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Sci. Signal., 25 August 2009
[DOI: 10.1126/scisignal.2000274]

Supplementary Materials for:

The VDAC2-BAK Rheostat Controls Thymocyte Survival

Decheng Ren, Hyungjin Kim, Ho-Chou Tu, Todd D. Westergard, Jill K. Fisher, Jeff A. Rubens, Stanley J. Korsmeyer, James J.-D. Hsieh, Emily H.-Y. Cheng*

*To whom correspondence should be addressed. E-mail: echeng{at}dom.wustl.edu

This PDF file includes:

  • Fig. S1. VDAC1 and VDAC2 are found in a wide range of mouse tissues.
  • Fig. S2. Abundance of VDAC2 by genotype.
  • Fig. S3. Analysis of thymocyte numbers by genotype.
  • Fig. S4. Effect of the deficiency in Vdac2 on the abundance of other VDAC isoforms and of pro- and antiapoptotic proteins.
  • Fig. S5. Conditional deletion of Vdac2 results in enhanced apoptosis in DP thymocytes.
  • Fig. S6. WT and Vdac2 null thymocytes display comparable kinetics of BIM induction.
  • Fig. S7. Deficiency in Bak rescues the apoptotic phenotype associated with deletion of Vdac2.
  • Fig. S8. Deficiency in Bax fails to rescue the apoptotic phenotype associated with deletion of Vdac2.
  • Fig. S9. Deficiency in both Vdac2 and Bak does not affect homeostasis or survival of thymocytes.
  • Fig. S10. Vdac2 null thymocytes display an enhanced apoptotic response to TCR stimulation, which is rescued by deficiency in Bak, but not Bax.
  • Fig. S11. PCR analysis of Vdac2 deletion in thymocyte subsets.

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Citation: D. Ren, H. Kim, H.-C. Tu, T. D. Westergard, J. K. Fisher, J. A. Rubens, S. J. Korsmeyer, J. J.-D. Hsieh, E. H.-Y. Cheng, The VDAC2-BAK rheostat controls thymocyte survival. Sci. Signal. 2, ra48 (2009).

© 2009 American Association for the Advancement of Science


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