IL-1 Induces IGF-Dependent Epithelial Proliferation in Prostate Development and Reactive Hyperplasia

Travis J. Jerde* and Wade Bushman*

*To whom correspondence should be addressed. E-mail: jerde{at}urology.wisc.edu (T.J.J.) and bushman{at}urology.wisc.edu (W.B.)

This PDF file includes:

• Table S1. Relative expression of inflammatory mediators in the developing, adult, and inflamed prostate.
• Fig. S1. RT-PCR analysis of mRNA of IL-1 family members during prostate development.
• Fig. S2. Instillation of E. coli into mouse prostates incites inflammatory reactive hyperplasia.
• Fig. S3. Sectional in situ hybridization for IL-1α and IL-1β.
• Fig. S4. Quantitative analysis of epithelial outgrowth induced by exogenous IL-1 family members in ex vivo cultured urogenital sinus tissues in the absence of androgen.
• Fig. S5. Androgen production is unaltered in IL-1R1^(–/–) mice.
• Fig. S6. IL-1 induces epithelial progenitor cell expansion.
• Fig. S7. IL-1 receptor is activated in the prostate stroma during development.
• Fig. S8. Quantitative analysis of epithelial or stromal outgrowth induced by IL-1α (10 ng/ml) or IGF-1 in ex vivo cultured urogenital sinus tissues in the absence of androgen.

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Citation: T. J. Jerde, W. Bushman, IL-1 induces IGF-dependent epithelial proliferation in prostate development and reactive hyperplasia. Sci. Signal. 2, ra49 (2009).

© 2009 American Association for the Advancement of Science