Autocrine Purinergic Receptor Signaling Is Essential for Macrophage
Chemotaxis

Moritz Kronlage, Jian Song, Lydia Sorokin, Katrin Isfort, Tanja Schwerdtle, Jens Leipziger, Bernard Robaye, Pamela B. Conley, Hee-Cheol Kim, Sarah Sargin, Peter Schön, Albrecht Schwab, Peter J. Hanley*

*To whom correspondence should be addressed. E-mail: hanley{at}uni-muenster.de

This PDF file includes:

• Fig. S1. Reversed-phase HPLC analysis of ATP degradation by potato apyrase.
• Fig. S2. Inhibition of the adenosine feedback loop with 8-SPT.
• Fig. S3. Chemotaxis is impaired by blocking ecto-ATPase in P2ry2-deficient
  macrophages.
• Fig. S4. Chemotaxis is not impaired in Panx1-deficient macrophages.
• Fig. S5. The P2Y₁₂ receptor antagonist AR-C69931MX blocks ADP-β-S–induced
  lamellipodial membrane protrusions.
• Descriptions of movies S1 to S13.

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Other Supplementary Material for this manuscript includes the following:

• Movie S1. Wild-type macrophages in a C5a chemotactic gradient (.mov format).
  [Download Movie S1]
• Movie S2. Wild-type macrophages in a C5a chemotactic gradient with apyrase (40 U/ml) (.mov format).
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• Movie S3. P2ry2^–/– macrophages in a C5a chemotactic gradient (.mov format).
  [Movie Movie S3]
• Movie S4. P2ry12^–/– macrophages in a C5a chemotactic gradient (.mov format).
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• Movie S5. P2ry2^–/– macrophages in a C5a chemotactic gradient with AR-C69931MX (10 μM) (.mov format).
  [Download Movie S5]
• Movie S6. P2ry2^–/– macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).
  [Download Movie S6]
• Movie S7. P2ry12^–/– macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).
  [Download Movie S7]
• Movie S8. Wild-type macrophages in a C5a chemotactic gradient with 8-SPT (100 μM) (.mov format).
  [Download Movie S8]
• Movie S9. P2ry2^–/– macrophages in a C5a chemotactic gradient with "cocktail block" (.mov format).
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• Movie S10. P2ry2^–/– macrophages in a C5a chemotactic gradient with "triple block" (.mov format).
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• Movie S11. Panx1^–/– macrophages in a C5a chemotactic gradient (.mov format).
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• Movie S12. ATP-induced lamellipodial membrane protrusions (.mov format).
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• Movie S13. Lack of ATP-induced lamellipodial formation in P2ry2^–/– macrophages (.mov format).
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