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Science 295 (5556): 868-872

Copyright © 2002 by the American Association for the Advancement of Science

Germline Transmission and Tissue-Specific Expression of Transgenes Delivered by Lentiviral Vectors

Carlos Lois,* Elizabeth J. Hong,* Shirley Pease, Eric J. Brown, David Baltimoredagger

Single-cell mouse embryos were infected in vitro with recombinant lentiviral vectors to generate transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter. Eighty percent of founder mice carried at least one copy of the transgene, and 90% of these expressed GFP at high levels. Progeny inherited the transgene(s) and displayed green fluorescence. Mice generated using lentiviral vectors with muscle-specific and T lymphocyte-specific promoters expressed high levels of GFP only in the appropriate cell types. We have also generated transgenic rats that express GFP at high levels, suggesting that this technique can be used to produce other transgenic animal species.

Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
*   These authors contributed equally to this work.

dagger    To whom correspondence should be addressed. E-mail: baltimo{at}

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J. Am. Soc. Nephrol.
   Abstract »
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R. Roset, A. Inagaki, M. Hohl, F. Brenet, J. Lafrance-Vanasse, J. Lange, J. M. Scandura, J. A. Tainer, S. Keeney, and J. H. J. Petrini (2014)
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   Abstract »    Full Text »    PDF »
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A. W. Halff, D. Gomez-Varela, D. John, and D. K. Berg (2014)
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   Abstract »    Full Text »    PDF »
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M. Liu, L. D. Lewis, R. Shi, E. N. Brown, and W. Xu (2014)
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   Abstract »    Full Text »    PDF »
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A. Chandrashekran, R. Sarkar, A. Thrasher, S. E. Fraser, N. Dibb, C. Casimir, R. Winston, and C. Readhead (2014)
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   Abstract »    Full Text »    PDF »
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H. Y. Park, H. Lim, Y. J. Yoon, A. Follenzi, C. Nwokafor, M. Lopez-Jones, X. Meng, and R. H. Singer (2014)
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   Abstract »    Full Text »    PDF »
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J. Neurosci. 34, 855-868
   Abstract »    Full Text »    PDF »
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G. L. Kelly, S. Grabow, S. P. Glaser, L. Fitzsimmons, B. J. Aubrey, T. Okamoto, L. J. Valente, M. Robati, L. Tai, W. D. Fairlie, et al. (2014)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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S. Loebrich, B. Djukic, Z. J. Tong, J. R. Cottrell, G. G. Turrigiano, and E. Nedivi (2013)
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   Abstract »    Full Text »    PDF »
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A. Datta, R. Alexander, M. G. Sulikowski, A. G. Nicholson, T. M. Maher, C. J. Scotton, and R. C. Chambers (2013)
J. Immunol. 191, 4867-4879
   Abstract »    Full Text »    PDF »
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L. Zeng, Y. Wan, D. Li, J. Wu, M. Shao, J. Chen, L. Hui, H. Ji, and X. Zhu (2013)
J. Biol. Chem. 288, 30087-30093
   Abstract »    Full Text »    PDF »
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M. Arancillo, S.-W. Min, S. Gerber, A. Munster-Wandowski, Y.-J. Wu, M. Herman, T. Trimbuch, J.-C. Rah, G. Ahnert-Hilger, D. Riedel, et al. (2013)
J. Neurosci. 33, 16698-16714
   Abstract »    Full Text »    PDF »
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E. G. Govorunova, O. A. Sineshchekov, H. Li, R. Janz, and J. L. Spudich (2013)
J. Biol. Chem. 288, 29911-29922
   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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Y. Zhang, G. Jiang, M. Sauler, and P. J. Lee (2013)
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   Abstract »    Full Text »    PDF »
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J. M. Kruger, P. D. Favaro, M. Liu, A. Kitlinska, X. Huang, M. Raabe, D. S. Akad, Y. Liu, H. Urlaub, Y. Dong, et al. (2013)
J. Neurosci. 33, 15504-15517
   Abstract »    Full Text »    PDF »
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L. Iwai, Y. Ohashi, D. van der List, W. M. Usrey, Y. Miyashita, and H. Kawasaki (2013)
