-Mieg (2009)
Genome Res. 19, 1262-1269
   Abstract »    Full Text »    PDF »
Domain Interaction Footprint: a multi-classification approach to predict domain-peptide interactions.
C. Schillinger, P. Boisguerin, and G. Krause (2009)
Bioinformatics 25, 1632-1639
   Abstract »    Full Text »    PDF »
Role of Inn1 and its interactions with Hof1 and Cyk3 in promoting cleavage furrow and septum formation in S. cerevisiae.
R. Nishihama, J. H. Schreiter, M. Onishi, E. A. Vallen, J. Hanna, K. Moravcevic, M. F. Lippincott, H. Han, M. A. Lemmon, J. R. Pringle, et al. (2009)
J. Cell Biol. 185, 995-1012
   Abstract »    Full Text »    PDF »
Regulators of yeast endocytosis identified by systematic quantitative analysis.
H. E. Burston, L. Maldonado-Baez, M. Davey, B. Montpetit, C. Schluter, B. Wendland, and E. Conibear (2009)
J. Cell Biol. 185, 1097-1110
   Abstract »    Full Text »    PDF »
Charting the molecular network of the drug target Bcr-Abl.
M. Brehme, O. Hantschel, J. Colinge, I. Kaupe, M. Planyavsky, T. Kocher, K. Mechtler, K. L. Bennett, and G. Superti-Furga (2009)
PNAS 106, 7414-7419
   Abstract »    Full Text »    PDF »
Characterization of Domain-Peptide Interaction Interface: A Generic Structure-based Model to Decipher the Binding Specificity of SH3 Domains.
T. Hou, Z. Xu, W. Zhang, W. A. McLaughlin, D. A. Case, Y. Xu, and W. Wang (2009)
Mol. Cell. Proteomics 8, 639-649
   Abstract »    Full Text »    PDF »
The WASP Homologue Las17 Activates the Novel Actin-regulatory Activity of Ysc84 to Promote Endocytosis in Yeast.
A. S. Robertson, E. G. Allwood, A. P.C. Smith, F. C. Gardiner, R. Costa, S. J. Winder, and K. R. Ayscough (2009)
Mol. Biol. Cell 20, 1618-1628
   Abstract »    Full Text »    PDF »
The SH3 domains of two PCH family members cooperate in assembly of the Schizosaccharomyces pombe contractile ring.
R. H. Roberts-Galbraith, J.-S. Chen, J. Wang, and K. L. Gould (2009)
J. Cell Biol. 184, 113-127
   Abstract »    Full Text »    PDF »
DOA1/UFD3 Plays a Role in Sorting Ubiquitinated Membrane Proteins into Multivesicular Bodies.
J. Ren, N. Pashkova, S. Winistorfer, and R. C. Piper (2008)
J. Biol. Chem. 283, 21599-21611
   Abstract »    Full Text »    PDF »
Analysis of Unregulated Formin Activity Reveals How Yeast Can Balance F-Actin Assembly between Different Microfilament-based Organizations.
L. Gao and A. Bretscher (2008)
Mol. Biol. Cell 19, 1474-1484
   Abstract »    Full Text »    PDF »
Current progress in network research: toward reference networks for key model organisms.
B. S. Srinivasan, N. H. Shah, J. A. Flannick, E. Abeliuk, A. F. Novak, and S. Batzoglou (2007)
Brief Bioinform 8, 318-332
   Abstract »    Full Text »    PDF »
Interaction of the Endocytic Scaffold Protein Pan1 with the Type I Myosins Contributes to the Late Stages of Endocytosis.
S. L. Barker, L. Lee, B. D. Pierce, L. Maldonado-Baez, D. G. Drubin, and B. Wendland (2007)
Mol. Biol. Cell 18, 2893-2903
   Abstract »    Full Text »    PDF »
SH3-Hunter: discovery of SH3 domain interaction sites in proteins.
E. Ferraro, D. Peluso, A. Via, G. Ausiello, and M. Helmer-Citterich (2007)
Nucleic Acids Res. 35, W451-W454
   Abstract »    Full Text »    PDF »
Direct Binding to Rsp5p Regulates Ubiquitination-independent Vacuolar Transport of Sna3p.
H. Watson and J. S. Bonifacino (2007)
Mol. Biol. Cell 18, 1781-1789
   Abstract »    Full Text »    PDF »
Predicting protein-protein interactions based only on sequences information.
J. Shen, J. Zhang, X. Luo, W. Zhu, K. Yu, K. Chen, Y. Li, and H. Jiang (2007)
PNAS 104, 4337-4341
   Abstract »    Full Text »    PDF »
DOMINO: a database of domain-peptide interactions.
