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Science 317 (5839): 803-806

Copyright © 2007 by the American Association for the Advancement of Science

Augmented Wnt Signaling in a Mammalian Model of Accelerated Aging

Hongjun Liu,1 Maria M Fergusson,1* Rogerio M. Castilho,2* Jie Liu,1 Liu Cao,1 Jichun Chen,3 Daniela Malide,4 Ilsa I. Rovira,1 Daniel Schimel,5 Calvin J. Kuo,6 J. Silvio Gutkind,2 Paul M. Hwang,1 Toren Finkel1{dagger}

Abstract: The contribution of stem and progenitor cell dysfunction and depletion in normal aging remains incompletely understood. We explored this concept in the Klotho mouse model of accelerated aging. Analysis of various tissues and organs from young Klotho mice revealed a decrease in stem cell number and an increase in progenitor cell senescence. Because klotho is a secreted protein, we postulated that klotho might interact with other soluble mediators of stem cells. We found that klotho bound to various Wnt family members. In a cell culture model, the Wnt-klotho interaction resulted in the suppression of Wnt biological activity. Tissues and organs from klotho-deficient animals showed evidence of increased Wnt signaling, and ectopic expression of klotho antagonized the activity of endogenous and exogenous Wnt. Both in vitro and in vivo, continuous Wnt exposure triggered accelerated cellular senescence. Thus, klotho appears to be a secreted Wnt antagonist and Wnt proteins have an unexpected role in mammalian aging.

1 Cardiology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
2 Oral and Pharyngeal Cancer Branch, National Institute of Dental and Craniofacial Research, NIH, Bethesda, MD 20892, USA.
3 Hematology Branch, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
4 Light Microscopy Core Facility, National Heart, Lung, and Blood Institute, NIH, Bethesda, MD 20892, USA.
5 Mouse Imaging Facility, NIH, Bethesda, MD 20892, USA.
6 Division of Hematology, Stanford University School of Medicine, Stanford, CA 94305, USA.

* These authors contributed equally to this work. Back

{dagger} To whom correspondence should be addressed. E-mail: finkelt{at}

Resident and circulating stem and progenitor cells are critical for ongoing tissue maintenance and repair, and it is often postulated that stem and progenitor cell depletion or dysfunction might contribute to aging (1). We therefore examined stem cell dynamics in a genetic model of accelerated aging. Mice lacking klotho expression, henceforth termed Klotho mice, have a shortened life span and exhibit a number of early-onset age-related changes, including arteriosclerosis, decreased fertility, and skin atrophy (2). Klotho is a transmembrane protein with a large extracellular domain composed of two repeats (KL1 and KL2 domains) that share similarity to Family I glycosidases. In addition to being cell-associated, the extracellular portion of klotho is secreted and can be detected in the circulation of animals and humans (3). It is generally believed that secreted klotho is the form most likely mediating the protein's longevity effects.

One alteration in Klotho animals is the early appearance of age-related changes in the skin. To assess whether these phenotypic changes were accompanied by alterations in stem cell number, we identified the number of long-term 5-bromo-2'-deoxyuridine (BrdU)–retaining cells in the skin of either wild-type or age-matched Klotho animals (4). These label-retaining cells (LRCs) are a convenient method to identify stem cells within their niche (5). At an age of 2.5 months, Klotho mice had significantly fewer LRCs than their wild-type littermates [wild type: 80 ± 5 LRCs (±SD) per set of three follicles versus Klotho: 35 ± 3 LRCs, n = 30, P <0.05 paired t test]. Skin LRCs are confined to a specialized region of the follicle known as the bulge region, and the stem cells contained within this niche are enriched for CD34 expression (6). The bulge region in Klotho animals was consistently smaller with reduced CD34 expression (Fig. 1A). Hair follicle epidermal stem cells are also a source of transient amplifying (TA) cells induced by acute wounding (7). Consistent with a defect in the number of LRCs, epidermal wounding resulted in a diminished number of TA cells in the Klotho animals (Fig. 1B) and a deficit in wound closure (Fig. 1C).

Figure 1 Fig. 1.. Altered stem and progenitor cells in Klotho mice. (A) LRCs within the skin follicles of 80-day-old wild-type (WT) or Klotho (Klo) mice (top). Higher magnification of representative bulge regions stained for CD34 (bottom). (B) TA cells, identified by positive brown nuclear BrdU staining, after skin wounding. (C) Assessment of skin closure 4 days after creating a 1-cm wound (n = 4 pairs, *P < 0.05 paired t test). (D) Evidence for senescence within the hair follicle of Klotho animals as assessed by ß-gal staining (SAß-gal) and nuclear foci of {gamma}-H2AX and 53BP1 (red) in 4',6'-diamidino-2-phenylindole (DAPI)–stained nuclei (blue). Percentage of positive nuclear staining in either wild-type or Klotho follicles is shown ± SD. (E) SAß-gal staining of small intestine. (F) Determination of the absolute number of c-kit+sca-1+ Lin HSCs in bilateral femur and tibias of either wild-type or Klotho animals (n = 3 pairs, *P < 0.05 paired t test). (G) Representative cell cycle analysis of HSCs from wild-type and Klotho animals demonstrating a decrease in HSC quiescence (% G0) and increased proliferation (% G1). DNA content is displayed along the x axis; RNA content determined by Pyronin Y (PY) staining is displayed along the y axis. [View Larger Version of this Image (57K GIF file)]