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   Abstract »    Full Text »    PDF »
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S. H. Lee, M. K. Gupta, Y. T. Ho, T. Kim, and H. T. Lee (2013)
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   Abstract »    Full Text »    PDF »
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K. Ivankova, R. Turecek, T. Fritzius, R. Seddik, L. Prezeau, L. Comps-Agrar, J.-P. Pin, B. Fakler, V. Besseyrias, M. Gassmann, et al. (2013)
J. Biol. Chem. 288, 24848-24856
   Abstract »    Full Text »    PDF »
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E. L. Dimitrov, J. Kuo, K. Kohno, and T. B. Usdin (2013)
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   Abstract »    Full Text »    PDF »
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B. D. Hopkins, B. Fine, N. Steinbach, M. Dendy, Z. Rapp, J. Shaw, K. Pappas, J. S. Yu, C. Hodakoski, S. Mense, et al. (2013)
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   Abstract »    Full Text »    PDF »
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I. Gonzalez-Vasconcellos, N. Anastasov, B. Sanli-Bonazzi, O. Klymenko, M. J. Atkinson, and M. Rosemann (2013)
Cancer Res. 73, 4247-4255
   Abstract »    Full Text »    PDF »
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S. M. Foss, H. Li, M. S. Santos, R. H. Edwards, and S. M. Voglmaier (2013)
J. Neurosci. 33, 10647-10660
   Abstract »    Full Text »    PDF »
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Q. Guo, J. Zhang, J. Li, L. Zou, J. Zhang, Z. Xie, X. Fu, S. Jiang, G. Chen, Q. Jia, et al. (2013)
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   Abstract »    Full Text »    PDF »
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G. Andrey, T. Montavon, B. Mascrez, F. Gonzalez, D. Noordermeer, M. Leleu, D. Trono, F. Spitz, and D. Duboule (2013)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. Exp. Med. 210, 789-803
   Abstract »    Full Text »    PDF »
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J. V. Perederiy, B. W. Luikart, E. K. Washburn, E. Schnell, and G. L. Westbrook (2013)
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   Abstract »    Full Text »    PDF »
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J. Neurosci. 33, 5040-5052
   Abstract »    Full Text »    PDF »
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K. Katter, A. M. Geurts, O. Hoffmann, L. Mates, V. Landa, L. Hiripi, C. Moreno, J. Lazar, S. Bashir, V. Zidek, et al. (2013)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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Y. S. Kim, E. Kang, Y. Makino, S. Park, J. H. Shin, H. Song, P. Launay, and D. J. Linden (2013)
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   Abstract »    Full Text »    PDF »
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K. Palomares, F. Vigant, B. Van Handel, O. Pernet, K. Chikere, P. Hong, S. P. Sherman, M. Patterson, D. S. An, W. E. Lowry, et al. (2013)
J. Virol. 87, 2094-2108
   Abstract »    Full Text »    PDF »
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H. Kawasaki, T. Toda, and K. Tanno (2013)
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   Abstract »    Full Text »    PDF »
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K. Furushima, C.-W. Jang, D. W. Chen, N. Xiao, P. A. Overbeek, and R. R. Behringer (2012)
Genetics 192, 1235-1248
   Abstract »    Full Text »    PDF »
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E. M. Hur, S. N. Patel, S. Shimizu, D. S. Rao, P. N. P. Gnanapragasam, D. S. An, L. Yang, and D. Baltimore (2012)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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J. S. Dickey, B. J. Baird, C. E. Redon, V. Avdoshina, G. Palchik, J. Wu, A. Kondratyev, W. M. Bonner, and O. A. Martin (2012)
Nucleic Acids Res. 40, 10274-10286
   Abstract »    Full Text »    PDF »
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B. T. Selvaraj, N. Frank, F. L. P. Bender, E. Asan, and M. Sendtner (2012)
J. Cell Biol. 199, 437-451
   Abstract »    Full Text »    PDF »
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T. Stoyanova, A. S. Goldstein, H. Cai, J. M. Drake, J. Huang, and O. N. Witte (2012)
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   Abstract »    Full Text »    PDF »
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J. Kuhse, H. Kalbouneh, A. Schlicksupp, S. Mukusch, R. Nawrotzki, and J. Kirsch (2012)
J. Biol. Chem. 287, 30952-30966
   Abstract »    Full Text »    PDF »
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W. Kelsch, Z. Li, M. Eliava, C. Goengrich, and H. Monyer (2012)