A. Ceol, A. Chatr-aryamontri, E. Santonico, R. Sacco, L. Castagnoli, and G. Cesareni (2007)
Nucleic Acids Res. 35, D557-D560
   Abstract »    Full Text »    PDF »
Hse1, a Component of the Yeast Hrs-STAM Ubiquitin-sorting Complex, Associates with Ubiquitin Peptidases and a Ligase to Control Sorting Efficiency into Multivesicular Bodies.
J. Ren, Y. Kee, J. M. Huibregtse, and R. C. Piper (2007)
Mol. Biol. Cell 18, 324-335
   Abstract »    Full Text »    PDF »
Fluorescent T7 display phages obtained by translational frameshift.
E. J. Slootweg, H. J.H.G. Keller, M. A. Hink, J. W. Borst, J. Bakker, and A. Schots (2006)
Nucleic Acids Res. 34, e137
   Abstract »    Full Text »    PDF »
A novel structure-based encoding for machine-learning applied to the inference of SH3 domain specificity.
E. Ferraro, A. Via, G. Ausiello, and M. Helmer-Citterich (2006)
Bioinformatics 22, 2333-2339
   Abstract »    Full Text »    PDF »
The Yeast Actin Cytoskeleton: from Cellular Function to Biochemical Mechanism.
J. B. Moseley and B. L. Goode (2006)
Microbiol. Mol. Biol. Rev. 70, 605-645
   Abstract »    Full Text »    PDF »
Transcript Profiles of Candida albicans Cortical Actin Patch Mutants Reflect Their Cellular Defects: Contribution of the Hog1p and Mkc1p Signaling Pathways.
U. Oberholzer, A. Nantel, J. Berman, and M. Whiteway (2006)
Eukaryot. Cell 5, 1252-1265
   Abstract »    Full Text »    PDF »
Rules for Modeling Signal-Transduction Systems.
W. S. Hlavacek, J. R. Faeder, M. L. Blinov, R. G. Posner, M. Hucka, and W. Fontana (2006)
Sci. STKE 2006, re6
   Abstract »    Full Text »    PDF »
An Integrated Machine Learning System to Computationally Screen Protein Databases for Protein Binding Peptide Ligands.
L. Zhang, C. Shao, D. Zheng, and Y. Gao (2006)
Mol. Cell. Proteomics 5, 1224-1232
   Abstract »    Full Text »    PDF »
Plasma membrane polarization during mating in yeast cells.
T. J. Proszynski, R. Klemm, M. Bagnat, K. Gaus, and K. Simons (2006)
J. Cell Biol. 173, 861-866
   Abstract »    Full Text »    PDF »
Discovering motif pairs at interaction sites from protein sequences on a proteome-wide scale.
H. Li, J. Li, and L. Wong (2006)
Bioinformatics 22, 989-996
   Abstract »    Full Text »    PDF »
Inference of gene regulatory networks and compound mode of action from time course gene expression profiles.
M. Bansal, G. D. Gatta, and D. di Bernardo (2006)
Bioinformatics 22, 815-822
   Abstract »    Full Text »    PDF »
The BAR Domain Proteins: Molding Membranes in Fission, Fusion, and Phagy.
G. Ren, P. Vajjhala, J. S. Lee, B. Winsor, and A. L. Munn (2006)
Microbiol. Mol. Biol. Rev. 70, 37-120
   Abstract »    Full Text »    PDF »
A regularized discriminative model for the prediction of protein-peptide interactions.
W. P. Lehrach, D. Husmeier, and C. K. I. Williams (2006)
Bioinformatics 22, 532-540
   Abstract »    Full Text »    PDF »
A unique set of SH3-SH3 interactions controls IB1 homodimerization.
O. Kristensen, S. Guenat, I. Dar, N. Allaman-Pillet, A. Abderrahmani, M. Ferdaoussi, R. Roduit, F. Maurer, J. S. Beckmann, J. S. Kastrup, et al. (2006)
EMBO J. 25, 785-797
   Abstract »    Full Text »    PDF »
Identification of preferred protein interactions by phage-display of the human Src homology-3 proteome.
S. Karkkainen, M. Hiipakka, J.-H. Wang, I. Kleino, M. Vaha-Jaakkola, G. H. Renkema, M. Liss, R. Wagner, and K. Saksela (2006)
EMBO Rep. 7, 186-191
   Abstract »    Full Text »    PDF »
Identification of VCP/p97, Carboxyl Terminus of Hsp70-interacting Protein (CHIP), and Amphiphysin II Interaction Partners Using Membrane-based Human Proteome Arrays.
