Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 27 January 2009
[DOI: 10.1126/scisignal.2000014]

EGFR Signals to mTOR Through PKC and Independently of Akt in Glioma

Qi-Wen Fan,1,2,3,4 Christine Cheng,1,2,3,4 Zachary A. Knight,5,6,7 Daphne Haas-Kogan,3,4 David Stokoe,3,4 C. David James,3,4 Frank McCormick,4 Kevan M. Shokat,5,6,7 William A. Weiss1,2,3,4,5*

This PDF file includes:

  • Fig. S1. Inhibition or knockdown of EGFR, Akt, PI3K, and mTOR in glioma; effects of serum.
  • Fig. S2. PMA interferes with the ability of erlotinib to decrease phosphorylation of rpS6 in PTENwt glioma cells regardless of EGFR status.
  • Fig. S3. Inhibition of PTEN leads to increased abundance of p-Akt in PTENwt LN229: EGFR cells.
  • Fig. S4. Knockdown of PKCα and PKCδ in LN229:EGFR glioma cells.
  • Fig. S5. Abundance of EGFR, p-PKCα, p-PKC (pan), p-rpS6, and p-Akt in normal brain and primary human glioblastoma tumors.
  • Fig. S6. A PKC inhibitor blocks proliferation in both PTENwt and PTENmt glioma cell lines.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1,289 KB


1Department of Neurology, University of California, 533 Parnassus Avenue, San Francisco, CA 94143, USA. 2Department of Pediatrics, University of California, San Francisco, CA 94143, USA. 3Department of Neurological Surgery and Brain Tumor Research Center, University of California, San Francisco, CA 94143, USA. 4Comprehensive Cancer Center, University of California, San Francisco, CA 94143, USA. 5Program in Chemistry and Chemical Biology, University of California, San Francisco, CA 94158, USA. 6Department of Cellular and Molecular Pharmacology, University of California, San Francisco, CA 94158, USA. 7Howard Hughes Medical Institute, University of California, San Francisco, CA 94158, USA.

*To whom correspondence should be addressed. E-mail, weiss{at}cgl.ucsf.edu

Citation: Q.-W. Fan, C. Cheng, Z. A. Knight, D. Haas-Kogan, D. Stokoe, C. D. James, F. McCormick, K. M. Shokat, W. A. Weiss, EGFR Signals to mTOR Through PKC and Independently of Akt in Glioma. Sci. Signal. 2, ra4 (2009).

© 2009 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882