Brx Mediates the Response of Lymphocytes to Osmotic Stress Through
the Activation of NFAT5
Tomoshige Kino,1* Hiroaki Takatori,2 Irini Manoli,3,4
Yonghong Wang,5 Anatoly Tiulpakov,1 Marc R. Blackman,3,6 Yan A. Su,7
George P. Chrousos,1,4 Alan H. DeCherney,1 James H. Segars1
1Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver
National Institute of Child Health and Human Development, National Institutes
of Health, Bethesda, MD 20892, USA.
2Molecular Immunology and Inflammation
Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases,
National Institutes of Health, Bethesda, MD 20892, USA.
3Endocrine
Section, Laboratory of Clinical Investigation, National Center for Complementary
and Alternative Medicine, National Institutes of Health, Bethesda, MD
20892, USA.
4First Department of Pediatrics, Athens University, Athens 11527,
Greece.
5Clinical Molecular Profiling Core, Advanced Technology Center, National
Cancer Institute, National Institutes of Health, Gaithersburg, MD 20892,
USA.
6Washington DC VA Medical Center, Washington, DC 20422, USA.
7Department
of Biochemistry and Molecular Biology, the Catherine Birch McCormick
Genomics Center, George Washington University School of Medicine and Health
Sciences, Washington, DC 20037, USA.
This PDF file includes:
- Fig. S1. Brx-specific siRNAs suppress the expression of brx mRNA and attenuate
osmotic stimulus–induced expression of nfat5 mRNA in Jurkat cells.
- Table S1. Primer pairs used in real-time RT-PCR assays.
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*To whom correspondence should be addressed. E-mail: kinot{at}mail.nih.gov
Citation:
T. Kino, H. Takatori, I. Manoli, Y. Wang, A. Tiulpakov, M. R. Blackman, Y. A. Su,
G. P. Chrousos, A. H. DeCherney, J. H. Segars, Brx mediates the response of lymphocytes
to osmotic stress through the activation of NFAT5. Sci. Signal. 2, ra5 (2009).
© 2009 American Association for the Advancement of Science
