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Sci. Signal., 20 September 2011
[DOI: 10.1126/scisignal.2001619]

Supplementary Materials for:

Shigella flexneri Infection Generates the Lipid PI5P to Alter Endocytosis and Prevent Termination of EGFR Signaling

Damien Ramel, Frédéric Lagarrigue, Véronique Pons, Joëlle Mounier, Sophie Dupuis-Coronas, Gaëtan Chicanne, Philippe J. Sansonetti, Frédérique Gaits-Iacovoni, Hélène Tronchère, Bernard Payrastre*

*To whom correspondence should be addressed. E-mail: bernard.payrastre{at}inserm.fr

This PDF file includes:

  • Fig. S1. Exogenous short-chain PI5P induces activation of EGFR and protects EGFR from degradation.
  • Fig. S2. Quantification of immunofluorescence and Western blots corresponding to Fig. 1.
  • Fig. S3. Phosphoinositide concentrations in HeLa cells expressing EGFP, EGFP-IpgD-WT, or EGFP-Inp54p.
  • Fig. S4. Characterization of the Tet-Off IpgD-WT HeLa stable cell line.
  • Fig. S5. PI5P production activates EGFR in the absence of detectable ligand.
  • Fig. S6. Quantification of Western blots corresponding to Fig. 2F.
  • Fig. S7. Cholesterol depletion attenuates IpgD-induced Akt activation.
  • Fig. S8. Quantification of Western blots corresponding to Fig. 3A.
  • Fig. S9. Transformation of PI(4,5)P2 into PI5P, but not into PI4P, changes EGFR localization.
  • Fig. S10. PI5P production localizes EGFR to SNX1-positive early endosomes and prevents its routing to lysosomes.
  • Fig. S11. PI5P production does not affect retrograde transport.

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Citation: D. Ramel, F. Lagarrigue, V. Pons, J. Mounier, S. Dupuis-Coronas, G. Chicanne, P. J. Sansonetti, F. Gaits-Iacovoni, H. Tronchère, B. Payrastre, Shigella flexneri Infection Generates the Lipid PI5P to Alter Endocytosis and Prevent Termination of EGFR Signaling. Sci. Signal. 4, ra61 (2011).

© 2011 American Association for the Advancement of Science


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