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Fig. 3. mGluR5 agonists trigger astrocytic Ca2+ signaling in slices prepared from mice pups. (A) The left panel shows enhanced green fluorescent protein (eGFP) driven by the GLT1 promoter; the three right panels depict relative increase in rhod-2 fluorescence signal (F/F) in response to microinjection of t-ACPD in a hippocampal slice prepared from a 15-day-old mouse pup. The injection pipette is outlined. Green circle represents Alexa 488 wavefront (not shown). White arrows indicate astrocytes displaying an increase in Ca2+. (B) aCSF injection in a hippocampal slice. (Top) Changes in rhod-2 fluorescence emission (F/F); (bottom) Alexa 488 diffusion. (C to E) Histograms comparing (C) number of responding astrocytes, (D) Ca2+ amplitude, and (E) Ca2+ wave velocity and Alexa 488 diffusion velocity (internal control for consistency of agonist injection). ***P < 0.001, one-way ANOVA, Bonferroni test; n = 8 to 9 trials.