Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.


Sci. Signal., 19 October 2010
[DOI: 10.1126/scisignal.2001275]

Supplementary Materials - Patterson et al.
Supplementary Materials for:

Single-Cell Analysis Reveals That Insulation Maintains Signaling Specificity Between Two Yeast MAPK Pathways with Common Components

Jesse C. Patterson, Evguenia S. Klimenko, and Jeremy Thorner*

*To whom correspondence should be addressed. E-mail: jthorner{at}berkeley.edu

This PDF file includes:

  • Materials and Methods
  • Results
  • Fig. S1. Co-stimulation for 1 hour results in coactivation of the HOG and mating pathway transcriptional reporters in single cells.
  • Fig. S2. Co-stimulation for 2 hours results in further coactivation of the HOG and mating pathway transcriptional reporters in single cells.
  • Fig. S3. Flow cytometry confirms that dual stimulation leads to dual pathway activation in individual cells.
  • Fig. S4. Restricting MAPK activation to the Sln1 branch of the HOG pathway does not alter HOG and mating pathway reporter coexpression.
  • Fig. S5. Restricting MAPK activation to the Sho1 branch of the HOG pathway does not alter HOG and mating pathway reporter coexpression.
  • Fig. S6. Sonication followed by concurrent stimulation with high concentrations of sorbitol and α-factor causes cell death.
  • Fig. S7. Sequential stimulation of cells does not result in mutually exclusive, or any other history-dependent change in, HOG and mating pathway reporter expression.
  • Fig. S8. Restricting MAPK activation by the mating pathway to Fus3 does not alter HOG and mating pathway reporter coexpression.
  • Fig. S9. Restricting MAPK activation by the mating pathway to Kss1 does not alter HOG and mating pathway reporter coexpression.
  • Fig. S10. Both Kss1- and Ste5-dependent Fus3 activation contribute to cross talk in Hog1-deficient cells.
  • Fig. S11. Sustained Hog1 catalytic activity is required to prevent cross talk to the mating pathway.
  • Fig. S12. Validation of analog-sensitive KSS1 alleles.
  • Fig. S13. Lack of Hog1 phosphorylation of Ste50 or Pbs2 does not permit cross talk.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 7.8 MB


Citation: J. C. Patterson, E. S. Klimenko, J. Thorner, Single-Cell Analysis Reveals That Insulation Maintains Signaling Specificity Between Two Yeast MAPK Pathways with Common Components. Sci. Signal. 3, ra75 (2010).

© 2010 American Association for the Advancement of Science


To Advertise     Find Products


Science Signaling. ISSN 1937-9145 (online), 1945-0877 (print). Pre-2008: Science's STKE. ISSN 1525-8882