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Sci. Signal., 12 February 2013
[DOI: 10.1126/scisignal.2003417]

Supplementary Materials for:

A Nontranscriptional Role for HIF-1α as a Direct Inhibitor of DNA Replication

Maimon E. Hubbi, Kshitiz, Daniele M. Gilkes, Sergio Rey, Carmen C. Wong, Weibo Luo, Deok-Ho Kim, Chi V. Dang, Andre Levchenko, Gregg L. Semenza*

*To whom correspondence should be addressed. E-mail: gsemenza{at}

This PDF file includes:

  • Fig. S1. Flow cytometry data analyzed in Fig. 1.
  • Fig. S2. Cdc6 has no effect on HIF-1α protein abundance or HIF-1 activity.
  • Fig. S3. Cell cycle arrest in response to hypoxia.
  • Fig. S4. HIF-1α promotes MCM loading in HeLa and NIH-3T3 cells.
  • Fig. S5. Hypoxia decreases total Cdc6 abundance.
  • Fig. S6. HIF-1β knockdown cells have decreased HIF-1 transcriptional activity.

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Format: Adobe Acrobat PDF

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Citation: M. E. Hubbi, Kshitiz, D. M. Gilkes, S. Rey, C. C. Wong, W. Luo, D.-H. Kim, C. V. Dang, A. Levchenko, G. L. Semenza, A Nontranscriptional Role for HIF-1α as a Direct Inhibitor of DNA Replication. Sci. Signal. 6, ra10 (2013).

© 2013 American Association for the Advancement of Science

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