Age-matched wild-type and Klotho skin sections also exhibited differences in senescence-associated endogenous ß-galactosidase (SAß-gal) activity (Fig. 1D). The observed SAß-gal staining occurred in the outermost epidermal layer including the acellular stratum corneum. The specificity and physiological significance of this staining is unclear. Examination of numerous random follicles revealed that the Klotho animals also had intense ß-galactosidase staining within the follicles, especially within regions known to contain rapidly dividing progenitor cells (8). We observed little to no SAß-gal staining in the intrafollicular regions. Senescent cells often activate the DNA damage response (DDR) pathway, as evidenced by the development of nuclear foci of proteins such as phosphorylated histone (H2AX), ataxia telangiectasia mutated (ATM), and binding protein 1 (53BP1) (9). The DDR pathway was activated in multiple random Klotho follicles but not in age-matched wild-type mice (Fig. 1D).

Klotho animals also demonstrated increased SAß-gal staining in the small intestine, especially within intestinal crypts, an area enriched for stem and progenitor cells (Fig. 1E). Similar analysis of the testis in male animals also demonstrated evidence of increased progenitor cell senescence (fig. S1). In the bone marrow of Klotho mice, there was also a reduction in the population of cells bearing the cell surface phenotype of c-kit+ sca-1+ lineage negative that encompasses the hematopoietic stem cell (HSC) (Fig. 1F). This reduction of HSC in Klotho animals was accompanied by a marked increase in the percentage of stem cells that were actively dividing (Klotho HSCs: 28.4 ± 3.7% in G1 versus wild-type HSCs: 10.2 ± 1.1% in G1, n = 3 animal pairs per group, P <0.05 paired t test) (Fig. 1G).

Given that stem cell biology is regulated by a number of secreted factors, we wondered whether there might be a functional interaction between klotho and one of these known stem cell regulators. In the course of our experiments, we noted that the subcellular distribution of klotho and Wnt proteins within transfected cells overlapped (fig. S2). Thus, we sought to determine whether klotho and Wnt3 could form a direct molecular complex. Epitope-tagged Wnt3 and myc-tagged klotho were readily detectable in transfected cell lysates (Fig. 2A). Klotho associated with immunoprecipitated Wnt3, and the reciprocal immunoprecipitation of klotho contained Wnt3 (Fig. 2A). A single extracellular KL domain was sufficient to mediate the observed interaction with Wnt3 (Fig. 2B). The Wnt binding domain was contained within the amino-terminal portion of klotho's KL1 domain (amino acids 1 to 285) (fig. S3). Full-length klotho also immunoprecipitated with a number of other Wnt isoforms including Wnt1, Wnt4, and Wnt5a (fig. S4).

Figure 2 Fig. 2.. Interaction of klotho with Wnt and inhibition of Wnt signaling. (A) HEK-293 cells were transiently transfected with myc- and hemagglutinin (HA)–tagged expression constructs encoding Wnt3 and murine klotho (Klo). Reciprocal coimmunoprecipitation (IP) of klotho and Wnt3 from cell lysates is also demonstrated. WB, Western blot. (B) Schematic diagram of klotho demonstrating the two extracellular klotho repeats (KL1 and KL2) followed by the single-pass transmembrane domain (–). Flag-tagged full-length klotho (Klo) and truncation mutants were assessed for Wnt binding. (C) HEK-293 cells were transfected with a Wnt3 expression construct (100 ng DNA) and the indicated amount of a full-length klotho expression vector along with either an active (TOPFlash) or inactive (FOPFlash) Wnt luciferase reporter. (D) Conditioned medium mixes were incubated for 24 hours with HEK-293 cells previously transfected with either the active or inactive Wnt luciferase reporter. All Wnt activity measurements represent the ratio of TOP/FOP activity obtained from a single experiment performed in triplicate and are representative of at least three similar experiments. [View Larger Version of this Image (30K GIF file)]

We further analyzed the biological effects of the observed klotho/Wnt interaction. We coexpressed Wnt3 and klotho in human embryonic kidney (HEK) 293 cells along with a Wnt-responsive reporter. The presence of Wnt3 increased reporter activity by approximately 7 times, whereas the addition of increasing amounts of klotho reduced Wnt activity in a dose-dependent fashion (Fig. 2C). Various structural mutants containing a single KL1 domain or altering conserved amino acids believed to be important in klotho's ß-glucuronidase activity failed to abrogate klotho-mediated Wnt inhibition (fig. S5). In contrast, klotho constructs that failed to physically interact with Wnt3 also failed to inhibit Wnt activity (fig. S6).

