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Cytoskeletal Regulation by the Nedd8 Ubiquitin-Like Protein Modification Pathway
Thimo Kurz,1Lionel Pintard,2John H. Willis,1Danielle R. Hamill,1*Pierre Gönczy,2Matthias Peter,2Bruce Bowerman1
The Nedd8 ubiquitin-like protein modification pathway regulates
cell-cycle progression. Our analysis of Nedd8 requirementsduring
Caenorhabditis elegans embryogenesis indicates that thecytoskeleton is another target. Nedd8 conjugation negatively regulatedcontractility of the microfilament-rich cell cortex during pronuclearmigration and again during cytokinesis. The Nedd8 pathway alsowas
required after meiosis to negatively regulate katanin, a
microtubule-severingcomplex, permitting the assembly of a large
mitotic spindle. Wepropose that Nedd8-modified cullin, as part of an
E3 ubiquitinligase complex, targets katanin for degradation during the
transitionfrom meiosis to mitosis.
1 Institute of Molecular Biology, University of
Oregon, Eugene, OR 97403, USA.
2 Swiss Institute for
Experimental Cancer Research (ISREC), CH-1066 Lausanne, Switzerland.
*
Present address: Department of Zoology, Ohio Wesleyan
University, Delaware, OH 43015, USA.
To whom correspondence should be addressed. E-mail:
bbowerman{at}molbio.uoregon.edu
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