Feedback Circuits Monitor and Adjust Basal Lck-Dependent Events in T Cell Receptor Signaling

Jamie R. Schoenborn, Ying Xim Tan, Chao Zhang, Kevan M. Shokat, Arthur Weiss*

*To whom correspondence should be addressed. E-mail: aweiss{at}medicine.ucsf.edu

This PDF file includes:

• Fig. S1. Screen of PP1 analogs for a specific inhibitor of Csk^AS.
• Fig. S2. Phosphorylation of ζ chain and its association with ZAP-70 upon inhibition of Csk^AS.
• Fig. S3. Inhibition of Csk^AS in primary mouse T cells activates Lck and ERK.
• Fig. S4. Csk^AS activity regulates the amount of the TCR at the cell surface.
• Fig. S5. Cellular activation induced by Csk^AS inhibition requires TCR at the cell surface, but is not dependent on the amount of TCR.
• Fig. S6. Increase in TCR abundance in response to Csk^AS is not required for cellular activation upon inhibition of Csk^AS.
• Fig. S7. Identification of Dok-1 as p65 by mass spectrometric analysis of Csk^AS immunoprecipitates.

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© 2011 American Association for the Advancement of Science