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Sci. Signal., 14 June 2011
[DOI: 10.1126/scisignal.2001750]

Supplementary Materials for:

Structure of a Pentavalent G-Actin•MRTF-A Complex Reveals How G-Actin Controls Nucleocytoplasmic Shuttling of a Transcriptional Coactivator

Stéphane Mouilleron, Carola A. Langer, Sebastian Guettler, Neil Q. McDonald,* Richard Treisman*

*To whom correspondence should be addressed. E-mail: Neil.McDonald{at} (N.Q.M.); Richard.Treisman{at} (R.T.)

This PDF file includes:

  • Fig. S1. CD spectrum for MRTF-A RPEL domain.
  • Fig. S2. Arrangement of actin molecules and structural subdomains in the pentavalent actin•RPEL assembly compared with those in the actin filament.
  • Fig. S3. Individual RPEL motifs and their extensions engage one or two actins within the pentameric complex.
  • Fig. S4. Comparison of pentavalent and trivalent actin•RPEL domain complexes.
  • Fig. S5. Actin S2 exhibits higher temperature factors than other actins in the pentavalent actin•RPEL domain assembly.
  • Fig. S6. TLS refinement shows higher mobility of actin S2 in the crystal lattice.
  • Fig. S7. Comparison of the interactions of RPELs and spacers close to the C terminus of actin in the pentameric actin•RPEL domain assembly.
  • Fig. S8. Size exclusion chromatography of G-actin•RPEL peptide and actin•RPEL domain complexes.
  • Fig. S9. Nuclear import properties of spacer mutants.
  • Fig. S10. Relationships of the actin•RPEL assemblies to import and export.
  • References
  • Legends for movies S1 and S2

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.13 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1. The pentavalent actin•RPEL domain assembly.
  • Movie S2. RPEL-actins R1, R2, and R3 align around the crank axis, in intimate contact with the RPEL domain.

[Download Movies S1 and S2 (Compressed)]

Technical Details

Format: Quicktime Movie. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

Size: 5.37 MB (compressed); 5.43 MB (decompressed)

Instructions for downloading and decompressing files:

  1. Create a temporary folder on your machine's hard drive.
  2. Save the compressed archive to the temporary folder you created, using the links above.
  3. Decompress the compressed file in the temporary folder using decompression software such as WinZip (Windows; or StuffIt Expander (Windows and Mac;

Citation: S. Mouilleron, C. A. Langer, S. Guettler, N. Q. McDonald, R. Treisman, Structure of a Pentavalent G-Actin•MRTF-A Complex Reveals How G-Actin Controls Nucleocytoplasmic Shuttling of a Transcriptional Coactivator. Sci. Signal. 4, ra40 (2011).

© 2011 American Association for the Advancement of Science

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