Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 24 August 2010
[DOI: 10.1126/scisignal.2001059]

Supplementary Materials for:

Self-Induced Patched Receptor Down-Regulation Modulates Cell Sensitivity to the Hedgehog Morphogen Gradient

Andreu Casali*

*To whom correspondence should be addressed. E-mail: actbmc{at}ibmb.csic.es

This PDF file includes:

  • Fig. S1. In situ hybridization to detect GFP-positive transcripts.
  • Fig. S2. PtcΔ2-GFP (from the L>PtcΔ2-GFP transgene) is down-regulated in leg and haltere discs.
  • Fig. S3. PtcLDL is endocytosed and sequesters Hh, but does not repress Smo.
  • Fig. S4. Overexpression of Dispatched (Disp) does not induce degradation of Ptc-GFP (from the L>Ptc-GFP transgene).
  • Fig. S5. PtcAAAA-GFP regulates Smo activity in the absence of endogenous Ptc.
  • Fig. S6. The GFP tag does not affect the ability of PtcΔ2 to interact with endogenous Ptc.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 952 KB


Citation: A. Casali, Self-Induced Patched Receptor Down-Regulation Modulates Cell Sensitivity to the Hedgehog Morphogen Gradient. Sci. Signal. 3, ra63 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882