Self-Induced Patched Receptor Down-Regulation Modulates Cell Sensitivity to the Hedgehog Morphogen Gradient

Andreu Casali*

*To whom correspondence should be addressed. E-mail: actbmc{at}ibmb.csic.es

This PDF file includes:

• Fig. S1. In situ hybridization to detect GFP-positive transcripts.
• Fig. S2. Ptc^Δ2-GFP (from the L>Ptc^Δ2-GFP transgene) is down-regulated in leg and haltere discs.
• Fig. S3. Ptc^LDL is endocytosed and sequesters Hh, but does not repress Smo.
• Fig. S4. Overexpression of Dispatched (Disp) does not induce degradation of Ptc-GFP (from the L>Ptc-GFP transgene).
• Fig. S5. Ptc^AAAA-GFP regulates Smo activity in the absence of endogenous Ptc.
• Fig. S6. The GFP tag does not affect the ability of Ptc^Δ2 to interact with endogenous Ptc.

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