We next asked whether klotho could inhibit Wnt activity in a cell-free system. Conditioned media with and without either klotho or Wnt3a were prepared and mixed in various combinations before being placed on HEK-293 cells transfected with a Wnt reporter construct. These results indicated that secreted klotho could inhibit soluble Wnt activity (Fig. 2D). In contrast, secreted klotho-conditioned medium was ineffective in inhibiting Wnt signaling directly stimulated by intracellular ß-catenin expression (fig. S7).

We next tested whether animals lacking klotho expression had increased Wnt activity. We crossed Klotho animals with the TOPGAL reporter strain in which the activity of a ß-galactosidase reporter is under the control of Wnt-responsive elements (10). Analysis of the skin of Klotho/TOPGAL animals demonstrated an increase in ß-galactosidase reporter activity compared with that of age-matched wild-type/TOPGAL controls (Fig. 3A). Quantitative analysis confirmed this augmented Wnt activity in the skin and small intestine of Klotho mice and demonstrated that the increased expression of the Wnt-dependent ß-galactosidase reporter was similar to that of Axin2, a known Wnt target gene (fig. S8). Other potential transcriptional targets of the Wnt pathway also showed increased transcription in Klotho animals (fig. S9).

Figure 3 Fig. 3.. Inhibition of Wnt signaling by klotho in vivo. (A) Endogenous Wnt activity in skin from 14-day-old wild-type or Klotho mice crossed with the TOPGAL reporter strain. (B) Wnt activity in tibias of 14-day-old Klotho/TOPGAL or wild-type/TOPGAL mice. (C) Longitudinal microcomputerized CT sections from the tibia of 3-month-old Klotho (top) or wild-type (bottom) animals. (D) Horizontal three-dimensional reconstruction of cortical bone (yellow) and trabecular bone (green). (E) Calculation of the trabecular to total bone volume from the tibia of three pairs of 3-month-old wild-type or Klotho animals. (F) Quantification of the percentage of ß-galactosidase positive follicles from 12-day-old TOPGAL mice injected 4 days earlier with either a control virus (Ad.FC), an adenovirus encoding the Wnt inhibitor (Ad.DKK1), or an adenovirus encoding klotho (Ad.Klo). Approximately 500 random follicles were assessed per condition. (G) Hyperplastic skin phenotype of K5rtTA/tet-Wnt1 transgenic mice treated with doxycycline is blocked by prior injection of an adenovirus encoding for either DKK-1 or klotho. Mice were killed on postnatal day 21. [View Larger Version of this Image (51K GIF file)]

Whereas normal aging is associated with bone loss, studies in mice and humans have established that augmented Wnt signaling leads to increased bone mass (11). We therefore monitored Wnt activity in bones of aged-matched wild-type or Klotho littermates. Using the TOPGAL reporter, we detected augmented reporter activity in sections obtained from the proximal tibia of 2-week-old Klotho mice (Fig. 3B and fig. S10). At this time point, Klotho mice lack any discernible phenotype, suggesting that the increase in Wnt activity precedes the apparent onset of accelerated aging. We also analyzed by microcomputerized tomography (µCT) the tibias of 3-month-old wild-type or Klotho animals. Klotho mice demonstrated nearly 5 times as much tibial trabecular bone mass (Fig. 3, C to E). An increase in overall tibial and vertebral column trabecular bone density has actually been previously noted but unexplained in Klotho mice (12). In contrast, other bones in the Klotho animals exhibit decreased bone density (2). The basis for this regional difference in bone density is unclear but may relate to the complex interplay of klotho's ability both to alter Wnt signaling and to regulate other pathways involved in calcium and vitamin D homeostasis (1316).

To test whether augmented klotho expression could inhibit Wnt signaling in vivo, we analyzed TOPGAL reporter mice in the first few weeks of life, when the kinetics of the hair follicle cycle are synchronized (17). On postnatal day 8, mice were injected subcutaneously with one of three different recombinant adenoviruses encoding either klotho (Ad.Klo), the Wnt inhibitor DKK-1 (Ad.DKK1), or an adenovirus encoding the immunoglobulin G Fc fragment (Ad.FC) that served as a control. Four days after injection (postnatal day 12), we harvested the injected area and assessed Wnt activity in the hair follicle. In control-injected skin, Wnt activity was clearly visible around numerous growing hair follicles, whereas this activity was reduced in both the DKK-1 and klotho injected skin (fig. S11). Assessment of more than 500 random follicles in each condition revealed that klotho was roughly equivalent to DKK-1 in the ability to quantitatively suppress Wnt's biological activity (Fig. 3F).

