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Sci. Signal., 23 September 2008
[DOI: 10.1126/scisignal.1160940]

Nedd4 Controls Animal Growth by Regulating IGF-1 Signaling

Xiao R. Cao1*, Nancy L. Lill1*, Natasha Boase2, Peijun P. Shi1, David R. Croucher3, Hongbo Shan1, Jing Qu1, Eileen M. Sweezer1, Trenton Place1, Patricia A. Kirby1, Roger J. Daly3, Sharad Kumar2,4, and Baoli Yang1

1Carver College of Medicine, University of Iowa, Iowa City, IA 52242, USA.

2Hanson Institute, Institute of Medical and Veterinary Science, Adelaide, SA 5000, Australia.

3Garvan Institute of Medical Research, Sydney, NSW 2010, Australia.

4Department of Medicine, University of Adelaide, Adelaide, SA 5005, Australia.

* These authors contributed equally to this work.

Present address: Department of Pathology, Ohio State University, 1645 Neil Avenue, Columbus, OH 43210, USA.

This PDF file includes:

  • Supplementary Materials and Methods
  • Fig. S1. MMRRC Nedd4 KO animals are growth retarded.
  • Fig. S2. Some Nedd4-/- embryos have ventricular septal defect (VSD).
  • Fig. S3. Normal lung development in Nedd4-/-embryos.
  • Fig. S4. Mouse embryonic fibroblasts isolated from both Nedd4+/- and Nedd4-/- embryos show growth arrest at G0G1 phase of the cell cycle.
  • Fig. S5. Total cellular levels of the IGF-1R precursor and the processed β subunit do not vary consistently with Nedd4 gene dosage.
  • Fig. S6. Cellular PTEN, c-Cbl, and Cbl-b levels are not significantly different in Nedd4-/- MEFs.
  • Fig. S7. Reexpression of Nedd4 in Nedd4-/- MEFs restores IGF-1R to the cell surface.
  • Fig. S8. Similar levels of Grb10 transcript in Nedd4+/+ and Nedd4-/- MEFs.
  • Fig. S9. Generation and genotyping of Grb10-deficient mice.
  • Fig. S10. Paternally inherited Grb10 allele is expressed at low levels in muscle.
  • Supplementary References

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Format: Adobe Acrobat PDF

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To whom correspondence should be addressed. E-mail: baoli-yang{at}uiowa.edu and sharad.kumar{at}imvs.sa.gov.au

Citation: X. R. Cao, N. L. Lill, N. Boase, P. P. Shi, D. R. Croucher, H. Shan, J. Qu, E. M. Sweezer, T. Place, P. A. Kirby, R. J. Daly, S. Kumar, B. Yang, Nedd4 Controls Animal Growth by Regulating IGF-1 Sci. Signal. 1, ra5 (2008).

© 2008 American Association for the Advancement of Science


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