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Sci. Signal., 16 February 2010
[DOI: 10.1126/scisignal.2000482]

Supplementary Materials for:

Deciphering Protein Kinase Specificity Through Large-Scale Analysis of Yeast Phosphorylation Site Motifs

Janine Mok, Philip M. Kim, Hugo Y. K. Lam, Stacy Piccirillo, Xiuqiong Zhou, Grace R. Jeschke, Douglas L. Sheridan, Sirlester A. Parker, Ved Desai, Miri Jwa, Elisabetta Cameroni, Hengyao Niu, Matthew Good, Attila Remenyi, Jia-Lin Nianhan Ma, Yi-Jun Sheu, Holly E. Sassi, Richelle Sopko, Clarence S. M. Chan, Claudio De Virgilio, Nancy M. Hollingsworth, Wendell A. Lim, David F. Stern, Bruce Stillman, Brenda J. Andrews, Mark B. Gerstein, Michael Snyder,* Benjamin E. Turk*

*To whom correspondence should be addressed. E-mail: ben.turk{at}yale.edu; mpsnyder{at}stanford.edu.

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Assay reproducibility.
  • Fig. S2. Representative peptide array screening results for the Kss1 S147E mutant.
  • Fig. S3. Representative Prk1 in vitro assays.
  • Table S1. Representative peptide array screening results and sequence logos for each of the 61 kinases assayed (also available as a separate PDF with high-resolution images below).
  • Table S2. Protein kinases analyzed in this study.
  • Table S3. Pairwise correlation coefficients for each of four genomic features and the Scansite match score.
  • Table S4. Alignment of yeast kinases analyzed in this study.
  • References

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Other Supplementary Material for this manuscript includes the following:

  • Table S1
[Download Supplementary Table S1 PDF (Compressed)]

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  • Data set S1. Average PWMs for each of the 61 kinases assayed (plain text files).
  • Data set S2. MOTIPS output for each of the 61 kinases assayed (plain text files).
  • Data set S3. MUSCLE alignment of all predicted S. cerevisiae ORFs with orthologs from 12 other yeast species (clustal alignment files).
  • Data set S4. Alignment of yeast kinases analyzed in this study (clustal alignment file).
[Download Data Set S1 (Compressed)]

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[Download Data Set S2 (Compressed)]

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Size: 6.8 MB (compressed); 17.8 MB (decompressed)

[Download Data Set 3 (Compressed)]

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Format: Clustal Alignment Files

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[Download Data Set 4 (Compressed)]

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Format: Clustal Alignment File

Size: 13 KB (compressed); 52 KB (decompressed)

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Citation: J. Mok, P. M. Kim, H. Y. K. Lam, S. Piccirillo, X. Zhou, G. R. Jeschke, D. L. Sheridan, S. A. Parker, V. Desai, M. Jwa, E. Cameroni, H. Niu, M. Good, A. Remenyi, J.-L. N. Ma, Y.-J. Sheu, H. E. Sassi, R. Sopko, C. S. M. Chan, C. De Virgilio, N. M. Hollingsworth, W. A. Lim, D. F. Stern, B. Stillman, B. J. Andrews, M. B. Gerstein, M. Snyder, B. E. Turk, Deciphering Protein Kinase Specificity Through Large-Scale Analysis of Yeast Phosphorylation Site Motifs. Sci. Signal. 3, ra12 (2010).

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