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Sci. Signal., 22 February 2011
[DOI: 10.1126/scisignal.2001731]

Supplementary Materials for:

ER Stress Inhibits mTORC2 and Akt Signaling Through GSK-3&beta–Mediated Phosphorylation of Rictor

Chien-Hung Chen, Tattym Shaikenov, Timothy R. Peterson, Rakhan Aimbetov,
Amangeldy K. Bissenbaev, Szu-Wei Lee, Juan Wu, Hui-Kuan Lin, Dos D. Sarbassov*

*To whom correspondence should be addressed. E-mail: dsarbass{at}mdanderson.org

This PDF file includes:

  • Fig. S1. Validation of the phospho-rictor (Ser1235) antibody and alignment of rictor orthologs.
  • Fig. S2. Effect of GSK-3 inhibition on the mTORC2 complex.
  • Fig. S3. GSK-3–dependent regulation of Akt is conserved in the invertebrate fly D. melanogaster.
  • Fig. S4. The rate of cell proliferation is dependent on phosphorylation of rictor at Ser1235.
  • Fig. S5. Analysis of rictor and Ras expression in MEFs.
  • Table S1. Quantification of the indicated immunoblotting signals.

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Technical Details

Format: Adobe Acrobat PDF

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Citation: C.-H. Chen, T. Shaikenov, T. R. Peterson, R. Aimbetov, A. K. Bissenbaev, S.-W. Lee, J. Wu, H.-K. Lin, D. D. Sarbassov, ER Stress Inhibits mTORC2 and Akt Signaling Through GSK-3&beta–Mediated Phosphorylation of Rictor. Sci. Signal. 4, ra10 (2011).

© 2011 American Association for the Advancement of Science


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