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Science 316 (5829): 1345-1348

Copyright © 2007 by the American Association for the Advancement of Science

Complex I Binding by a Virally Encoded RNA Regulates Mitochondria-Induced Cell Death

Matthew B. Reeves,1* Andrew A. Davies,1 Brian P. McSharry,2 Gavin W. Wilkinson,2 John H. Sinclair1{dagger}

Abstract: Human cytomegalovirus infection perturbs multiple cellular processes that could promote the release of proapoptotic stimuli. Consequently, it encodes mechanisms to prevent cell death during infection. Using rotenone, a potent inhibitor of the mitochondrial enzyme complex I (reduced nicotinamide adenine dinucleotide–ubiquinone oxido-reductase), we found that human cytomegalovirus infection protected cells from rotenone-induced apoptosis, a protection mediated by a 2.7-kilobase virally encoded RNA (ß2.7). During infection, ß2.7 RNA interacted with complex I and prevented the relocalization of the essential subunit genes associated with retinoid/interferon–induced mortality–19, in response to apoptotic stimuli. This interaction, which is important for stabilizing the mitochondrial membrane potential, resulted in continued adenosine triphosphate production, which is critical for the successful completion of the virus' life cycle. Complex I targeting by a viral RNA represents a refined strategy to modulate the metabolic viability of the infected host cell.

1 Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Hills Road, Cambridge, CB2 2QQ, UK.
2 Section for Infection and Immunity, College of Medicine, University of Wales, Heath Park, Cardiff, CF14 4XX, UK.

* Present address: Novartis Institutes for Biomedical Research, 500 Technology Square, Cambridge, MA 02139, USA. Back

{dagger} To whom correspondence should be addressed. E-mail: js{at}

During primary infection or reactivation of human cytomegalovirus (HCMV), especially in the immunocompromised, the virus is able to replicate in a number of cell types, often resulting in life-threatening disease (1). HCMV exhibits a relatively protracted life cycle (upwards of 5 days) and at early times of infection (12 to 24 hours) encodes a highly abundant 2.7-kb RNA transcript (ß2.7), accounting for >20% of total viral gene transcription (2, 3) of unknown function. The RNA may be associated with mitochondria (4), and no protein product of this RNA has ever been detected in infected cells (3), suggesting that it functions as a noncoding RNA (5).

We investigated the possibility that ß2.7 could function as a noncoding RNA. A Northwestern screen of a human cDNA library with a ß2.7 probe identified potential cellular interaction partners for the ß2.7 RNA molecule. One of these proteins was a subunit of the mitochondrial enzyme complex I (reduced nicotinamide adenine dinucleotide–ubiquinone oxido-reductase). Defective complex I activity has been implicated in numerous mitochondrial and genetic diseases, including Leigh's syndrome, Leber's hereditary optic neuropathy, and mitochondrial encephalopathy (6); and inhibition of complex I activity by reactive O or N species or by the direct binding of environmental toxins ultimately results in apoptosis (7, 8).

We first tested whether HCMV infection [multiplicity of infection (MOI) = 5] and, specifically, ß2.7 expression prevented cell death in neuronal U373 cells subjected to mitochondrial stress by treatment with rotenone—a highly effective complex I inhibitor (9, 10). As expected, the addition of rotenone promoted substantial cell death in U373 cells (70% in Fig. 1). We then compared the effect of preinfection of cells with the Toledo strain of HCMV and a recombinant Toledo virus, in which the ß2.7 gene had been deleted ({Delta}ß2.7Tol) (5). Toledo-infected U373 cultures showed profoundly (P < 0.001) reduced levels of apoptosis (3%), in contrast to {Delta}ß2.7Tol-infected cells (70% in Fig. 1A). Furthermore, the protective effect of ß2.7 could be restored with a revertant virus (Fig. 1A) and by transfection of a ß2.7 expression vector into U373 cells (Fig. 1B). Thus, HCMV-mediated protection of cells from rotenone-induced apoptosis correlated with expression of the viral ß2.7 gene. Although the consensus is that the ß2.7 transcript does not encode a protein product (5), we also analyzed a clinical isolate of HCMV (HCMV-3157), which would produce a heavily truncated nonsense protein if ß2.7 were translated (5). HCMV-3157 was as efficient as Toledo at protecting cells from rotenone-induced death (fig. S1A). The protective effect of ß2.7 was observed only in Toledo-infected cells (fig. S1B), whereas {Delta}ß2.7Tol-infected cells routinely stained for both viral gene expression and terminal deoxynucleotidyl transferase–mediated deoxyuridine triphosphate nick end labeling (TUNEL) (fig. S1B). The proportion of immediate-early (IE)–positivity in the {Delta}ß2.7Tol cells undergoing apoptosis was around 70% (fig. S1C). Given that only 45% of the whole population was infected, this finding implied that viral infection, in the absence of ß2.7, actually rendered cells more sensitive to rotenone-induced apoptosis.

