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Sci. Signal., 28 June 2011
[DOI: 10.1126/scisignal.2001796]

Supplementary Materials for:

Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling

Jes Alexander, Daniel Lim, Brian A. Joughin, Björn Hegemann, James R. A. Hutchins, Tobias Ehrenberger, Frank Ivins, Fabio Sessa, Otto Hudecz, Erich A. Nigg, Andrew M. Fry, Andrea Musacchio, P. Todd Stukenberg, Karl Mechtler, Jan-Michael Peters, Stephen J. Smerdon, Michael B. Yaffe*

*To whom correspondence should be addressed. E-mail: myaffe{at}mit.edu

This PDF file includes:

  • Fig. S1. Experimental approach for determining protein kinase motifs using positional scanning oriented peptide library screening (PS-OPLS).
  • Fig. S2. An experimental strategy for the identification of Plk1-dependent substrate sites.
  • Fig. S3. Conservation of specificity-determining residues in Polo-like kinase 1.
  • Fig. S4. Conservation of specificity-determining residues in Aurora B.
  • Fig. S5. Conservation of specificity-determining residues in Nek2.
  • Table S1. Characterization of Plk1-dependent phosphorylation sites.
  • Table S2. Statistical significance of co-occurrence of Scansite kinase substrate site predictions within proteins.
  • Details regarding data availability.

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Technical Details

Format: Adobe Acrobat PDF

Size: 3.8 MB


Citation: J. Alexander, D. Lim, B. A. Joughin, B. Hegemann, J. R. A. Hutchins, T. Ehrenberger, F. Ivins, F. Sessa, O. Hudecz, E. A. Nigg, A. M. Fry, A. Musacchio, P. T. Stukenberg, K. Mechtler, J.-M. Peters, S. J. Smerdon, M. B. Yaffe, Spatial Exclusivity Combined with Positive and Negative Selection of Phosphorylation Motifs Is the Basis for Context-Dependent Mitotic Signaling. Sci. Signal. 4, ra42 (2011).

© 2011 American Association for the Advancement of Science


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