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Sci. Signal., 9 August 2011
[DOI: 10.1126/scisignal.2001681]

Supplementary Materials for:

A Polymorphism-Specific "Memory" Mechanism in the β2-Adrenergic Receptor

Andrea Ahles, Francesca Rochais, Torsten Frambach, Moritz Bünemann, Stefan Engelhardt*

*To whom correspondence should be addressed. E-mail: stefan.engelhardt{at}tum.de

This PDF file includes:

  • Fig. S1. Characterization of the β2AR FRET sensor.
  • Fig. S2. β2AR activation at half-maximal agonist concentrations, kinetics upon short-term stimulation, and assessment of internalization.
  • Fig. S3. β2AR desensitization.
  • Fig. S4. Phosphorylation of the polymorphic β2AR sensors and activation kinetics.
  • Table S1. Characteristics of ligand binding and β2AR sensor expression in intact cells, FRET responses for submaximal (Ki), and maximal agonist concentrations.

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Technical Details

Format: Adobe Acrobat PDF

Size: 6.22 MB

Citation: A. Ahles, F. Rochais, T. Frambach, M. Bünemann, S. Engelhardt, A Polymorphism-Specific "Memory" Mechanism in the β2-Adrenergic Receptor. Sci. Signal. 4, ra53 (2011).

© 2011 American Association for the Advancement of Science

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