Figure 1 Fig. 1.. HCMV protects cells from rotenone-induced apoptosis. (A) Percentage of TUNEL-positive cells in mock- (1 and 2), Toledo- (3 and 4), {Delta}ß2.7Tol- (5 and 6), and {Delta}ß2.7Tol-Revertant ({Delta}ß2.7Tol-Rev)–infected (7 and 8) U373 cells incubated with acetone (1, 3, 5, and 7) or rotenone (ROT) (2, 4, 6, and 8). n = 3 independent experiments. Asterisks denote P < 0.01 with Student's t test. Error bars indicate 1 SEM. (B) Percentage of TUNEL-positive pREP10- (Con) (1) and pREP10-ß 2.7–transfected (2) cells incubated with rotenone. from three independent analyses. Reverse transcription (RT)–PCR for ß2.7 expression in pREP10- (lane 1) and pREP10-ß2.7–transfected (lane 2) cells is shown. [View Larger Version of this Image (17K GIF file)]

One role of the HCMV ß2.7 transcript may be to mediate protection of the cell from apoptotic pathways activated by metabolic stress of complex I. Genes associated with retinoid/interferon–induced mortality (GRIM)–19 is a subunit of complex I that is essential for its assembly and function (11). HCMV infection up-regulates steady-state mRNA levels of some subunits of mitochondrial complexes I to V (12), but virus infection has no impact on GRIM-19 protein expression, up to 120 hours post infection (hpi) in U373 cells (fig. S2, A and B). We did, however, detect changes in GRIM-19 localization in response to rotenone and virus infection. In U373 cells, GRIM-19 expression appeared to be diffuse throughout the cytoplasm of the cell (Fig. 2A), and it became relocalized into discrete perinuclear clumps after the addition of rotenone to uninfected U373 cells. However, preinfection with Toledo prevented this relocalization (Fig. 2A). GRIM-19 is known to localize to the nucleus under certain conditions (13), and it also interacts with the signal transducer and activator of transcription (STAT)–3 protein to prevent the nuclear import of STAT-3 in a perinuclear location (14). Although the GRIM-19 in Toledo-infected U373 remained predominantly cytoplasmic within the cell, it did exhibit a more punctuate pattern within the cytoplasm (Fig. 2A), perhaps suggesting more association with mitochondria. However, HCMV itself has profound effects on mitochondrial shape and localization after infection (15), and this may also partially account for the difference in GRIM-19 staining. In contrast, in {Delta}ß2.7Tol-infected cells, the rotenone-induced perinuclear relocalization of the GRIM-19 protein was still observed (Fig. 2A), identical to that observed with uninfected cells treated with rotenone (d in Fig. 2A). Thus, rotenone-induced mitochondrial stress promotes the relocalization of GRIM-19 within the cell, which can be abrogated by expression of the HCMV ß2.7 transcript.

Figure 2 Fig. 2.. HCMV interacts with and prevents the rotenone-induced relocalization of, GRIM-19 in virally infected cells. (A) GRIM-19 localization (red) in mock- (a and d), Toledo- (b and e), and {Delta}ß2.7Tol-infected (c and f) U373 cells incubated with solvent control (Con) (a, c, and e) or rotenone (b, d, and f) 24 hpi. (B to D) Immunoprecipitation of control immunoglobulin G (lane 2), GRIM-19 (G-19) (lane 3), native complex I (C-1) (lane 4), and native complex V (C-V) (lane 5) from Toledo- (B and C) or {Delta}ß2.7Tol-infected (D) cells, and RT-PCR for ß2.7 [(B) and (D), lanes 1 to 5], IE72 (C), or a ß2.7 PCR with no prior RT [(B), lanes 6 to 10]. Inputs are shown in lane 1. (E) IPs on Toledo-infected cells with anti-sense oligonucleotides to ß2.7 (lane 3) or IE72 (lane 4) or with no oligonucleotide (N) (lane 2). Silver stain of 1% input (INP) (lane 1), the immunoprecipitated proteins (lanes 2 to 4), and known protein loading controls (5 to 500 ng, lanes 5 to 7) is shown. (F) GRIM-19 expression in 10% input (lane 1) and the ß2.7 (lane 2) and IE72 (lane 3) RNA-IP samples. [View Larger Version of this Image (27K GIF file)]

We next tested whether this effect on GRIM-19 relocalization was due to a physical interaction between the GRIM-19 protein and ß2.7 RNA during infection. ß2.7 RNA was specifically immunoprecipitated from infected cells with An anitbody to GRIM-19 (Fig. 2B). We also observed an interaction with native complex I, which is found only on the inner mitochondrial membrane, suggesting that the interaction between ß2.7 RNA and GRIM-19 targets complex I in mitochondria. No immunoprecipitation of a similarly abundant viral RNA (IE72 in Fig. 2, B and C) with GRIM-19, complex I, or complex V (Fig. 2C) was observed. As expected, analyses using the {Delta}ß2.7Tol virus showed no ß2.7-specific polymerase chain reaction (PCR) band (Fig. 2D).

