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Science 295 (5556): 868-872

Copyright © 2002 by the American Association for the Advancement of Science

Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

Carlos Lois,* Elizabeth J. Hong,* Shirley Pease, Eric J. Brown, David Baltimoredagger

Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.

Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: baltimo{at}caltech.edu



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AAV and Compacted DNA Nanoparticles for the Treatment of Retinal Disorders: Challenges and Future Prospects.
Z. Han, S. M. Conley, and M. I. Naash (2011)
Invest. Ophthalmol. Vis. Sci. 52, 3051-3059
   Abstract »    Full Text »    PDF »
PSD-95 Is Required to Sustain the Molecular Organization of the Postsynaptic Density.
X. Chen, C. D. Nelson, X. Li, C. A. Winters, R. Azzam, A. A. Sousa, R. D. Leapman, H. Gainer, M. Sheng, and T. S. Reese (2011)
J. Neurosci. 31, 6329-6338
   Abstract »    Full Text »    PDF »
EphA4 preserves postnatal and adult neural stem cells in an undifferentiated state in vivo.
K. Khodosevich, Y. Watanabe, and H. Monyer (2011)
J. Cell Sci. 124, 1268-1279
   Abstract »    Full Text »    PDF »
A Requirement for Nuclear Factor-{kappa}B in Developmental and Plasticity-Associated Synaptogenesis.
M. C. H. Boersma, E. C. Dresselhaus, L. M. De Biase, A. B. Mihalas, D. E. Bergles, and M. K. Meffert (2011)
J. Neurosci. 31, 5414-5425
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Generation of Transgenic Mice on an NOD/SCID Background Using the Conventional Microinjection Technique.
K. Kumagai, N. Kubota, T. I. Saito, T. Sasako, R. Takizawa, K. Sudo, M. Kurokawa, and T. Kadowaki (2011)
Biol Reprod 84, 682-688
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Signal Transduction of Pregnenolone Sulfate in Insulinoma Cells: ACTIVATION OF EGR-1 EXPRESSION INVOLVING TRPM3, VOLTAGE-GATED CALCIUM CHANNELS, ERK, AND TERNARY COMPLEX FACTORS.
S. I. Mayer, I. Muller, S. Mannebach, T. Endo, and G. Thiel (2011)
J. Biol. Chem. 286, 10084-10096
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Pten Knockdown In Vivo Increases Excitatory Drive onto Dentate Granule Cells.
B. W. Luikart, E. Schnell, E. K. Washburn, A. L. Bensen, K. R. Tovar, and G. L. Westbrook (2011)
J. Neurosci. 31, 4345-4354
   Abstract »    Full Text »    PDF »

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