Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Subscribe

Logo for

Science 295 (5561): 1910-1912

Copyright © 2002 by the American Association for the Advancement of Science

Parallel Single-Cell Monitoring of Receptor-Triggered Membrane Translocation of a Calcium-Sensing Protein Module

Mary N. Teruel, Tobias Meyer*

Time courses of translocation of fluorescently conjugated proteins to the plasma membrane were simultaneously measured in thousands of individual rat basophilic leukemia cells. We found that the C2 domain--a calcium-sensing, lipid-binding protein module that is an essential regulator of protein kinase C and numerous other proteins--targeted proteins to the plasma membrane transiently if calcium was released from internal stores, and persistently in response to entry of extracellular calcium across the plasma membrane. The C2 domain translocation time courses of stimulated cells clustered into only two primary modes. Hence, the reversible recruitment of families of signaling proteins from one cellular compartment to another is a rapid bifurcation mechanism for inducing discrete states of cellular signaling networks.

Department of Molecular Pharmacology, Stanford University Medical School, 269 Campus Drive, Stanford, CA 94305, USA.
*   To whom correspondence should be addressed. E-mail: tobiasmeyer{at}stanford.edu

THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Arabidopsis thaliana calcium-dependent lipid-binding protein (AtCLB): a novel repressor of abiotic stress response.
K. de Silva, B. Laska, C. Brown, H. W. Sederoff, and M. Khodakovskaya (2011)
J. Exp. Bot. 62, 2679-2689
   Abstract »    Full Text »    PDF »
Characterization of a canola C2 domain gene that interacts with PG, an effector of the necrotrophic fungus Sclerotinia sclerotiorum.
X. Wang, Q. Li, X. Niu, H. Chen, L. Xu, and C. Qi (2009)
J. Exp. Bot. 60, 2613-2620
   Abstract »    Full Text »    PDF »
High Content Cell Screening in a Microfluidic Device.
R. Cheong, C. J. Wang, and A. Levchenko (2009)
Mol. Cell. Proteomics 8, 433-442
   Abstract »    Full Text »    PDF »
Fluorescence resonance energy transfer imaging of PKC signalling in living cells using genetically encoded fluorescent probes.
J. Goedhart and T. W.J Gadella Jr (2009)
J R Soc Interface 6, S27-S34
   Abstract »    Full Text »    PDF »
Regulation of PLC{beta}1a membrane anchoring by its substrate phosphatidylinositol (4,5)-bisphosphate.
M. J. W. Adjobo-Hermans, J. Goedhart, and T. W. J. Gadella Jr (2008)
J. Cell Sci. 121, 3770-3777
   Abstract »    Full Text »    PDF »
New frontiers in single-cell analysis.
R. H Templer and O. Ces (2008)
J R Soc Interface 5, S111-S112
   Abstract »    Full Text »    PDF »
Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool.
P. M.P Lanigan, K. Chan, T. Ninkovic, R. H Templer, P.M.W French, A.J de Mello, K.R Willison, P.J Parker, M.A.A Neil, O. Ces, et al. (2008)
J R Soc Interface 5, S161-S168
   Abstract »    Full Text »    PDF »
Principles of Total Internal Reflection Microscopy (TIRFM).
D. Zenisek and D. Perrais (2007)
Cold Spring Harb Protoc 2007, pdb.top24
   Abstract »    Full Text »
Reversible intracellular translocation of KRas but not HRas in hippocampal neurons regulated by Ca2+/calmodulin.
M. Fivaz and T. Meyer (2005)
J. Cell Biol. 170, 429-441
   Abstract »    Full Text »    PDF »
Mechanisms of Arginine-Vasopressin-Induced Ca2+ Oscillations in {beta}-Cells (HIT-T15): A Role for Oscillating Protein Kinase C.
M. Schaefer, H. Mischak, S. Schnell, A. Griese, R. Iakubov, G. Riepenhausen, and C. Schofl (2004)
Endocrinology 145, 4635-4644
   Abstract »    Full Text »    PDF »
The Cytosolic Phospholipase A2 Catalytic Domain Modulates Association and Residence Time at Golgi Membranes.
J. H. Evans and C. C. Leslie (2004)
J. Biol. Chem. 279, 6005-6016
   Abstract »    Full Text »    PDF »
The Calcium Binding Loops of the Cytosolic Phospholipase A2 C2 Domain Specify Targeting to Golgi and ER in Live Cells.
J. H. Evans, S. H. Gerber, D. Murray, and C. C. Leslie (2004)
Mol. Biol. Cell 15, 371-383
   Abstract »    Full Text »    PDF »
Role of the Ca2+/Phosphatidylserine Binding Region of the C2 Domain in the Translocation of Protein Kinase Calpha to the Plasma Membrane.
S. R. Bolsover, J. C. Gomez-Fernandez, and S. Corbalan-Garcia (2003)
J. Biol. Chem. 278, 10282-10290
   Abstract »    Full Text »    PDF »
IGF-I-Induced Phosphorylation of Connexin 43 by PKC{gamma}: Regulation of Gap Junctions in Rabbit Lens Epithelial Cells.
D. Lin, D. L. Boyle, and D. J. Takemoto (2003)
Invest. Ophthalmol. Vis. Sci. 44, 1160-1168
   Abstract »    Full Text »    PDF »
Single Cell Imaging of PI3K Activity and Glucose Transporter Insertion Into the Plasma Membrane by Dual Color Evanescent Wave Microscopy.
A. Tengholm, M. N. Teruel, and T. Meyer (2003)
Sci. STKE 2003, pl4
   Abstract »    Full Text »    PDF »
Shedding Light on Cell Signaling: Interpretation of FRET Biosensors.
F. Gaits and K. Hahn (2003)
Sci. STKE 2003, pe3
   Abstract »    Full Text »    PDF »
Send Me a Message: The FASEB Conference on Signal Transduction Salt Lake City, UT June 29-July 3, 2002.
W. A. Wells (2002)
J. Cell Biol. 158, 994-995
   Full Text »    PDF »

To Advertise     Find Products

Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882