In Vivo Systems Analysis Identifies Spatial and Temporal Aspects of the Modulation of TNF-α–Induced Apoptosis and Proliferation by MAPKs

Ken S. Lau, Alwin M. Juchheim, Kimberly R. Cavaliere, Sarah R. Philips, Douglas A. Lauffenburger, Kevin M. Haigis*

*To whom correspondence should be addressed. E-mail: khaigis{at}partners.org

This PDF file includes:

• Fig. S1. Basal proliferation and apoptosis in the small intestinal epithelium.
• Fig. S2. Effects of loss of TNFR1 or TNFR2 on TNF-α–induced signaling.
• Fig. S3. A representative TNF-α signaling network.
• Fig. S4. Time courses of protein phosphorylation signals activated after intravenous administration of TNF-α in vivo.
• Fig. S5. Total protein abundance and localization of activation in vivo.
• Fig. S6. Statistical modeling of the TNF-α signaling network in vivo.
• Fig. S7. Signaling data obtained after treatment with PD325901 or vehicle.
• Fig. S8. Statistical modeling of TNF-α–induced apoptosis in the context of MEK inhibition.
• Fig. S9. Statistical modeling of TNF-α–induced proliferation in the context of MEK inhibition.
  

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© 2011 American Association for the Advancement of Science