In a reciprocal analysis, we captured ß2.7 RNA with the use of biotin-labeled oligonucleotide probes (Fig. 2E). Immunoprecipitations (IPs) of the ß2.7-captured complexes did contain GRIM-19 (Fig. 2F). Thus, ß2.7 RNA specifically interacts with GRIM-19, but with few other proteins in vivo.

The observed physical interaction with GRIM-19 and complex I was investigated further. Active complex I supports the formation of an electrochemical gradient ({Delta}{psi}M) across the inner mitochondrial membrane, which is imperative for the efficient production of adenosine triphosphate (ATP) (16). Expression of ß2.7 RNA during infection or transfection protected {Delta}{psi}M stability from rotenone (fig. S3, A and B), suggesting that the ß2.7 RNA interaction with complex I could affect mitochondrial energy production under oxidative stress after infection. Previous data have shown that, unlike in herpes simplex virus (17), ATP levels in HCMV-infected cells are maintained at 24 hpi (18), a requirement probably attributable to HCMVs' comparatively protracted growth phase (19). We therefore analyzed the role of ß2.7 in ATP production.

Rotenone substantially reduced ATP production in U373 cells (75% reduction, 1 and 2 in Fig. 3A). However, in Toledo-infected cells, only a 1.2-fold reduction was observed (84 to 67% in Fig. 3A), suggesting that HCMV protected ATP production in infected cells. In {Delta}ß2.7Tol-infected cells, rotenone treatment resulted in a 2.5-fold depletion of intracellular ATP (77 to 33% in Fig. 3A). At 6 hpi, before ß2.7 RNA expression, no protection from rotenone-induced ATP depletion occurred (fig. S4). Using a second strain of HCMV AD169 that encodes two copies of the ß2.7 gene, we eliminated the possibility that green fluorescent protein (GFP) expression from the ß2.7 gene locus would have a phenotypic effect, because a recombinant AD169 virus still expressing GFP and one functional copy of the ß2.7 gene maintained ATP levels (Fig. 3A). Thus, the ß2.7 transcript is important for maintaining ATP production.

Figure 3 Fig. 3.. ß2.7 expression maintains ATP production in infected cells. (A) Percentage of ATP content of mock- (1 and 2), {Delta}ß2.7Tol- (3 and 4), Toledo- (Tol) (5 and 6), AD169-, and AD169-GFP–infected cells with (2,4, 6,8, and 10) or without (1, 3, 5, 7, and 9) rotenone for 2 hours was determined at 48 hpi, as compared to mock (1). (B) Percentage of ATP in mock-(1), {Delta}ß2.7Tol- (2), Toledo- (3), AD169- (4), or AD169-GFP–infected cells (5) 5 dpi. n = 3 independent experiments. Asterisks denote P < 0.01 with Student's t test. Error bars indicate 1 SEM. [View Larger Version of this Image (14K GIF file)]

We hypothesized that the impact of ß2.7 expression on ATP production may be more profound at later times of infection. Analysis in the absence of rotenone showed that ATP levels in {Delta}ß2.7Tol dropped significantly (P < 0.01) at 5 days post infection (dpi), in direct contrast with Toledo-infected cells. Confirmation that the difference was not an artifact of GFP production was performed with the AD169-GFP–infected cells, in which the levels of ATP were comparable to those of the parent virus AD169 (Fig. 3B).

Deletion of the viral ß2.7 gene from HCMV has no significant effects on growth kinetics in fibroblasts (5) (Fig. 4A). At first, this appears to be contradictory; however, fibroblasts are particularly resistant to the induction of apoptosis by rotenone and oxidative stress (20), as compared with neuronal cells (21). However, a {Delta}ß2.7Tol growth defect was observed when compared with the Toledo virus (Fig. 4B), which was more profound in the presence of rotenone (Fig. 4B), as is entirely consistent with the ß2.7 transcript supporting virus production in times of metabolic stress. Tissue culture was performed in glucose-enriched media, and fibroblasts, particularly in times of diminished ATP production from the electron transport chain (ETC), use this additional glucose to generate ATP via alternate pathways (22). We observed that glucose-depleted media impaired the growth of the {Delta}ß2.7Tol virus in U373 and fibroblast cells, as compared with Toledo (Fig. 4C). This finding correlated with a drop in ATP production in {Delta}ß2.7Tol-infected fibroblasts (fig. S5A) and the relocalization of GRIM-19 in {Delta}ß2.7Tol-infected, but not Toledo-infected, cells (fig. S5B). Because cells in vivo are not exposed to such artificially high levels of glucose and are more reliant on the ETC for ATP production, the effects of ß2.7 expression in vivo on metabolism are probably more overt than they are during tissue culture.

