Decoupling of Receptor and Downstream Signals in the Akt Pathway by
Its Low-Pass Filter Characteristics

Kazuhiro A. Fujita, Yu Toyoshima, Shinsuke Uda, Yu-ichi Ozaki, Hiroyuki Kubota, Shinya Kuroda*

*To whom correspondence should be addressed. E-mail: skuroda{at}bi.s.u-tokyo.ac.jp

This PDF file includes:

• Fig. S1. Gel images of immunoblots.
• Fig. S2. Akt pathway model.
• Fig. S3. Dose responses of pEGFR, pAkt, and pS6.
• Fig. S4. EGF-dependent phosphorylation of S6K.
• Fig. S5. Inhibition of S6 phosphorylation by LY294002 or rapamycin.
• Fig. S6. Concept of frequency response analysis.
• Fig. S7. Frequency response analysis of the Akt pathway model.
• Fig. S8. Responses of the fitted consecutive reactions.
• Fig. S9. NGF-dependent Akt pathway.
• Fig. S10. NGF-dependent Akt pathway model.
• Fig. S11. Time courses of the low-, middle-, and high-frequency waves of pEGFR, pAkt, and pS6.
• Fig. S12. Inhibitor model.
• Fig. S13. Parameter sensitivity analysis of EGF-dependent Akt pathway model.

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Other Supplementary Material for this manuscript includes the following:

• Model files in COPASI (.cps) and SBML (.xml) format for the EGF-Akt pathway model, the NGF-Akt pathway model, and the inhibitor pathway model.

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