The Antibacterial Lectin RegIII Promotes the Spatial Segregation of Microbiota and Host in the Intestine
Kari M. Severson1,
Kelly A. Ruhn1,
Edward K. Wakeland1, and
Lora V. Hooper1,2,*
1 Department of Immunology, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
2 The Howard Hughes Medical Institute, The University of Texas Southwestern Medical Center at Dallas, Dallas, TX 75390, USA.
3 Department of Microbiology, Cornell University, Ithaca, NY 14853, USA.
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Fig. 1. MyD88 promotes physical separation of the microbiota and the small intestinal surface. (A) Visualization of microbiota localization relative to the small intestinal mucosal surface by FISH. Sections were hybridized to a probe that recognizes the 16S rRNA genes of all bacteria (green) and counterstained with 4',6-diamidino-2-phenylindole (DAPI) to visualize nuclei (blue). Scale bars, 50 μm. Arrows indicate the distance from the villus tip to the microbiota. Mice were cohoused littermates from intercrossed Myd88+/– mice. Sections are representative of >10 groups of littermates. (B) Quantification of fluorescence intensity extending from the villus tip into the lumen (N = 5 mice per genotype). (C) Mucosa-associated and luminal bacteria were quantified by Q-PCR determination of 16S rRNA gene copy number in the terminal ileum. N = 5 mice per genotype. Data are from three groups of littermates. *, P < 0.05; error bars, mean ±SEM; ns, not significant.|