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Sci. Signal., 27 September 2011
[DOI: 10.1126/scisignal.2002204]

Supplementary Materials for:

Time-Dependent Quantitative Multicomponent Control of the G1-S Network by the Stress-Activated Protein Kinase Hog1 upon Osmostress

Miquel Àngel Adrover, Zhike Zi, Alba Duch, Jörg Schaber, Alberto González-Novo, Javier Jimenez, Mariona Nadal-Ribelles, Josep Clotet, Edda Klipp,* Francesc Posas*

*To whom correspondence should be addressed. E-mail: edda.klipp{at} (E.K.); francesc.posas{at} (F.P.)

This PDF file includes:

  • Model Description
  • Table S1. Overview of experimental design.
  • Table S2. SD of the estimated parameters.
  • Table S3. Initial conditions of the state variables.
  • Table S4. Complete list of model parameter values.
  • References
  • Fig. S1. The timing relationship between Sic1, Cln2, replication, and budding is maintained for stronger stress.
  • Fig. S2. Hog1 activation correlates with a cell cycle arrest in G1 during osmostress in cells synchronized by elutriation.
  • Fig. S3. Patterns of cyclin production and Sic1 degradation are altered during osmostress.
  • Fig. S4. Hog1 activation delays Clb5 production when cells are stressed before Start in a second cell cycle.
  • Fig. S5. Osmostress delays onset of mRNA production of MBF-dependent genes, as well as Clb5 transcripts and protein.
  • Fig. S6. The coarse-grained black box model describing Cln2 and Clb5 production.
  • Fig. S7. Comparison of model simulation result to experimental data used for parameter estimation (in-sample fit).
  • Fig. S8. Validation of model with additional experimental data under other conditions (out-sample fit).
  • Fig. S9. Predictions for the in silico Sic1T173A mutant in response to osmotic stress.
  • Fig. S10. Predictions for the in silico knockout for Cln2 production in response to osmotic stress.
  • Fig. S11. Predictions for the in silico knockout for Clb5 production in response to osmotic stress.
  • Fig. S12. Inactivation of Sln1 leads to accumulation of Sic1- and Hog1-mediated G1 arrest.
  • Fig. S13. Predictions for GAL1::CLN2 (Cln2 overexpression) and GAL1::CLB5 (Clb5 overexpression).
  • Fig. S14. Effect of ectopic expression of CLN2 and CLB5 from the GAL1 promoter on G1 arrest in response to osmotic stress.
  • Fig. S15. Regulation of Clb5 and the onset of replication upon osmostress are independent of the down-regulation of CLN2.

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Other Supplementary Material for this manuscript includes the following:

  • SBML Model S1. Final_Model_for_noStress.xml
  • SBML Model S2. Final_Model_for_Stress_Conditions.xml

[Download SBML Model S1 and S2 (Compressed)]

Technical Details

Format: Microsoft Excel. Files are packaged as a compressed archive, in *.zip format; users should download the compressed file to their machine and decompress the file on their local hard drive, using the instructions below.

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Instructions for downloading and decompressing files:

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Citation: M. À. Adrover, Z. Zi, A. Duch, J. Schaber, A. González-Novo, J. Jimenez, M. Nadal-Ribelles, J. Clotet, E. Klipp, F. Posas, Time-Dependent Quantitative Multicomponent Control of the G1-S Network by the Stress-Activated Protein Kinase Hog1 upon Osmostress. Sci. Signal. 4, ra63 (2011).

© 2011 American Association for the Advancement of Science

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