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Sci. Signal., 28 June 2011
[DOI: 10.1126/scisignal.2001588]

Supplementary Materials for:

Mitotic Substrates of the Kinase Aurora with Roles in Chromatin Regulation Identified Through Quantitative Phosphoproteomics of Fission Yeast

André Koch, Karsten Krug, Stuart Pengelley, Boris Macek,* Silke Hauf*

*To whom correspondence should be addressed. E-mail: boris.macek{at}uni-tuebingen.de (B.M.); silke.hauf{at}tuebingen.mpg.de (S.H.)

This PDF file includes:

  • Fig. S1. Comparison of phosphorylated proteins identified in this and two other large-scale studies of fission yeast.
  • Fig. S2. Comparison of phosphopeptide ratios before and after normalization for protein abundance.
  • Fig. S3. Fragmentation mass spectra of the histone H3 peptide carrying the known Aurora target site (Ser10).
  • Fig. S4. Consensus motif analysis of phosphorylation sites that depend on the kinase activity of fission yeast Aurora.
  • Fig. S5. Consensus motif analysis of previously identified yeast Aurora or metazoan Aurora B phosphorylation sites.
  • Fig. S6. Reanalysis of the frequency of motif occurrence by including the K-X-[ST] and excluding the doubly phosphorylated sites.
  • Fig. S7. GO term enrichment of proteins with class 1 and 2 phosphorylation sites.
  • Fig. S8. Genetic interaction tests of ark1 temperature-sensitive mutants and previously identified genetic interactions of fission yeast Aurora substrates.
  • Table S1. Summary of MS data on all identified phosphorylation events.
  • Table S2. Number of phosphorylation sites within structured or unstructured regions.
  • Table S3. List of previously known yeast Aurora or metazoan Aurora B phosphorylation sites.
  • Table S4. List of GO terms and their associated proteins from class 1 and 2.
  • Table S5. List of proteins with class 1 or 2 sites tested for phosphorylation by fission yeast Aurora in vitro.
  • Table S6. Recombinant proteins or protein fragments used for the in vitro kinase assays.
  • Experimental Procedures
  • References

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. Summary of MS data on all identified phosphorylation events.

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Technical Details

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Citation: A. Koch, K. Krug, S. Pengelley, B. Macek, S. Hauf, Mitotic Substrates of the Kinase Aurora with Roles in Chromatin Regulation Identified Through Quantitative Phosphoproteomics of Fission Yeast. Sci. Signal. 4, rs6 (2011).

© 2011 American Association for the Advancement of Science


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