J. Neurosci. 32, 12603-12611
   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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W. Yi, P. M. Clark, D. E. Mason, M. C. Keenan, C. Hill, W. A. Goddard III, E. C. Peters, E. M. Driggers, and L. C. Hsieh-Wilson (2012)
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   Abstract »    Full Text »    PDF »
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M. C. Weston, H. Chen, and J. W. Swann (2012)
J. Neurosci. 32, 11441-11452
   Abstract »    Full Text »    PDF »
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P. W. Bryant, Q. Zheng, and K. M. Pumiglia (2012)
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   Abstract »    Full Text »    PDF »
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C. Maas, V. I. Torres, W. D. Altrock, S. Leal-Ortiz, D. Wagh, R. T. Terry-Lorenzo, A. Fejtova, E. D. Gundelfinger, N. E. Ziv, and C. C. Garner (2012)
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   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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M. Akerblom, R. Sachdeva, I. Barde, S. Verp, B. Gentner, D. Trono, and J. Jakobsson (2012)
J. Neurosci. 32, 8879-8889
   Abstract »    Full Text »    PDF »
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M. Wu, L. Shi, A. Cimic, L. Romero, G. Sui, C. J. Lees, J. M. Cline, D. F. Seals, J. S. Sirintrapun, T. P. McCoy, et al. (2012)
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   Abstract »    Full Text »    PDF »
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Y. Kageyama, Z. Zhang, R. Roda, M. Fukaya, J. Wakabayashi, N. Wakabayashi, T. W. Kensler, P. H. Reddy, M. Iijima, and H. Sesaki (2012)
J. Cell Biol. 197, 535-551
   Abstract »    Full Text »    PDF »
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G. Xu, K. Liu, J. Anderson, K. Patrene, S. Lentzsch, G. D. Roodman, and H. Ouyang (2012)
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   Abstract »    Full Text »    PDF »
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F. Niere, J. R. Wilkerson, and K. M. Huber (2012)
J. Neurosci. 32, 5924-5936
   Abstract »    Full Text »    PDF »
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L. Jia, R. Wang, and D. D. Tang (2012)
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   Abstract »    Full Text »    PDF »
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M. Sumegi, Y. Fukazawa, K. Matsui, A. Lorincz, M. D. Eyre, Z. Nusser, and R. Shigemoto (2012)
J. Physiol. 590, 1517-1534
   Abstract »    Full Text »    PDF »
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M. C. Mulvey, K. A. Sacksteder, L. Einck, and C. A. Nacy (2012)
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   Abstract »    Full Text »    PDF »
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L. Rodriguez-Menocal, M. Salgado, D. Ford, and E. Van Badiavas (2012)
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   Abstract »    Full Text »    PDF »
CSP{alpha} knockout causes neurodegeneration by impairing SNAP-25 function.
M. Sharma, J. Burre, P. Bronk, Y. Zhang, W. Xu, and T. C. Sudhof (2012)
EMBO J. 31, 829-841
   Abstract »    Full Text »    PDF »
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B. Damiri, E. Holle, X. Yu, and W. S. Baldwin (2012)
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   Abstract »    Full Text »    PDF »
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A. H. Klopp, Y. Zhang, T. Solley, F. Amaya-Manzanares, F. Marini, M. Andreeff, B. Debeb, W. Woodward, R. Schmandt, R. Broaddus, et al. (2012)
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   Abstract »    Full Text »    PDF »
Wandering Neuronal Migration in the Postnatal Vertebrate Forebrain.
B. B. Scott, T. Gardner, N. Ji, M. S. Fee, and C. Lois (2012)
J. Neurosci. 32, 1436-1446
   Abstract »    Full Text »    PDF »
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C. C. Chang, L. Krishnan, S. S. Nunes, K. H. Church, L. T. Edgar, E. D. Boland, J. A. Weiss, S. K. Williams, and J. B. Hoying (2012)
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   Abstract »    Full Text »    PDF »
pTransgenesis: a cross-species, modular transgenesis resource.
N. R. Love, R. Thuret, Y. Chen, S. Ishibashi, N. Sabherwal, R. Paredes, J. Alves-Silva, K. Dorey, A. M. Noble, M. J. Guille, et al. (2011)
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   Abstract »    Full Text »    PDF »
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B. P. Hermann, M. Sukhwani, J. Salati, Y. Sheng, T. Chu, and K. E. Orwig (2011)