To assess whether the expression of klotho could also block the effects of pathological Wnt expression, we crossed two strains of mice, one that expressed the tetracycline-inducible transcriptional activator (rtTA) under the control of the cytokeratin 5 promoter (K5rtTA) and another that expressed Wnt1 under the control of multiple tet-responsive elements (tet-Wnt1) (18, 19). The resultant cross generated the experimental line K5rtTA/tet-Wnt1, which provided tetracycline-inducible expression of Wnt1 in the basal layer of stratified epithelium. In our transgenic animals, ectopic expression of Wnt1 from postnatal day 12 to 21 resulted in increased epidermal thickness and marked follicular hyperplasia (Fig. 3G). The local injection of either DKK-1 or klotho, but not the control adenovirus, blocked these pathological Wnt-induced skin changes (Fig. 3G).

To begin to directly assess the role of Wnt proteins in aging, we tested whether continuous Wnt exposure induced cellular senescence. We grew primary mouse embryonic fibroblasts (MEFs) in the presence or absence of Wnt3a-conditioned medium. Consistent with Wnt proteins having mitogenic effects, analysis of BrdU incorporation revealed that Wnt-conditioned medium initially acted to increase MEF cell proliferation (Fig. 4A). However, over time, continuous Wnt exposure resulted in a marked decrease in proliferation (Fig. 4A and fig. S12). Wnt exposure did not increase the level of apoptosis (fig. S13); rather, assessment of cells grown in the continuous presence of Wnt3a demonstrated a flattened morphology with evidence of increased SAß-gal activity (Fig. 4B and fig. S14). Similarly, continuous Wnt3a exposure triggered the DDR pathway as well as nuclear foci of HP1-{alpha}, a marker of senescence-associated heterochromatin formation (Fig. 4C). The inhibitory effects of long-term Wnt3a-conditioned media on MEF proliferation (fig. S15) and BrdU incorporation (Fig. 4D) were attenuated by the addition of soluble klotho. Similar results were obtained with purified Wnt3a protein rather than Wnt3a-conditioned medium (Fig. 4E and fig. S16) and when using human rather than mouse cells (fig. S17).

Figure 4 Fig. 4.. Senescence induced by increased Wnt activity. (A) BrdU incorporation for MEFs grown in the presence or absence of Wnt3a conditioned media. Cells were assayed from passage 1 (P1) through passage 4 (P4). (B) Senescence associated ß-galactosidase staining (SAß-gal) for MEFs (P4) grown in standard media (–) or mixed with the indicated L-cell conditioned medium. (C) MEFs (P4) with or without continuous Wnt3a exposure were assessed for senescence-associated heterochromatin (HP1-{alpha}) and the activation of the DNA damage response. (D) Level of BrdU incorporation (P4) for MEF cells continuously treated with L-Wnt3a conditioned media (CM) mixed with either vector-transfected or klotho-transfected conditioned medium. (E) Growth of MEFs instandard medium (diamonds), or supplemented with Wnt3a at 10 ng/ml (squares) or 30 ng/ml (triangles). (Inset) P7 cells were stained for activation of H2AX. (F) Skin sections obtained from K5rtTA/tet-Wnt1 transgenic mice either treated or untreated with doxycycline (Dox) from postnatal days 2 through 21. The senescence-associated marker {gamma}-H2AX is induced in the setting of continuous in vivo Wnt1 (+Dox) exposure. [View Larger Version of this Image (50K GIF file)]

Finally, we tested whether Wnt could induce senescence in vivo by analyzing the skin of K5rtTA/tet-Wnt1 transgenic animals. Beginning on postnatal day 2, littermate transgenic animals were placed on a diet with or without doxycycline (Dox) and skin samples were collected for study on day 21. Analysis of multiple random follicles (n >100) demonstrated an increase in senescence markers in those animals exposed to continuous Wnt1 expression. On day 21, 70% of the follicles in Wnt1-expressing animals exhibited staining for {gamma}-H2AX, whereas less than 5% of uninduced follicles demonstrated this phenotype (Fig. 4F). Similarly, whereas follicles from young animals without Dox treatment showed little to no evidence of senescence, their Wnt1-induced littermates demonstrated multiple areas of discrete SAß-gal staining (fig. S18).

