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Sci. Signal., 16 March 2010
[DOI: 10.1126/scisignal.2000651]

Supplementary Materials for:

A Loss-of-Function Screen Reveals Ras- and Raf-Independent MEK-ERK Signaling During Chlamydia trachomatis Infection

Rajendra Kumar Gurumurthy, André P. Mäurer, Nikolaus Machuy, Simone Hess, Klaus P. Pleissner, Johannes Schuchhardt, Thomas Rudel, Thomas F. Meyer*

*To whom correspondence should be addressed. E-mail: meyer{at}

This PDF file includes:

  • Fig. S1. Overview of the image analysis with Scan R analysis software.
  • Fig. S2. siRNA controls and plate layout.
  • Fig. S3. Correlation plot for platewise quality control.
  • Fig. S4. Two-step normalization for POC analysis.
  • Fig. S5. Images for validated hits.
  • Fig. S6. Infectivity phenotypes upon knockdown of Ras and Raf family members.
  • Descriptions of supplementary tables.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.7 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1. Primary screen data list (Excel file).
  • Table S2. Hit validation data list (Excel file).
  • Table S3. Final hit list (Excel file).
  • Table S4. Sequences of siRNAs used in the primary screen (Excel file).
  • Table S5. Sequences of the siRNAs used to validate the hits (Excel file).

.[Download Tables S1 to S5 (Compressed)]

Technical Details

Format: Microsoft Excel

Size: 1.1 MB (compressed); 2.8 MB (decompressed)

For files packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the following instructions.

Instructions for downloading and decompressing files:

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Citation: R. K. Gurumurthy, A. P. Mäurer, N. Machuy, S. Hess, K. P. Pleissner, J. Schuchhardt, T. Rudel, T. F. Meyer, A Loss-of-Function Screen Reveals Ras- and Raf-Independent MEK-ERK Signaling During Chlamydia trachomatis Infection. Sci. Signal. 3, ra21 (2010).

© 2010 American Association for the Advancement of Science

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