Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 18 December 2012
[DOI: 10.1126/scisignal.2003558]

Supplementary Materials for:

c-FLIP Maintains Tissue Homeostasis by Preventing Apoptosis and Programmed Necrosis

Xuehua Piao, Sachiko Komazawa-Sakon, Takashi Nishina, Masato Koike, Jiang-Hu Piao, Hanno Ehlken, Hidetake Kurihara, Mutsuko Hara, Nico Van Rooijen, Günther Schütz, Masaki Ohmuraya, Yasuo Uchiyama, Hideo Yagita, Ko Okumura, You-Wen He, Hiroyasu Nakano*

*To whom correspondence should be addressed. E-mail: hnakano{at}juntendo.ac.jp

This PDF file includes:

  • Fig. S1. Histological analysis of the small intestine and colon.
  • Fig. S2. c-FlipF/F;Villin-Cre;Tnfr1+/– mice do not develop colitis.
  • Fig. S3. ConA-induced hepatitis is exacerbated in c-FlipF/F;Alb-Cre mice.
  • Fig. S4. Degradation of RIPK1 protein correlates with selective induction of apoptosis in the liver.
  • Fig. S5. c-FLIP in hepatocytes, but not hematopoietic cells, plays a crucial role in the protection of hepatocytes from cell death.
  • Fig. S6. Administration of clodronate liposomes and anti-ASGM1 antibody depletes Kupffer cells and NK cells, respectively.
  • Fig. S7. Clodronate liposomes inhibit poly I:C–induced depletion of c-FLIP protein in the livers of c-FlipF/F;Mx1-Cre mice through suppression of Ifnb1 expression.
  • Table S1. Elimination of Tnfr1 partially rescues the perinatal lethality of c-FlipF/F;Villin-Cre mice.
  • Table S2. Genotyping of c-FlipF/F;Alfp-Cre mice that were generated by crossing c-FlipF/+;Alfp-Cre mice with c-FlipF/F mice.
  • Table S3. Elimination of Tnfr1 does not rescue the perinatal lethality of c-FlipF/F;Alfp-Cre mice.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1 MB


Citation: X. Piao, S. Komazawa-Sakon, T. Nishina, M. Koike, J.-H. Piao, H. Ehlken, H. Kurihara, M. Hara, N. Van Rooijen, G. Schütz, M. Ohmuraya, Y. Uchiyama, H. Yagita, K. Okumura, Y.-W. He, H. Nakano, c-FLIP Maintains Tissue Homeostasis by Preventing Apoptosis and Programmed Necrosis. Sci. Signal. 5, ra93 (2012).

© 2012 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882