We demonstrate that klotho acts as a Wnt antagonist and that chronic Wnt stimulation may contribute to stem cell depletion and aging. During the course of our studies, two other studies appeared demonstrating that forced constitutive Wnt signaling within HSC led to a rapid exhaustion of long-term repopulating stem cells (20, 21). These results, as well as the observations in Brack et al. (22), are broadly consistent with our observations of Klotho mice. Previous attempts to understand the basis of the observed Klotho aging phenotype have implicated alterations in insulin signaling as well as, more recently, the fibroblast growth factor 23 pathway (1416, 23). Further analysis is therefore required to fully understand how these various klotho-regulated pathways potentially intersect and whether any biological hierarchy exists. Nonetheless, our results provide an unexpected connection between aging and the well-studied Wnt signaling pathway and suggest that strategies targeting soluble mediators of stem cell function may provide new therapeutic strategies to combat aging and potentially age-related diseases.

References and Notes Back to Top

  1. T. A. Rando, Nature 441, 1080 (2006).[CrossRef][Medline]
  2. M. Kuro-o et al., Nature 390, 45 (1997).[CrossRef][Medline]
  3. N. M. Xiao, Y. M. Zhang, Q. Zheng, J. Gu, Chin. Med. J. (Engl.) 117, 742 (2004).[Medline]
  4. Materials and methods are available as supporting material on Science Online.
  5. K. M. Braun, F. M. Watt, J. Invest. Dermatol. Symp. Proc. 9, 196 (2004).[CrossRef]
  6. T. Tumbar et al., Science 303, 359 (2004).[Abstract/Free Full Text]
  7. M. Ito et al., Nat. Med. 11, 1351 (2005).[CrossRef] [Web of Science][Medline]
  8. C. Blanpain, E. Fuchs, Annu. Rev. Cell Dev. Biol. 22, 339 (2006).[CrossRef] [Web of Science][Medline]
  9. D. B. Lombard et al., Cell 120, 497 (2005).[CrossRef] [Web of Science][Medline]
  10. R. DasGupta, E. Fuchs, Development 126, 4557 (1999).[Abstract]
  11. V. Krishnan, H. U. Bryant, O. A. Macdougald, J. Clin. Invest. 116, 1202 (2006).[CrossRef] [Web of Science][Medline]
  12. T. Yamashita, Y. Nabeshima, M. Noda, J. Endocrinol. 164, 239 (2000).[Abstract]
  13. Q. Chang et al., Science 310, 490 (2005).[Abstract/Free Full Text]
  14. H. Kurosu et al., J. Biol. Chem. 281, 6120 (2006).[Abstract/Free Full Text]
  15. M. S. Razzaque, D. Sitara, T. Taguchi, R. St-Arnaud, B. Lanske, FASEB J. 20, 720 (2006).[Abstract/Free Full Text]
  16. I. Urakawa et al., Nature 444, 770 (2006).[CrossRef][Medline]
  17. E. Fuchs, Nature 445, 834 (2007).[CrossRef][Medline]
  18. L. Vitale-Cross, P. Amornphimoltham, G. Fisher, A. A. Molinolo, J. S. Gutkind, Cancer Res. 64, 8804 (2004).[Abstract/Free Full Text]
  19. E. J. Gunther et al., Genes Dev. 17, 488 (2003).[Abstract/Free Full Text]
  20. M. Scheller et al., Nat. Immunol. 7, 1037 (2006).[CrossRef] [Web of Science][Medline]
  21. P. Kirstetter, K. Anderson, B. T. Porse, S. E. Jacobsen, C. Nerlov, Nat. Immunol. 7, 1048 (2006).[CrossRef] [Web of Science][Medline]
  22. A. S. Brack et al., Science 317, 807 (2007).[Abstract/Free Full Text]
  23. H. Kurosu et al., Science 309, 1829 (2005).[Abstract/Free Full Text]
  24. We thank R. Moon for the Wnt (TOP/FOP) reporter constructs, M. Kuro-o for generating the klotho mice, and H. Dietz for providing the klotho mice. The TOPGAL mouse was generated by E. Fuchs and kindly provided by Y. Yang. We also thank J. G. Kang for technical help. This work was supported by Intramural NIH funds (T.F.), a grant from the Ellison Medical Foundation (T.F.), and an NIH 1 R01 DK069989-01 to C.J.K.

Supporting Online Material

Materials and Methods

Figs. S1 to S18


Received for publication 9 April 2007. Accepted for publication 9 July 2007.

