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Sci. Signal., 15 June 2010
[DOI: 10.1126/scisignal.2000681]

Supplementary Materials for:

Ablation of the Kinase NDR1 Predisposes Mice to the Development of T Cell Lymphoma

Hauke Cornils,* Mario R. Stegert, Alexander Hergovich, Debby Hynx, Debora Schmitz, Stephan Dirnhofer, Brian A. Hemmings*

*To whom correspondence should be addressed. E-mail: hauke.cornils{at}fmi.ch (H.C.); brian.hemmings{at}fmi.ch (B.A.H.)

This PDF file includes:

  • Fig. S1. Generation of NDR1-deficient mice.
  • Fig. S2. Mature T cells from NDR1+/– and NDR1–/– mice show slightly increased
    resistance to apoptosis.
  • Fig. S3. Activation of NDR is unchanged in NDR1-deficient thymocytes in response
    to induction of apoptosis by dexamethasone or antibody against Fas together with
    cycloheximide.
  • Fig. S4. NDR1 and NDR2 show distinct patterns in mice.
  • Fig. S5. The expression of NDR2 and the abundance of NDR2 protein remain
    unchanged upon loss of NDR1.
  • Fig. S6. Reduction of the abundance of NDR2 in NDR1-deficient MEFs results in
    increased resistance to apoptosis.
  • Fig. S7. Increased development of MPDs in NDR1+/– and NDR1–/– mice after ENU
    treatment.
  • Fig. S8. An increase in the abundance of Pou2af1 correlates with a low abundance of
    NDR in tumors.
  • Fig. S9. Loss of NDR1 results in minor defects in the proliferation of mature T cells
    after stimulation of the TCR.
  • Fig. S10. Cells from NDR-high and NDR-low tumors show no consistent differences
    in Ki67 staining.
  • Fig. S11. siRNA against MEF2c does not rescue apoptosis defects in cells depleted
    of NDR1 and NDR2.
  • Fig. S12. Characterization of a murine antibody against NDR1.
  • Fig. S13. Validation of siRNA against MEF2c and overexpression of E47 in
    transfected cells.
  • Fig. S14. Testing of different shNDR2 constructs.
  • Table S1. Aged mice analyzed for tumor development.
  • Table S2. Overview of tumors identified in ENU-treated mice.
  • Table S3. Classification of human T cell lymphoma samples.
  • Table S4. Classification of tumors according to the abundances of NDR1 and NDR2.
  • Table S5. Genes whose expression was significantly altered in NDR-low tumors.

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Citation: H. Cornils, M. R. Stegert, A. Hergovich, D. Hynx, D. Schmitz, S. Dirnhofer, B. A. Hemmings, Ablation of the Kinase NDR1 Predisposes Mice to the Development of T Cell Lymphoma. Sci. Signal. 3, ra47 (2010).

© 2010 American Association for the Advancement of Science


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