G. Grelle, S. Kostka, A. Otto, B. Kersten, K. F. Genser, E.-C. Muller, S. Walter, A. Boddrich, U. Stelzl, C. Hanig, et al. (2006)
Mol. Cell. Proteomics 5, 234-244
   Abstract »    Full Text »    PDF »
Protein Function, Connectivity, and Duplicability in Yeast.
A. Prachumwat and W.-H. Li (2006)
Mol. Biol. Evol. 23, 30-39
   Abstract »    Full Text »    PDF »
Novel Src Homology 3 Domain-binding Motifs Identified from Proteomic Screen of a Pro-rich Region.
C. Y. H. Jia, J. Nie, C. Wu, C. Li, and S. S.-C. Li (2005)
Mol. Cell. Proteomics 4, 1155-1166
   Abstract »    Full Text »    PDF »
Structural and Functional Dissection of the Abp1 ADFH Actin-binding Domain Reveals Versatile In Vivo Adapter Functions.
O. Quintero-Monzon, A. A. Rodal, B. Strokopytov, S. C. Almo, and B. L. Goode (2005)
Mol. Biol. Cell 16, 3128-3139
   Abstract »    Full Text »    PDF »
Interaction of the Saccharomyces cerevisiae Cortical Actin Patch Protein Rvs167p With Proteins Involved in ER to Golgi Vesicle Trafficking.
H. Friesen, K. Colwill, K. Robertson, O. Schub, and B. Andrews (2005)
Genetics 170, 555-568
   Abstract »    Full Text »    PDF »
Interaction with the SH3 Domain Protein Bem1 Regulates Signaling by the Saccharomyces cerevisiae p21-Activated Kinase Ste20.
M. J. Winters and P. M. Pryciak (2005)
Mol. Cell. Biol. 25, 2177-2190
   Abstract »    Full Text »    PDF »
Rescue and In Situ Selection and Evaluation (RISE): A Method for High-Throughput Panning of Phage Display Libraries.
T. Vanhercke, C. Ampe, L. Tirry, and P. Denolf (2005)
J Biomol Screen 10, 108-117
   Abstract »    PDF »
Predicting protein-protein interactions using signature products.
S. Martin, D. Roe, and J.-L. Faulon (2005)
Bioinformatics 21, 218-226
   Abstract »    Full Text »    PDF »
MAP kinases and the adaptive response to hypertonicity: functional preservation from yeast to mammals.
D. Sheikh-Hamad and M. C. Gustin (2004)
Am J Physiol Renal Physiol 287, F1102-F1110
   Abstract »    Full Text »    PDF »
A Probabilistic Functional Network of Yeast Genes.
I. Lee, S. V. Date, A. T. Adai, and E. M. Marcotte (2004)
Science 306, 1555-1558
   Abstract »    Full Text »    PDF »
Recombinatorial Cloning Using Heterologous Lox Sites.
R. W. Siegel, N. Velappan, P. Pavlik, L. Chasteen, and A. Bradbury (2004)
Genome Res. 14, 1119-1129
   Abstract »    Full Text »    PDF »
Role of the cytoskeleton in signaling networks.
G. Forgacs, S. H. Yook, P. A. Janmey, H. Jeong, and C. G. Burd (2004)
J. Cell Sci. 117, 2769-2775
   Abstract »    Full Text »    PDF »
Selective inhibition of calcineurin-NFAT signaling by blocking protein-protein interaction with small organic molecules.
M. H. A. Roehrl, S. Kang, J. Aramburu, G. Wagner, A. Rao, and P. G. Hogan (2004)
PNAS 101, 7554-7559
   Abstract »    Full Text »    PDF »
Whole-genome annotation by using evidence integration in functional-linkage networks.
U. Karaoz, T. M. Murali, S. Letovsky, Y. Zheng, C. Ding, C. R. Cantor, and S. Kasif (2004)
PNAS 101, 2888-2893
   Abstract »    Full Text »    PDF »
Computational Alanine Scanning of Protein-Protein Interfaces.
T. Kortemme, D. E. Kim, and D. Baker (2004)
Sci. STKE 2004, pl2
   Abstract »    Full Text »    PDF »
WASp is required for the correct temporal morphogenesis of rhabdomere microvilli.
A. C. Zelhof and R. W. Hardy (2004)
J. Cell Biol. 164, 417-426
   Abstract »    Full Text »    PDF »
Distribution of proline-rich (PxxP) motifs in distinct proteomes: functional and therapeutic implications for malaria and tuberculosis.
B. Ravi Chandra, R. Gowthaman, R. Raj Akhouri, D. Gupta, and A. Sharma (2004)
Protein Eng. Des. Sel. 17, 175-182
   Abstract »    Full Text »    PDF »
Nbp2 targets the Ptc1-type 2C Ser/Thr phosphatase to the HOG MAPK pathway.
