Origins and Diversification of a Complex Signal Transduction System in Prokaryotes

Kristin Wuichet and Igor B. Zhulin*

*To whom correspondence should be addressed. E-mail: ijouline{at}utk.edu

This PDF file includes:

• Table S1. Chemotaxis and motility components across 450 genomes.
• Table S2. MAC systems.
• Table S3. HKIII systems.
• Table S4. Sensor domain diversity of two-component and chemotaxis systems.
• Table S5. Domain architecture query sensitivities.
• Fig. S1. Maximum likelihood trees built from CheA, CheB, and CheR multiple sequence alignments.
• Fig. S2. Chemotaxis System Classification Step 1: Identifying coevolving CheA (A), CheB (B), and CheR (R) sequences.
• Fig. S3. Chemotaxis System Classification Step 2: Defining classes using phylogenomic markers.
• Fig. S4. Maximum likelihood tree built from a concatenated CheA-CheB-CheR multiple sequence alignment of CheA-CheB-CheR proteins.
• Fig. S5. Chemotaxis System Classification Step 3: Assigning unclassified CheA (A), CheB (B), and CheR (R) sequences to chemotaxis system classes.
• Fig. S6. Chemotaxis System Classification Step 4: Assigning CheC (C), CheD (D), CheV (V), CheW (W), CheX (X), and CheZ (Z) sequences to chemotaxis classes.
• Fig. S7. Maximum likelihood trees built from individual CheC, CheD, CheV, CheW, and CheZ multiple sequence alignments.
• Fig. S8. Multiple sequence alignment of the putative MAC1 methylation region.
• Fig. S9. Multiple sequence alignment of the putative MAC2 methylation region.
• Fig. S10. Multiple sequence alignment of the HKIII phosphotransfer and HATPase regions.
• References

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Excel file, 234 KB)
• Table S2 (Excel file, 40 KB)

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