A Genome-Wide RNAi Screen Identifies Core Components of the G₂-M DNA Damage Checkpoint

Shu Kondo and Norbert Perrimon*

*To whom correspondence should be addressed. E-mail: perrimon{at}receptor.med.harvard.edu

This PDF file includes:

• Fig. S1. Caffeine-sensitive G₂-M checkpoint of Drosophila S2R+ cells.
• Fig. S2. Cell cycle profiles of cells depleted of the candidate genes.
• Fig. S3. Secondary screen for mitotic exit defects.
• Fig. S4. G₂-M checkpoint defect of cells depleted of the candidate genes.
• Fig. S5. DNA damage–induced phosphorylation of histone H2Av in cells depleted of candidate genes.
• Fig. S6. Mitotic index and cell cycle profile of cells depleted of pre-IC proteins.
• Table S1. Genes required for the G₂-M checkpoint induced by doxorubicin.
• Table S2. Primary hits excluded from further analysis and reasons for exclusion.
• Table S3. Statistics of the secondary screens.
• Table S4. Sequences of primers used for synthesizing dsRNA templates.
• References
  

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1,305 KB

© 2011 American Association for the Advancement of Science