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Sci. Signal., 3 May 2011
[DOI: 10.1126/scisignal.2001791]

Supplementary Materials for:

Male Fertility Depends on Ca2+ Absorption by TRPV6 in Epididymal Epithelia

Petra Weissgerber, Ulrich Kriebs, Volodymyr Tsvilovskyy, Jenny Olausson, Oliver Kretz, Christof Stoerger, Rudi Vennekens, Ulrich Wissenbach, Ralf Middendorff, Veit Flockerzi, Marc Freichel*

*To whom correspondence should be addressed. E-mail: marc.freichel{at}

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Normal segregation of the Trpv6mt allele and weight gain in Trpv6mt/mt mice.
  • Fig. S2. Characterization of TRPV6 antibodies.
  • Fig. S3. Conversion of the Trpv6L2F2 allele into a Trpv6 null allele (Trpv6) by Cre-mediated recombination in mice.
  • Fig. S4. Ca2+ concentration and cAMP content in wild-type and Trpv6mt/mt sperm.
  • Fig. S5. Alizarin red staining in the cauda epididymis and histological analysis of prostates from wild-type and Trpv6mt/mt mice.
  • References

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.20 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi and .mov format). Capacitated sperm from the cauda epididymis of wild-type mice.
  • Movie S2 (.avi and .mov format). Capacitated sperm from the cauda epididymis of Trpv6mt/mt mice.

[Download Movies S1 and S2 (Compressed)]

Technical Details

Format: AVI and Quicktime Movie. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 30.9 MB (compressed); 77.4 MB (decompressed)

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
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Citation: P. Weissgerber, U. Kriebs, V. Tsvilovskyy, J. Olausson, O. Kretz, C. Stoerger, R. Vennekens, U. Wissenbach, R. Middendorff, V. Flockerzi, M. Freichel, Male Fertility Depends on Ca2+ Absorption by TRPV6 in Epididymal Epithelia. Sci. Signal. 4, ra27 (2011).

© 2011 American Association for the Advancement of Science

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