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Sci. Signal., 11 May 2010
[DOI: 10.1126/scisignal.2000645]

Supplementary Materials for:

Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse

Marco A. Purbhoo, Hebin Liu, Stephane Oddos, Dylan M. Owen, Mark A. A. Neil, Sophie V. Pageon, Paul M. W. French, Christopher E. Rudd, Daniel M. Davis*

*To whom correspondence should be addressed. E-mail: d.davis{at}imperial.ac.uk

This PDF file includes:

  • Fig. S1. DiI-stained intracellular vesicles are positioned proximal to the IS.
  • Fig. S2. The intracellular distribution of LAT.
  • Fig. S3. Clusters of SLP-76 align with the microtubule cytoskeleton.
  • Fig. S4. Analysis of the speed of protein cluster at the interface between Jurkat cells and OKT-3–coated coverslips.
  • Fig. S5. LAT is phosphorylated at the sites of contact between SLP-76 microclusters and LAT-containing vesicles.
  • Descriptions of movies S1 to S7.

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Technical Details

Format: Adobe Acrobat PDF

Size: 952 KB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. Dynamics of SLP-76 and GADS clusters (.avi format).
    [Download Movie S1]
  • Movie S2. Dynamics of SLP-76 and LAT clusters (.avi format).
    [Download Movie S2]
  • Movie S3. Dynamics of a single LAT cluster (.avi format).
    [Download Movie S3]
  • Movie S4. Dynamics of SLP-76, LAT, and intracellular vesicles (.avi format).
    [Download Movie S4]
  • Movie S5. Dynamics of SLP-76 clusters imaged by TIRF (.avi format).
    [Download Movie S5]
  • Movie S6. Dynamics of subsynaptic vesicles compared to those of clusters of ZAP-70 (.avi format).
    [Download Movie S6]
  • Movie S7. Dynamics of vesicular LAT in relation to clusters of ZAP-70 (.avi format).
    [Download Movie S7]

Technical Details

Format: AVI

Size: 2–9 MB (Varies by movie file)

Citation: M. A. Purbhoo, H. Liu, S. Oddos, D. M. Owen, M. A. A. Neil, S. V. Pageon, P. M. W. French, C. E. Rudd, D. M. Davis, Dynamics of Subsynaptic Vesicles and Surface Microclusters at the Immunological Synapse. Sci. Signal. 3, ra36 (2010).

© 2010 American Association for the Advancement of Science

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