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Sci. Signal., 11 January 2011
[DOI: 10.1126/scisignal.4155tr1]

Signaling by Neuronal Swelling (Movies)

R. Douglas Fields*

*E-mail, fieldsd{at}mail.nih.gov

Movie 1. High-magnification video of changes in light transmission in axons of electrically stimulated neurons. This time-lapse video shows axons from mouse dorsal root ganglion neurons grown in multicompartment cell cultures that are equipped with electrodes for electrical stimulation (Fig. 1). By subtracting a reference image before electrical stimulation and then performing image processing for the changes in intensity of light transmission after electrical stimulation, the increase in light transmission through axons (caused by axon swelling accompanying the transmembrane ion and water flux during membrane depolarization) can be seen. Increased light transmission is displayed as warmer colors. This is a 2-min time-lapse video showing the response to stimulation at 10 impulses per second for 60 s. Optical changes in axons such as this can be seen after firing a single impulse. Field width is 80 μm.

[Play Movie 1 (WMV)] [Play Movie 1 (MOV)]

Technical Details

Format: WMV; MOV

Size: 281 KB (WMV); 1,629 KB (MOV)

Requirements: Windows Media Player (WMV); QuickTime (MOV)

Movie 2. High-magnification video showing the microscopic displacement of an axon of a cultured neuron stimulated to fire electrical impulses. This time-lapse video shows a mouse dorsal root ganglion axon "twitching" after it was electrically stimulated. This is a 60-s time-lapse video after stimulating the axon at a frequency of 10 impulses per second for 5 s. This image appears fuzzy because the high magnification is near the limit of resolution of the light microscope and the pixel density of the video camera. The axon displacement in this experiment is approximately 0.5 μm. Field width is 30 μm.

[Play Movie 2 (WMV)] [Play Movie 2 (MOV)]

Technical Details

Format: WMV; MOV

Size: 166 KB (WMV); 178 KB (MOV)

Requirements: Windows Media Player (WMV); QuickTime (MOV)

Movie 3. Single-photon imaging of ATP released from cultured mouse dorsal root ganglion neurons. This time-lapse video shows one neuron (round cell in the middle of the video with the nucleus in the center) and several axons from many other neurons in the background. A high-gain image intensifier was used to detect single photons generated by the ATP-dependent reaction, which appear as red spots in the video. Notice the flash of photons when the neuron is given a weak electrical shock to make it fire electrical impulses. This is a time-lapse movie of a 10-min observation in response to 60 s stimulation of 10 impulses per second. Field with is 130 μm.

[Play Movie 3 (WMV)] [Play Movie 3 (MOV)]

Technical Details

Format: WMV; MOV

Size: 182 KB (WMV); 345 KB (MOV)

Requirements: Windows Media Player (WMV); QuickTime (MOV)

Limits for Use

Cost: Free

Rights: This material may be downloaded for noncommercial, course-teaching purposes only, provided credit to Science Signaling is included by listing the citation for the Teaching Resource.

Citation: R. D. Fields, Signaling by Neuronal Swelling. Sci. Signal. 4, tr1 (2011).

 


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