SIK1 Couples LKB1 to p53-Dependent Anoikis and Suppresses Metastasis

Hailing Cheng, Pixu Liu, Zhigang C. Wang, Lihua Zou, Stephanie Santiago, Victoria Garbitt, Ole V. Gjoerup, J. Dirk Iglehart, Alexander Miron, Andrea L. Richardson, William C. Hahn,* Jean J. Zhao*

*To whom correspondence should be addressed. E-mail: jean_zhao{at}dfci.harvard.edu (J.J.Z) and william_hahn{at}dfci.harvard.edu (W.C.H.)

This PDF file includes:

• Fig. S1. Validation of SIK1 knockdown by shSIK1s.
• Fig. S2. SIK1 knockdown does not alter p53 mRNA abundance.
• Fig. S3. Loss of SIK1 results in AI colony growth.
• Fig. S4. Low SIK1 expression correlates with high malignant potential in ovarian cancer.
• Table S1. Kinase hits from a kinome-wide shRNA screen.
• Table S2. Experimental metastasis assay.

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Citation: H. Cheng, P. Liu, Z. C. Wang, L. Zou, S. Santiago, V. Garbitt, O. V. Gjoerup, J. D. Iglehart, A. Miron, A. L. Richardson, W. C. Hahn, J. J. Zhao, SIK1 couples LKB1 to p53-dependent anoikis and suppresses metastasis. Sci. Signal. 2, ra35 (2009).

© 2009 American Association for the Advancement of Science