J. Mapes and I. M. Ota (2004)
EMBO J. 23, 302-311
   Abstract »    Full Text »    PDF »
Fus1p Interacts With Components of the Hog1p Mitogen-Activated Protein Kinase and Cdc42p Morphogenesis Signaling Pathways to Control Cell Fusion During Yeast Mating.
B. Nelson, A. B. Parsons, M. Evangelista, K. Schaefer, K. Kennedy, S. Ritchie, T. L. Petryshen, and C. Boone (2004)
Genetics 166, 67-77
   Abstract »    Full Text »    PDF »
Structural Insight into Modest Binding of a Non-PXXP Ligand to the Signal Transducing Adaptor Molecule-2 Src Homology 3 Domain.
T. Kaneko, T. Kumasaka, T. Ganbe, T. Sato, K. Miyazawa, N. Kitamura, and N. Tanaka (2003)
J. Biol. Chem. 278, 48162-48168
   Abstract »    Full Text »    PDF »
Disease genes and intracellular protein networks.
S. Bortoluzzi, C. Romualdi, A. Bisognin, and G. A. Danieli (2003)
Physiol Genomics 15, 223-227
   Abstract »    Full Text »    PDF »
Evolution of the yeast protein interaction network.
H. Qin, H. H. S. Lu, W. B. Wu, and W.-H. Li (2003)
PNAS 100, 12820-12824
   Abstract »    Full Text »    PDF »
The PCH family protein, Cdc15p, recruits two F-actin nucleation pathways to coordinate cytokinetic actin ring formation in Schizosaccharomyces pombe.
R. H. Carnahan and K. L. Gould (2003)
J. Cell Biol. 162, 851-862
   Abstract »    Full Text »    PDF »
DePIE: Designing Primers for Protein Interaction Experiments.
G. Lu, M. Hallett, S. Pollock, and D. Thomas (2003)
Nucleic Acids Res. 31, 3755-3757
   Abstract »    Full Text »    PDF »
Formins: signaling effectors for assembly and polarization of actin filaments.
M. Evangelista, S. Zigmond, and C. Boone (2003)
J. Cell Sci. 116, 2603-2611
   Abstract »    Full Text »    PDF »
Regulation of the Yeast Amphiphysin Homologue Rvs167p by Phosphorylation.
H. Friesen, K. Murphy, A. Breitkreutz, M. Tyers, and B. Andrews (2003)
Mol. Biol. Cell 14, 3027-3040
   Abstract »    Full Text »    PDF »
The Structure and Function of Proline Recognition Domains.
A. Zarrinpar, R. P. Bhattacharyya, and W. A. Lim (2003)
Sci. STKE 2003, re8
   Abstract »    Full Text »    PDF »
Assembly of Cell Regulatory Systems Through Protein Interaction Domains.
T. Pawson and P. Nash (2003)
Science 300, 445-452
   Abstract »    Full Text »    PDF »
Helicobacter pylori: After the Genomes, Back to Biology.
A. Covacci and R. Rappuoli (2003)
J. Exp. Med. 197, 807-811
   Full Text »    PDF »
Super models.
M. M. Barr (2003)
Physiol Genomics 13, 15-24
   Abstract »    Full Text »    PDF »
BIND: the Biomolecular Interaction Network Database.
G. D. Bader, D. Betel, and C. W. V. Hogue (2003)
Nucleic Acids Res. 31, 248-250
   Abstract »    Full Text »    PDF »
Saccharomyces cerevisiae Bzz1p Is Implicated with Type I Myosins in Actin Patch Polarization and Is Able To Recruit Actin-Polymerizing Machinery In Vitro.
A. Soulard, T. Lechler, V. Spiridonov, A. Shevchenko, A. Shevchenko, R. Li, and B. Winsor (2002)
Mol. Cell. Biol. 22, 7889-7906
   Abstract »    Full Text »    PDF »
Significance of GTP Hydrolysis in Ypt1p-regulated Endoplasmic Reticulum to Golgi Transport Revealed by the Analysis of Two Novel Ypt1-GAPs.
A. De Antoni, J. Schmitzova, H.-H. Trepte, D. Gallwitz, and S. Albert (2002)
J. Biol. Chem. 277, 41023-41031
   Abstract »    Full Text »    PDF »
In Vitro Evolution of Recognition Specificity Mediated by SH3 Domains Reveals Target Recognition Rules.
S. Panni, L. Dente, and G. Cesareni (2002)
J. Biol. Chem. 277, 21666-21674
   Abstract »    Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882