A Nontranscriptional Role for HIF-1α as a Direct Inhibitor of DNA Replication

Maimon E. Hubbi, Kshitiz, Daniele M. Gilkes, Sergio Rey, Carmen C. Wong, Weibo Luo, Deok-Ho Kim, Chi V. Dang, Andre Levchenko, Gregg L. Semenza*

*To whom correspondence should be addressed. E-mail: gsemenza{at}jhmi.edu

This PDF file includes:

• Fig. S1. Flow cytometry data analyzed in Fig. 1.
• Fig. S2. Cdc6 has no effect on HIF-1α protein abundance or HIF-1 activity.
• Fig. S3. Cell cycle arrest in response to hypoxia.
• Fig. S4. HIF-1α promotes MCM loading in HeLa and NIH-3T3 cells.
• Fig. S5. Hypoxia decreases total Cdc6 abundance.
• Fig. S6. HIF-1β knockdown cells have decreased HIF-1 transcriptional activity.

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© 2013 American Association for the Advancement of Science