The Akt1-eNOS Axis Illustrates the Specificity of Kinase-Substrate Relationships in Vivo

Michael Schleicher, Jun Yu, Takahisa Murata, Berhad Derakhshan, Dimitriy Atochin, Li Qian, Satoshi Kashiwagi, Annarita Di Lorenzo, Kenneth D. Harrison, Paul L. Huang,* William C. Sessa*

*To whom correspondence should be addressed. E-mail: huangp{at}helix.mgh.harvard.edu (P.L.H.) and william.sessa{at}yale.edu (W.C.S.)

This PDF file includes:

• Fig. S1. Generation of S1176D/Akt1^–/– and S1176A/Akt1^–/– mice.
• Fig. S2. NO production in mouse lung endothelial cells.
• Fig. S3. eNOS phosphorylation in tissue after hind-limb ischemia.
• Fig. S4. PGC-1α–dependent pathways are not involved in ischemic recovery.
• Fig. S5. NO-dependent stabilization of the HIF cascade requires PHD activity.

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Citation: M. Schleicher, J. Yu, T. Murata, B. Derakhshan, D. Atochin, L. Qian, S. Kashiwagi, A. Di Lorenzo, K. D. Harrison, P. L. Huang, W. C. Sessa, The Akt1-eNOS axis illustrates the specificity of kinase-substrate relationships in vivo. Sci. Signal. 2, ra41 (2009).

© 2009 American Association for the Advancement of Science