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Sci. Signal., 8 September 2009
[DOI: 10.1126/scisignal.2000396]

Supplementary Materials for:

Proteomic Analysis of Integrin-Associated Complexes Identifies RCC2 as a Dual Regulator of Rac1 and Arf6

Jonathan D. Humphries, Adam Byron, Mark D. Bass, Sue E. Craig, John W. Pinney, David Knight, Martin J. Humphries*

*To whom correspondence should be addressed. E-mail: martin.humphries{at}

This PDF file includes:

  • Methods
  • Fig. S1. Disruption of the actin cytoskeleton does not affect recruitment of talin and paxillin to isolated α5β1–FN complexes.
  • Fig. S2. Knockdown of RCC2 accelerates adhesion formation.
  • Fig. S3. Knockdown of RCC2 augments FN-mediated cell spreading.
  • Table S1. Hierarchical clustering analysis of proteins identified in FN, VCAM-1, and VCAM-1(D40A) affinity purifications (full data sets).
  • Table S2. Specifically enriched proteins identified in FN and VCAM-1 affinity purifications.
  • Table S3. Protein-protein interaction network statistics.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 12.2 MB

Other Supplementary Materials for this manuscript include the following:

  • File 1. Table S1 (Microsoft Excel format)
  • File 2. Table S2 (Microsoft Excel format)
  • File 3. Interaction network model for the α5β1–FN complex (XGMML format).
  • File 4. Interaction network model for the α4β1–VCAM-1 complex (XGMML format).

[Download Tables (Compressed)]

Technical Details

Format: Microsoft Excel and XGMML formats [compressed]

The XGMML format is used to display graphs and networks and can be viewed by the Cytoscape program, which can be freely downloaded from

Size: 246 KB

Citation: J. D. Humphries, A. Byron, M. D. Bass, S. E. Craig, J. W. Pinney, D. Knight, M. J. Humphries, Proteomic analysis of integrin-associated complexes identifies RCC2 as a dual regulator of Rac1 and Arf6. Sci. Signal. 2, ra51 (2009).

© 2009 American Association for the Advancement of Science

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