SUMOylation Silences Heterodimeric TASK Potassium Channels Containing K2P1 Subunits in Cerebellar Granule Neurons

Leigh D. Plant, Leandro Zuniga, Dan Araki, Jeremy D. Marks, Steve A. N. Goldstein*

*To whom correspondence should be addressed. E-mail: goldstein{at}brandeis.edu

This PDF file includes:

• Fig. S1. KCNK1, KCNK3, and KCNK9 transcripts are present together in single cerebellar granule neurons.
• Fig. S2. Antibodies to K2P1, K2P3, and K3P9 are specific.
• Fig. S3. Antibody-mediated FRET demonstrates that interaction between K2P9 and SUMO1 requires K2P1.
• Fig. S4. FRET indicates that K2P2 does not interact with K2P1, K2P3, K2P9, or SUMO1.
• Fig. S5. Whole-cell recording of K2P1 expressed with K2P3 or K2P9 in CHO cells.
• Fig. S6. Silencing by SUMO1 and SUMO2 is independent of the order of K2P subunits in tandem constructs.
• Fig. S7. Studies of K2P1-Y231F subunits expressed in CHO cells or cerebellar granule neurons.
• Table S1. Biophysical parameters for cerebellar granule neurons.
• Table S2. Donor decay time constants indicate those K2P subunits that interact.
• Table S3. Donor decay time constants indicate that K2P1 is required for interaction of YFP-SUMO1 and CFP-K2P3 or CFP-K2P9 in CHO cells and cerebellar granule neurons.
• Movie S1 legend
  

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi format). Projection of fluorescently labeled KCNK transcripts in a single cerebellar granule neuron.

© 2012 American Association for the Advancement of Science