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   Abstract »    Full Text »    PDF »
Use Dependence of Presynaptic Tenacity.
A. Fisher-Lavie, A. Zeidan, M. Stern, C. C. Garner, and N. E. Ziv (2011)
J. Neurosci. 31, 16770-16780
   Abstract »    Full Text »    PDF »
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D. R. Cook, P. A. Solski, S. J. Bultman, G. Kauselmann, M. Schoor, R. Kuehn, L. S. Friedman, D. O. Cowley, T. Van Dyke, J. J. Yeh, et al. (2011)
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   Abstract »    Full Text »    PDF »
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D. Li, C. G. Specht, C. L. Waites, C. Butler-Munro, S. Leal-Ortiz, J. W. Foote, D. Genoux, C. C. Garner, and J. M. Montgomery (2011)
J. Physiol. 589, 4491-4510
   Abstract »    Full Text »    PDF »
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S. L. Edelmann, P. J. Nelson, and T. Brocker (2011)
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   Abstract »    Full Text »    PDF »
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Z. Xie, L. Wroblewska, L. Prochazka, R. Weiss, and Y. Benenson (2011)
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   Abstract »    Full Text »    PDF »
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S. Sripathy, M. Lee, and V. Vasioukhin (2011)
Mol. Cell. Biol. 31, 2920-2933
   Abstract »    Full Text »    PDF »
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K. Welshhans and G. J. Bassell (2011)
J. Neurosci. 31, 9800-9813
   Abstract »    Full Text »    PDF »
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A. L. Suarez, R. Kong, T. George, L. He, Z. Yue, and L. F. van Dyk (2011)
J. Virol. 85, 6293-6308
   Abstract »    Full Text »    PDF »
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M. P. Boldin, K. D. Taganov, D. S. Rao, L. Yang, J. L. Zhao, M. Kalwani, Y. Garcia-Flores, M. Luong, A. Devrekanli, J. Xu, et al. (2011)
J. Exp. Med. 208, 1189-1201
   Abstract »    Full Text »    PDF »
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   Abstract »    Full Text »    PDF »
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A. Kajaste-Rudnitski, S. S. Marelli, C. Pultrone, T. Pertel, P. D. Uchil, N. Mechti, W. Mothes, G. Poli, J. Luban, and E. Vicenzi (2011)
J. Virol. 85, 5183-5196
   Abstract »    Full Text »    PDF »
Peripheral T Cells Re-Enter the Thymus and Interfere with Central Tolerance Induction.
S. L. Edelmann, P. Marconi, and T. Brocker (2011)
J. Immunol. 186, 5612-5619
   Abstract »    Full Text »    PDF »
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T.-F. Chou and R. J. Deshaies (2011)
J. Biol. Chem. 286, 16546-16554
   Abstract »    Full Text »    PDF »
Site-specific integrase-mediated transgenesis in mice via pronuclear injection.
B. Tasic, S. Hippenmeyer, C. Wang, M. Gamboa, H. Zong, Y. Chen-Tsai, and L. Luo (2011)
PNAS 108, 7902-7907
   Abstract »    Full Text »    PDF »
AAV and Compacted DNA Nanoparticles for the Treatment of Retinal Disorders: Challenges and Future Prospects.
Z. Han, S. M. Conley, and M. I. Naash (2011)
Invest. Ophthalmol. Vis. Sci. 52, 3051-3059
   Abstract »    Full Text »    PDF »
PSD-95 Is Required to Sustain the Molecular Organization of the Postsynaptic Density.
X. Chen, C. D. Nelson, X. Li, C. A. Winters, R. Azzam, A. A. Sousa, R. D. Leapman, H. Gainer, M. Sheng, and T. S. Reese (2011)
J. Neurosci. 31, 6329-6338
   Abstract »    Full Text »    PDF »
EphA4 preserves postnatal and adult neural stem cells in an undifferentiated state in vivo.
K. Khodosevich, Y. Watanabe, and H. Monyer (2011)
J. Cell Sci. 124, 1268-1279
   Abstract »    Full Text »    PDF »
A Requirement for Nuclear Factor-{kappa}B in Developmental and Plasticity-Associated Synaptogenesis.
M. C. H. Boersma, E. C. Dresselhaus, L. M. De Biase, A. B. Mihalas, D. E. Bergles, and M. K. Meffert (2011)
J. Neurosci. 31, 5414-5425
   Abstract »    Full Text »    PDF »
Generation of Transgenic Mice on an NOD/SCID Background Using the Conventional Microinjection Technique.
K. Kumagai, N. Kubota, T. I. Saito, T. Sasako, R. Takizawa, K. Sudo, M. Kurokawa, and T. Kadowaki (2011)
Biol Reprod 84, 682-688
   Abstract »    Full Text »    PDF »
S. I. Mayer, I. Muller, S. Mannebach, T. Endo, and G. Thiel (2011)
J. Biol. Chem. 286, 10084-10096
   Abstract »    Full Text »    PDF »
Pten Knockdown In Vivo Increases Excitatory Drive onto Dentate Granule Cells.
B. W. Luikart, E. Schnell, E. K. Washburn, A. L. Bensen, K. R. Tovar, and G. L. Westbrook (2011)
J. Neurosci. 31, 4345-4354
   Abstract »    Full Text »    PDF »

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