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Sci. Signal., 10 February 2009
[DOI: 10.1126/scisignal.2000081]

Brx Mediates the Response of Lymphocytes to Osmotic Stress Through the Activation of NFAT5

Tomoshige Kino,1* Hiroaki Takatori,2 Irini Manoli,3,4 Yonghong Wang,5 Anatoly Tiulpakov,1 Marc R. Blackman,3,6 Yan A. Su,7 George P. Chrousos,1,4 Alan H. DeCherney,1 James H. Segars1

1Program in Reproductive and Adult Endocrinology, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
2Molecular Immunology and Inflammation Branch, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
3Endocrine Section, Laboratory of Clinical Investigation, National Center for Complementary and Alternative Medicine, National Institutes of Health, Bethesda, MD 20892, USA.
4First Department of Pediatrics, Athens University, Athens 11527, Greece.
5Clinical Molecular Profiling Core, Advanced Technology Center, National Cancer Institute, National Institutes of Health, Gaithersburg, MD 20892, USA.
6Washington DC VA Medical Center, Washington, DC 20422, USA.
7Department of Biochemistry and Molecular Biology, the Catherine Birch McCormick Genomics Center, George Washington University School of Medicine and Health Sciences, Washington, DC 20037, USA.

This PDF file includes:

  • Fig. S1. Brx-specific siRNAs suppress the expression of brx mRNA and attenuate osmotic stimulus–induced expression of nfat5 mRNA in Jurkat cells.
  • Table S1. Primer pairs used in real-time RT-PCR assays.

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*To whom correspondence should be addressed. E-mail: kinot{at}mail.nih.gov

Citation: T. Kino, H. Takatori, I. Manoli, Y. Wang, A. Tiulpakov, M. R. Blackman, Y. A. Su, G. P. Chrousos, A. H. DeCherney, J. H. Segars, Brx mediates the response of lymphocytes to osmotic stress through the activation of NFAT5. Sci. Signal. 2, ra5 (2009).

© 2009 American Association for the Advancement of Science


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