Nedd4 Controls Animal Growth by Regulating IGF-1 Signaling

Xiao R. Cao^1*, Nancy L. Lill^1*†, Natasha Boase², Peijun P. Shi¹, David R. Croucher³, Hongbo Shan¹, Jing Qu¹, Eileen M. Sweezer¹, Trenton Place¹, Patricia A. Kirby¹, Roger J. Daly³, Sharad Kumar^2,4†, and Baoli Yang^1‡

¹Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

²Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia.

³Garvan Institute of Medical Research, Sydney, NSW 2010, Australia.

⁴Department of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.

* These authors contributed equally to this work.

^†Present address: Department of Pathology, Ohio State University, 1645 Neil Avenue, Columbus, OH 43210, USA.

This PDF file includes:

• Supplementary Materials and Methods
• Fig. S1. MMRRC Nedd4 KO animals are growth retarded.
• Fig. S2. Some Nedd4^-/- embryos have ventricular septal defect (VSD).
• Fig. S3. Normal lung development in Nedd4^-/-embryos.
• Fig. S4. Mouse embryonic fibroblasts isolated from both Nedd4^+/- and Nedd4^-/- embryos show growth arrest at G₀–G₁ phase of the cell cycle.
• Fig. S5. Total cellular levels of the IGF-1R precursor and the processed β subunit do not vary consistently with Nedd4 gene dosage.
• Fig. S6. Cellular PTEN, c-Cbl, and Cbl-b levels are not significantly different in Nedd4^-/- MEFs.
• Fig. S7. Reexpression of Nedd4 in Nedd4^-/- MEFs restores IGF-1R to the cell surface.
• Fig. S8. Similar levels of Grb10 transcript in Nedd4^+/+ and Nedd4^-/- MEFs.
• Fig. S9. Generation and genotyping of Grb10-deficient mice.
• Fig. S10. Paternally inherited Grb10 allele is expressed at low levels in muscle.
• Supplementary References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 6.79 MB

^‡To whom correspondence should be addressed. E-mail: baoli-yang{at}uiowa.edu and sharad.kumar{at}imvs.sa.gov.au

Citation: X. R. Cao, N. L. Lill, N. Boase, P. P. Shi, D. R. Croucher, H. Shan, J. Qu, E. M. Sweezer, T. Place, P. A. Kirby, R. J. Daly, S. Kumar, B. Yang, Nedd4 Controls Animal Growth by Regulating IGF-1 Sci. Signal. 1, ra5 (2008).

© 2008 American Association for the Advancement of Science