Aging increases CCN1 expression leading to muscle senescence.
J. Du, J. D. Klein, F. Hassounah, J. Zhang, C. Zhang, and X. H. Wang (2014)
Am J Physiol Cell Physiol 306, C28-C36
   Abstract »    Full Text »    PDF »
Biochemical and Functional Characterization of the Klotho-VS Polymorphism Implicated in Aging and Disease Risk.
T. B. Tucker Zhou, G. D. King, C. Chen, and C. R. Abraham (2013)
J. Biol. Chem. 288, 36302-36311
   Abstract »    Full Text »    PDF »
Growth hormone and Klotho.
C. Schmid, M. C. Neidert, O. Tschopp, L. Sze, and R. L. Bernays (2013)
J. Endocrinol. 219, R37-R57
   Abstract »    Full Text »    PDF »
Klotho Regulates Retinal Pigment Epithelial Functions and Protects Against Oxidative Stress.
M. Kokkinaki, M. Abu-Asab, N. Gunawardena, G. Ahern, M. Javidnia, J. Young, and N. Golestaneh (2013)
J. Neurosci. 33, 16346-16359
   Abstract »    Full Text »    PDF »
The Age-Regulating Protein Klotho Is Vital to Sustain Retinal Function.
N. J. Reish, A. Maltare, A. S. McKeown, A. M. Laszczyk, T. W. Kraft, A. K. Gross, and G. D. King (2013)
Invest. Ophthalmol. Vis. Sci. 54, 6675-6685
   Abstract »    Full Text »    PDF »
Molecular basis of organ fibrosis: Potential therapeutic approaches.
A. K. Ghosh, S. E. Quaggin, and D. E. Vaughan (2013)
Experimental Biology and Medicine 238, 461-481
   Abstract »    Full Text »    PDF »
Klotho to Treat Kidney Fibrosis.
M. D. Sanchez-Nino, A. B. Sanz, and A. Ortiz (2013)
J. Am. Soc. Nephrol. 24, 687-689
   Full Text »    PDF »
Loss of Klotho Contributes to Kidney Injury by Derepression of Wnt/{beta}-Catenin Signaling.
L. Zhou, Y. Li, D. Zhou, R. J. Tan, and Y. Liu (2013)
J. Am. Soc. Nephrol. 24, 771-785
   Abstract »    Full Text »    PDF »
Proliferation Rate of Somatic Cells Affects Reprogramming Efficiency.
Y. Xu, X. Wei, M. Wang, R. Zhang, Y. Fu, M. Xing, Q. Hua, and X. Xie (2013)
J. Biol. Chem. 288, 9767-9778
   Abstract »    Full Text »    PDF »
Telomere stability--Wnt it or lose it.
K. Bauer, S. Tao, and K. L. Rudolph (2013)
EMBO Rep. 14, 297-298
   Full Text »    PDF »
Oncogenic Wnt/{beta}-catenin signalling pathways in the cancer-resistant epididymis have implications for cancer research.
K. Wang, N. Li, C. H. Yeung, J. Y. Li, H. Y. Wang, and T. G. Cooper (2013)
Mol. Hum. Reprod. 19, 57-71
   Abstract »    Full Text »    PDF »
The Antiaging Protein Klotho Enhances Oligodendrocyte Maturation and Myelination of the CNS.
C.-D. Chen, J. A. Sloane, H. Li, N. Aytan, E. L. Giannaris, E. Zeldich, J. D. Hinman, A. Dedeoglu, D. L. Rosene, R. Bansal, et al. (2013)
J. Neurosci. 33, 1927-1939
   Abstract »    Full Text »    PDF »
FGF23 Suppresses Chondrocyte Proliferation in the Presence of Soluble {alpha}-Klotho both in Vitro and in Vivo.
M. Kawai, S. Kinoshita, A. Kimoto, Y. Hasegawa, K. Miyagawa, M. Yamazaki, Y. Ohata, K. Ozono, and T. Michigami (2013)
J. Biol. Chem. 288, 2414-2427
   Abstract »    Full Text »    PDF »
Satellite Cells and the Muscle Stem Cell Niche.
H. Yin, F. Price, and M. A. Rudnicki (2013)
Physiol Rev 93, 23-67
   Abstract »    Full Text »    PDF »
Klotho protects against mouse renal fibrosis by inhibiting Wnt signaling.
M. Satoh, H. Nagasu, Y. Morita, T. P. Yamaguchi, Y. S. Kanwar, and N. Kashihara (2012)
Am J Physiol Renal Physiol 303, F1641-F1651
   Abstract »    Full Text »    PDF »
Celebrating 30 Years of Wnt Signaling.
F. Verkaar, K. M. Cadigan, and R. van Amerongen (2012)
Science Signaling 5, mr2
   Abstract »    Full Text »    PDF »
Inducible progenitor-derived Wingless regulates adult midgut regeneration in Drosophila.
J. B. Cordero, R. K. Stefanatos, A. Scopelliti, M. Vidal, and O. J. Sansom (2012)
EMBO J. 31, 3901-3917
   Abstract »    Full Text »    PDF »
Wnt Proteins.
K. Willert and R. Nusse (2012)
Cold Spring Harb Perspect Biol 4, a007864
   Abstract »    Full Text »    PDF »
The emerging role of Klotho in clinical nephrology.