Figure 4 Fig. 4.. Toledo, but not {Delta}ß2.7Tol, grows normally in metabolically stressed cells. (A) Growth of Toledo (triangle), AD169 (diamond), AD169-GFP (square), or {Delta}ß2.7Tol (cross) in human fibroblasts (HFs). PFU, plaque-forming units. (B) Growth of Toledo (diamond and triangle) or {Delta}ß2.7Tol (square and cross) in U373 cells. At 24 hpi, Toledo-infected (triangle) and {Delta}ß2.7Tol-infected (cross) U373 cells were incubated with rotenone (asterisk). (C) Growth of Toledo (square and cross) or {Delta}ß2.7Tol (diamond or triangle) in U373 and HF cells in glucose-depleted media. [View Larger Version of this Image (20K GIF file)]

Metabolic dysfunction, the breakdown of mitochondrial integrity, and the release of proapoptotic stimuli are hallmarks of mitochondria-induced apoptosis (23). HIV-1 targets complex I for degradation promoting apoptosis (24), presumably by promoting the formation of reactive oxidative species. Because of the pivotal nature of mitochondria in cell death, it is not surprising that HCMV targets mitochondria function and makes a concerted effort to subvert the apoptotic response including UL36 (caspase inhibitor), UL37x1 (a B cell lymphoma 2 homolog with profound anti-apoptotic activity) (25, 26), and UL38, which protects infected cells from endoplasmic reticulum stress (27). UL37x1 is also known to promote mitochondrial membrane stability (26) and is predominantly active at late times of infection (>48 hpi) (28). In contrast, the ß2.7 gene RNA is expressed much earlier during infection. Consequently, it is likely that HCMV has evolved multiple functions in order to hijack the mitochondria in the cell to enable continued energy production, as well as protection from cell death.

An intriguing aspect of this study is that an RNA molecule is used directly to exert these effects. Although at first appearing unusual, this may represent a highly refined viral strategy. First, because of the sheer numbers of mitochondria within the cell, the expression of a highly abundant RNA allows the virus to saturate these organelles effectively. Second, by disposing of the need to translate the superabundant ß2.7 RNA, the virus can achieve this effect more quickly throughout the course of infection.

References and Notes Back to Top

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Supporting Online Material

Materials and Methods

Figs. S1 to S5


Received for publication 26 March 2007. Accepted for publication 23 April 2007.

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M. M. Monick, L. S. Powers, C. W. Barrett, S. Hinde, A. Ashare, D. J. Groskreutz, T. Nyunoya, M. Coleman, D. R. Spitz, and G. W. Hunninghake (2008)
J. Immunol. 180, 7485-7496
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Murine Cytomegalovirus m38.5 Protein Inhibits Bax-Mediated Cell Death.
I. Jurak, U. Schumacher, H. Simic, S. Voigt, and W. Brune (2008)
J. Virol. 82, 4812-4822
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GRIM-19 Is Essential for Maintenance of Mitochondrial Membrane Potential.
H. Lu and X. Cao (2008)
Mol. Biol. Cell 19, 1893-1902
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Modulation of Oncogenic Phenotype in Human Glioma Cells by Cytomegalovirus IE1-Mediated Mitogenicity.
C. S. Cobbs, L. Soroceanu, S. Denham, W. Zhang, and M. H. Kraus (2008)
Cancer Res. 68, 724-730
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Discrete Clusters of Virus-Encoded MicroRNAs Are Associated with Complementary Strands of the Genome and the 7.2-Kilobase Stable Intron in Murine Cytomegalovirus.
A. H. Buck, J. Santoyo-Lopez, K. A. Robertson, D. S. Kumar, M. Reczko, and P. Ghazal (2007)
J. Virol. 81, 13761-13770
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Antisense Transcription in the Human Cytomegalovirus Transcriptome.
G. Zhang, B. Raghavan, M. Kotur, J. Cheatham, D. Sedmak, C. Cook, J. Waldman, and J. Trgovcich (2007)
J. Virol. 81, 11267-11281
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IMMUNOLOGY: Outwitted by Viral RNAs.
B. R. Cullen (2007)
Science 317, 329-330
   Abstract »    Full Text »    PDF »

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