TREM2- and DAP12-Dependent Activation of PI3K Requires DAP10 and Is Inhibited by SHIP1

Qisheng Peng, Shikha Malhotra, James A. Torchia, William G. Kerr, K. Mark Coggeshall, Mary Beth Humphrey*

*To whom correspondence should be addressed. E-mail: marybeth-humphrey{at}ouhsc.edu

This PDF file includes:

• Fig. S1. Recruitment of PLC-γ2 and Grb2 to DAP12 occurs upon ligation of TREM2.
• Fig. S2. DAP10 and DAP12 form homodimers and heterodimers in macrophages.
• Fig. S3. DAP10 is not required for the expression of TREM2 at the cell surface or for TREM2-induced Ca²⁺ flux.
• Fig. S4. Ligation of TREM2 leads to protection from apoptosis by inducing the
  production of MCL-1.
• Fig. S5. Inhibition of PI3K, but not of Src or Syk, increases the amount of SHIP1 recruited to DAP12.
• Fig. S6. SHIP1 and DAP10 colocalize with actin in cap-like structures after ligation
  of TREM2.
• Fig. S7. DAP10 is not required for the translocation of SHIP1 to the plasma
  membrane.

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