M. C. Hu, M. Kuro-o, and O. W. Moe (2012)
Nephrol. Dial. Transplant. 27, 2650-2657
   Abstract »    Full Text »    PDF »
Insulin-Like Growth Factors and Insulin: At the Crossroad Between Tumor Development and Longevity.
R. Novosyadlyy and D. LeRoith (2012)
J Gerontol A Biol Sci Med Sci 67A, 640-651
   Abstract »    Full Text »    PDF »
Short Hairpin RNA Screen Indicates That Klotho Beta/FGF19 Protein Overcomes Stasis in Human Colonic Epithelial Cells.
J. Kim, U. Eskiocak, G. Stadler, Z. Lou, M. Kuro-o, J. W. Shay, and W. E. Wright (2011)
J. Biol. Chem. 286, 43294-43300
   Abstract »    Full Text »    PDF »
Wnt5a Skews Dendritic Cell Differentiation to an Unconventional Phenotype with Tolerogenic Features.
J. Valencia, C. Hernandez-Lopez, V. G. Martinez, L. Hidalgo, A. G. Zapata, A. Vicente, A. Varas, and R. Sacedon (2011)
J. Immunol. 187, 4129-4139
   Abstract »    Full Text »    PDF »
The Inflammatory Cytokines TWEAK and TNF{alpha} Reduce Renal Klotho Expression through NF{kappa}B.
J. A. Moreno, M. C. Izquierdo, M. D. Sanchez-Nino, B. Suarez-Alvarez, C. Lopez-Larrea, A. Jakubowski, J. Blanco, R. Ramirez, R. Selgas, M. Ruiz-Ortega, et al. (2011)
J. Am. Soc. Nephrol. 22, 1315-1325
   Abstract »    Full Text »    PDF »
The Dual Role of Sirtuins in Cancer.
L. Bosch-Presegue and A. Vaquero (2011)
Genes & Cancer 2, 648-662
   Abstract »    Full Text »    PDF »
Manifestations and mechanisms of stem cell aging.
L. Liu and T. A. Rando (2011)
J. Cell Biol. 193, 257-266
   Abstract »    Full Text »    PDF »
Klotho Inhibits Transforming Growth Factor-{beta}1 (TGF-{beta}1) Signaling and Suppresses Renal Fibrosis and Cancer Metastasis in Mice.
S. Doi, Y. Zou, O. Togao, J. V. Pastor, G. B. John, L. Wang, K. Shiizaki, R. Gotschall, S. Schiavi, N. Yorioka, et al. (2011)
J. Biol. Chem. 286, 8655-8665
   Abstract »    Full Text »    PDF »
Four faces of cellular senescence.
F. Rodier and J. Campisi (2011)
J. Cell Biol. 192, 547-556
   Abstract »    Full Text »    PDF »
Wnt Signaling Regulates Hepatic Metabolism.
H. Liu, M. M. Fergusson, J. J. Wu, I. I. Rovira, J. Liu, O. Gavrilova, T. Lu, J. Bao, D. Han, M. N. Sack, et al. (2011)
Science Signaling 4, ra6
   Abstract »    Full Text »    PDF »
Wnt Signaling and Aging-Related Heart Disorders.
A. T. Naito, I. Shiojima, and I. Komuro (2010)
Circ. Res. 107, 1295-1303
   Abstract »    Full Text »    PDF »
A muscle resident cell population promotes fibrosis in hindlimb skeletal muscles of mdx mice through the Wnt canonical pathway.
F. Trensz, S. Haroun, A. Cloutier, M. V. Richter, and G. Grenier (2010)
Am J Physiol Cell Physiol 299, C939-C947
   Abstract »    Full Text »    PDF »
Cell Senescence in the Aging Kidney.
H. Yang and A. B. Fogo (2010)
J. Am. Soc. Nephrol. 21, 1436-1439
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Loss of Kitlow progenitors, reduced stem cell factor and high oxidative stress underlie gastric dysfunction in progeric mice.
F. Izbeki, D. T. Asuzu, A. Lorincz, M. R. Bardsley, L. N. Popko, K. M. Choi, D. L. Young, Y. Hayashi, D. R. Linden, M. Kuro-o, et al. (2010)
J. Physiol. 588, 3101-3117
   Abstract »    Full Text »    PDF »
Wnt signaling regulates mitochondrial physiology and insulin sensitivity.
J. C. Yoon, A. Ng, B. H. Kim, A. Bianco, R. J. Xavier, and S. J. Elledge (2010)
Genes & Dev. 24, 1507-1518
   Abstract »    Full Text »    PDF »
Targeting Wnt Signaling: Can We Safely Eradicate Cancer Stem Cells?.
F. Takahashi-Yanaga and M. Kahn (2010)
Clin. Cancer Res. 16, 3153-3162
   Abstract »    Full Text »    PDF »
The Apcmin mouse has altered hematopoietic stem cell function and provides a model for MPD/MDS.
S. W. Lane, S. M. Sykes, F. Al-Shahrour, S. Shterental, M. Paktinat, C. Lo Celso, J. L. Jesneck, B. L. Ebert, D. A. Williams, and D. G. Gilliland (2010)
Blood 115, 3489-3497
   Abstract »    Full Text »    PDF »
