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Sci. Signal., 1 March 2011
[DOI: 10.1126/scisignal.2001270]

Supplementary Materials for:

ATP Inhibits the Generation and Function of Regulatory T Cells Through the Activation of Purinergic P2X Receptors

Ursula Schenk, Michela Frascoli, Michele Proietti, Robert Geffers, Elisabetta Traggiai, Jan Buer, Camillo Ricordi, Astrid M. Westendorf, Fabio Grassi*

*To whom correspondence should be addressed. E-mail: fabio.grassi{at}irb.unisi.ch

This PDF file includes:

  • Fig. S1. Increased suppressive function of p2rx7–/– Tregs.
  • Fig. S2. Correlation between extent of ERK phosphorylation and variations in intracellular ATP concentration.
  • Fig. S3. Impaired phosphorylation of ERK in p2rx7–/– Tregs.
  • Fig. S4. Increases in intracellular ATP concentration and Rorc mRNA abundance in Tregs correlate with TCR signal strength.
  • Fig. S5. P2X7-mediated expression of Il17a in Tregs.
  • Fig. S6. Conversion of Tconv cells to Tregs by P2X antagonism.
  • Fig. S7. Generation of Tregs by oATP and the additive effect of TGF-β.
  • Fig. S8. Effects of TGF-β and oATP on the expression of genes whose products interact within the TGF-β pathway.

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Citation: U. Schenk, M. Frascoli, M. Proietti, R. Geffers, E. Traggiai, J. Buer, C. Ricordi, A. M. Westendorf, F. Grassi, ATP Inhibits the Generation and Function of Regulatory T Cells Through the Activation of Purinergic P2X Receptors. Sci. Signal. 4, ra12 (2011).

© 2011 American Association for the Advancement of Science


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