H. Sugiura, T. Yoshida, K. Nitta, and K. Tsuchiya (2010)
Nephrol. Dial. Transplant. 25, 1008-1009
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WNT16B Is a New Marker of Cellular Senescence That Regulates p53 Activity and the Phosphoinositide 3-Kinase/AKT Pathway.
R. Binet, D. Ythier, A. I. Robles, M. Collado, D. Larrieu, C. Fonti, E. Brambilla, C. Brambilla, M. Serrano, C. C. Harris, et al. (2009)
Cancer Res. 69, 9183-9191
   Abstract »    Full Text »    PDF »
Senescence, apoptosis, and stem cell biology: the rationale for an expanded view of intracrine action.
R. N. Re and J. L. Cook (2009)
Am J Physiol Heart Circ Physiol 297, H893-H901
   Abstract »    Full Text »    PDF »
Systems biology of autosomal dominant polycystic kidney disease (ADPKD): computational identification of gene expression pathways and integrated regulatory networks.
X. Song, V. Di Giovanni, N. He, K. Wang, A. Ingram, N. D. Rosenblum, and Y. Pei (2009)
Hum. Mol. Genet. 18, 2328-2343
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Axin expression in thymic stromal cells contributes to an age-related increase in thymic adiposity and is associated with reduced thymopoiesis independently of ghrelin signaling.
H. Yang, Y.-H. Youm, Y. Sun, J.-S. Rim, C. J. Galban, B. Vandanmagsar, and V. D. Dixit (2009)
J. Leukoc. Biol. 85, 928-938
   Abstract »    Full Text »    PDF »
Stem Cells Use Distinct Self-renewal Programs at Different Ages.
B.P. Levi and S.J. Morrison (2009)
Cold Spring Harb Symp Quant Biol
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Klotho ablation converts the biochemical and skeletal alterations in FGF23 (R176Q) transgenic mice to a Klotho-deficient phenotype.
X. Bai, Q. Dinghong, D. Miao, D. Goltzman, and A. C. Karaplis (2009)
Am J Physiol Endocrinol Metab 296, E79-E88
   Abstract »    Full Text »    PDF »
The Wnt modulator sFRP2 enhances mesenchymal stem cell engraftment, granulation tissue formation and myocardial repair.
M. P. Alfaro, M. Pagni, A. Vincent, J. Atkinson, M. F. Hill, J. Cates, J. M. Davidson, J. Rottman, E. Lee, and P. P. Young (2008)
PNAS 105, 18366-18371
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A genome-wide RNAi screen for Wnt/{beta}-catenin pathway components identifies unexpected roles for TCF transcription factors in cancer.
W. Tang, M. Dodge, D. Gundapaneni, C. Michnoff, M. Roth, and L. Lum (2008)
PNAS 105, 9697-9702
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Nuclear factor-{kappa}B-dependent reversal of aging-induced alterations in T cell cytokines.
M.-C. Huang, J.-J. Liao, S. Bonasera, D. L. Longo, and E. J. Goetzl (2008)
FASEB J 22, 2142-2150
   Abstract »    Full Text »    PDF »
Aging and Disease as Modifiers of Efficacy of Cell Therapy.
S. Dimmeler and A. Leri (2008)
Circ. Res. 102, 1319-1330
   Abstract »    Full Text »    PDF »
The impact of aging on kidney repair.
R. Schmitt and L. G. Cantley (2008)
Am J Physiol Renal Physiol 294, F1265-F1272
   Abstract »    Full Text »    PDF »
Gone with the Wnt/Notch: stem cells in laminopathies, progeria, and aging.
E. Meshorer and Y. Gruenbaum (2008)
J. Cell Biol. 181, 9-13
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Nuclear envelope defects cause stem cell dysfunction in premature-aging mice.
J. Espada, I. Varela, I. Flores, A. P. Ugalde, J. Cadinanos, A. M. Pendas, C. L. Stewart, K. Tryggvason, M. A. Blasco, J. M.P. Freije, et al. (2008)
J. Cell Biol. 181, 27-35
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Wnt makes stem cells act their age.
M. Leslie (2007)
J. Cell Biol. 178, 892
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Increased Wnt Signaling During Aging Alters Muscle Stem Cell Fate and Increases Fibrosis.
A. S. Brack, M. J. Conboy, S. Roy, M. Lee, C. J. Kuo, C. Keller, and T. A. Rando (2007)
Science 317, 807-810
   Abstract »    Full Text »    PDF »

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